Postdoc in the Kruse lab, @HMS_BCMP
previously @doudna_lab / Kuriyan lab grad student in @berkeleyMCB
Sep 7, 2021 • 8 tweets • 4 min read
New pre-print! While #CRISPR-Cas9 searches through the genome, how does it actually "read" DNA sequence information, which is normally hidden within the double helix? We determined structures of Cas9 in the act of DNA interrogation to find out! Thread below 👇 (1/8)
A key step in RNA-guided target search is breaking open DNA base pairs next to the PAM. It'd be great to see what that process looks like, but it's difficult to observe because it occurs so quickly (estimated lifetime <30 ms). (2/8)
