Discover and read the best of Twitter Threads about #CRISPR

Most recents (24)

A finales de noviembre de 2018 conocimos el nacimiento de las gemelas chinas con su genoma editado por He Jiankui y luego supimos de una tercera. Tres años más tarde qué sabemos de las 3 niñas que nacieron en China? Interesante investigación de @metricausa para @NatureBiotech ⬇️
Hoy me toca hablar de integridad científica y responsabilidad en Asturias. Precisamente lo que no tuvo He Jiankui. Su gran irresponsabilidad lo llevó a cruzar varias líneas rojas, y el resultado fueron tres niñas editadas de las que apenas sabemos nada. ⬇️
nature.com/articles/s4158…
La ensoñación de He Jiankui pretendía crear niños resistentes al virus VIH, inmunes al SIDA. No lo consiguió. Nacieron tres niñas con su genoma editado. Y con innumerables incertidumbres. Gestionables en modelos animales, pero irresponsables en bebés. ⬇️
nature.com/articles/s4158…
Read 14 tweets
Ever felt your favorite gene has *more than one function* after perturbing it in many cell contexts? Today at #MLCB2021, I'll discuss automatic genotype-phenotype inference from high-dimensional gene perturbation data using sparse approximation: arxiv.org/abs/2111.06247 [a 🧵]
This work was performed with my mentors @marinkazitnik (@HarvardDBMI), James McFarland (@CancerDepMap), and William Hahn (@broadinstitute @DanaFarber)
along with @JKwonBio, Jessica Talamas, @AshirBorah, @aviadt, @paquita, @boehmjesse
Words and genes share a correspondence: their *meanings* are resolved via context. Just as polysemic works like "apple" can refer to fruits or computers depending on the sentence, pleiotropic genes can perform function_1 or function_2 depending on what's going on in the cell.
Read 12 tweets
#Thread Le cauchemar Crispr

L’entreprise lyonnaise GenOway met sur le marché une souris humanisé (génétiquement modifiée avec #Crispr)

Ce « modèle extrêmement innovant » réagit à une infection au Covid de la même manière qu’un humain, jusqu’aux orages cytokiniques.
Les souris transgéniques sont utilisées depuis longtemps pour tester des vaccins et des traitements, sans qu’on ne questionne jamais l’éthique de ces méthodes...

L’usage de Crispr permet de développer des souris GM avec les caractéristiques voulues de manière beaucoup + rapide.
Les souris de Genoway sont dotées d'un système immunitaire humain complet et fonctionnel intégrant des récepteurs ACE2 humains (la porte d’entrée du SARS-CoV-2 dans nos cellules).

Cette souris a été développée en moins de 2 ans alors qu’il en aurait fallu 5 auparavant.
Read 21 tweets
Hoy hemos conocido, por la prensa, un xenotrasplante realizado en EE.UU. en el cual un riñón de un cerdo ha sido trasplantado a la pierna de una mujer declarada en muerte cerebral, y parece haber funcionado durante 54 horas. Voy a intentar explicar este avance médico en un #hilo
Para empezar, los xenotrasplantes son trasplantes de órganos entre especies distintas, por ejemplo entre cerdos y humanos, propuestos desde hace más de 30 años para suplir la falta de órganos de donantes que requieren las personas que necesitan un trasplante y están en espera.
Sin embargo trasladar un órgano entre especies distintas no es nada sencillo, pues el sistema inmunitario del animal receptor normalmente rechazará el órgano de otra especie al detectarlo como un cuerpo extraño/no propio. Y lo rechazará: fulminantemente, a medio y a largo plazo
Read 17 tweets
#Thread sur les chimères animal-homme légalisées par LREM depuis août 2021.

C’est moins mignon que les nounours en chocolat mais c’est un volet majeur et très controversé de la loi bioéthique portée par Agnès Firmin.
La nouvelle loi bioéthique inclue des progrès sociétaux comme l’assistance médicale à la procréation à toutes les femmes et un nouveau mode de filiation pour les enfants de couples de femmes. Mais d’autres dispositions moins médiatisées posent de lourdes questions.
La précédente loi de bioéthique, datant de 2011, interdisait la création d’embryons transgéniques ou chimériques.

Au terme d’un parcours législatif chaotique débuté en 2019, la nouvelle loi bioéthique autorise finalement la création de ce type d’embryons.
Read 27 tweets
#Thread Le réchauffement et le dérèglement climatique ne doivent pas servir d’épouvantails dissimulant les pollutions chimiques et favorisant des projets anti-écologiques.

Le cas du Corail.
Le corail est un animal invertébré qui sécrète un squelette calcaire. Leur accumulation forme ce que nous appelons les récifs.
Selon le dernier rapport de l'Unesco, 20% des récifs coralliens de la planète sont déjà pratiquement détruits, 24% sont exposés à une menace directe liée aux activités humaines et 26% pourraient subir la même menace dans un avenir proche. whc.unesco.org/document/158688
Read 25 tweets
Systems defending microbes (#CRISPR, R-M, etc) from #phages and other mobile genetic elements (MGEs) are increasingly found in MGEs themselves (within cellular genomes). So, who's defending whom from what? An essay ecoevorxiv.org/zuh4c 1/5
Why are there so many defense systems in each genome? It's reasonable to think that they allow multi-layered diverse defenses from phages. But what if they are mostly the result of the multiplicity of MGEs in genomes? 2/5
How do defense systems affect gene flow? Less transfer could be a costly by-product of defense systems. But MGEs have often more to lose than to gain from infection by other MGEs. Lowering gene flow may be a primary reason of selection for defense systems (by MGEs). 3/5
Read 5 tweets
New pre-print! While #CRISPR-Cas9 searches through the genome, how does it actually "read" DNA sequence information, which is normally hidden within the double helix? We determined structures of Cas9 in the act of DNA interrogation to find out! Thread below 👇 (1/8)
A key step in RNA-guided target search is breaking open DNA base pairs next to the PAM. It'd be great to see what that process looks like, but it's difficult to observe because it occurs so quickly (estimated lifetime <30 ms). (2/8)
We used an old chemical trick from the Verdine Lab to staple Cas9 to a piece of DNA that has a PAM but is not complementary to the guide RNA, holding the interrogation complex together long enough for us to do experiments on it. (3/8)
Read 8 tweets
🔻Ne pas confondre

- Thérapie génique (modifie intentionnellement l’ADN du patient)

- Vaccin génique (Ne modifie PAS l’ADN du patient. Utilise un code génétique pour faire produire à l’organisme du patient une protéine [antigène]). journals.openedition.org/rei/3123#tocto…
Les thérapies géniques sont utilisées pour soigner des maladies génétiques et des maladies rares. Elles sont personnalisées.

Le contenu injecté intègre des ciseaux moléculaires, comme #Crispr Cas9, afin de modifier ou remplacer un gène précis du patient infogm.org/7068-deux-nobe…
Le marché mondial des thérapies de modification génétique devrait dépasser les 3,5 milliards $ d'ici 2024.

Le principal lobby des médicaments en Europe est très enthousiaste vis à vis de ce type de produits :
- leem.org/la-therapie-ge…

- leem.org/100-questions/…
Read 5 tweets
"We're talking about [] redesigning organisms... not just edit #genomes but also [], write a new code for life. We'll have write-level permissions. We already started to see some of that this year. #COVID19 #vaccines, they make use of engineered code in the form of #mRNA."
Apr 2021, #WEF: "The new technologies driving the #4IR will be key to the global recovery... but only if they are harnessed properly."

"Synthetic #biology includes #CRISPR, the gene-editing technology currently being used to fight #COVID19."

#mRNA

weforum.org/agenda/2021/04… Image
2019: "More than a technological change, this #biodigital convergence may transform the way we understand ourselves & cause us to redefine what we consider #human or natural."

#4IR #Disruption #Canada



horizons.gc.ca/en/2020/02/11/… ImageImageImage
Read 3 tweets
Scientists are Surprised👁️

When🦟RNA Guides Go Rogue

Surprisingly, the RNA GUIDES ALONE (without Cas13b) also gave substantial suppression. Our study provides support for the potential use of Cas13b in mosquitoes, but also caution in interpreting CRISPR/Cas
Read 4 tweets
"The promise & peril of the Bio Revolution"

"...the world cheered [] that 3 #gene-based COVID19 #vaccines – 1 developed by German #biotech co. BioNTech in collab. w/ Pfizer, another by [] biotech firm Moderna, & a 3rd by [] AstraZeneca – had proved effective in clinical trials."
"But in October, researchers revealed that off-target effects of the #CRISPR-Cas9 #gene-editing tool used to repair a blindness-causing gene in the early stages of human #embryo development often eliminated an entire #chromosome or a large part of it."

#BioengineeringEconomy
"Our deepening knowledge of #biology#genes, #microbiomes, & neural signals – is making it increasingly possible to "engineer life."... #gene drives applied to infectious-disease vectors [] could save many lives, but we may not be able to control them."

#4IR #Biotech
Read 9 tweets
IMPORTANT ‼️

It is in fact possible to get #GrapheneOxide in a clear liquid serum by reducing it's oxygen content. That also increases it's toxicity to humans!

"...treating GO with hydrazine both removes the debris & reduces (both deoxygenations) the graphene sheets".

THREAD: ImageImageImage
Reduction of a clear graphene oxide solution using sodium dithionite. Graphene flakes appear "out of nothing".

See link:
researchgate.net/figure/Reducti…
Read 12 tweets
#COVID19 as catalyst for #4IR #biotech experimentation. The fusion of man & machine sought by the depraved. The foray of #gene editing into public realm. You are the subject.

Jan 24, 2019: "Imperial scientists present #vaccine #revolution to world leaders at #WEF in Davos"
January 2021, TIME, double issue. "The #Vaccine #Revolution" has arrived.

TIME - owned by Marc Benioff, CEO/founder of #Salesforce. World Economic Forum Board of Trustees. Inaugural Chair, Forum Centre for the Fourth Industrial Revolution, San Francisco.

#WEF #4IR #Biotech
#4IR gene-edited, flu-resistant chickens. Working w/ Roslin Inst. Barclay (Imperial) is using #CRISPR technology - to alter the #genome of a species.

April 5, 2020: One of my most thorough, informative & important threads:

Read 4 tweets
A milestone in modern medicine: 1st therapeutic in vivo gene editing in human patients!

From Julian Gillmore, @intelliatweets & colleagues out today @NEJM:

nejm.org/doi/full/10.10…

Here’s an overview of a new potential treatment for transthyretin (ATTR) amyloidosis.

(thread)
2/

Transthyretin (TTR) is expressed in liver, is secreted into blood, and functions as vitamin/hormone transporter.

ATTR amyloidosis is caused by abnormal aggregates of destabilized TTR monomers (normally forms tetramers)—either mutant or wild-type—damaging nerves and/or heart.
3/

Patisiran, developed by @Alnylam, is a lipid nanoparticle (LNP)-delivered siRNA that knocks down TTR mRNA in liver.

In clinical trial, 0.3-mg/kg dose IV every 3 weeks = 81% ⬇️ blood TTR, halting neurologic disease progression with ⬆️ quality of life:

nejm.org/doi/full/10.10…
Read 11 tweets
Just published @NEJM
#CRISPR genome editing for hereditary amyloidosis
Dose-dependent drop of misfolded TTR protein in 6 patients, using mRNA in lipid nanoparticle intravenous infusion.
nejm.org/doi/full/10.10…
This is the 1st IV infusion of #CRISPR genome editing in patients directly into the bloodstream (target is the liver where the misfolded proteins are made); previously for sickle-cell and beta-thalassemia cells were removed from the body and edited, given back
Potential for a 1-time, lifelong treatment extends to many other conditions previously unapproachable. Convergence with mRNA/lipid nanoparticle technology that brought us covid vaccines.
sciencemag.org/news/2021/06/c…
@ScienceMagazine by @jocelynkaiser w/ @doudna_lab @kiranmusunuru
Read 3 tweets
Genome editing with #CRISPR is the most important life science breakthrough of our time. It's a B.C. and A.C. We interviewed @WalterIsaacson about his new book, THE CODE BREAKER, which is a masterpiece medscape.com/viewarticle/95…
w/ transcript /1
We started the conversation reading 2 key passages that convey the significance of #CRISPR /2
And this one /3
Read 11 tweets
Excited to see my first ever first-author paper and the first chapter of my dissertation out now in @ISMEJournal!

We did an exhaustive review/synthesis of what is known about the effects of community context on bacteria-phage ecology and evolution (spoiler: not much)

A🧵(1/n)
If you want a little more detail than a Twitter thread, you can check out a 'behind-the-paper' blog post we wrote here: naturemicrobiologycommunity.nature.com/posts/in-bacte…

(2/n)
There has been a long history of studying bacteria and their viral symbionts, #bacteriophages. In #ecology and #evolution, they've been workhorse systems for studying host-parasite interactions for many years (3/n)
Read 15 tweets
#Crispr Deux groupes de scientifiques travaillant dans de grandes universités américaines tentent de breveter des méthodes « d'édition de génome héréditaire » (modification d’embryons humains pour créer des bébés-OGM qui transmettront leur génome modifié à leurs descendants).
Il s’agit du groupe de Shoukhrat Mitalipov de l'Oregon Health & Science University (OHSU) et du groupe de Dietrich Egli de l'Université Columbia à New York, deux groupes rivaux adeptes de l’eugénisme et de l’amélioration du QI des gens par la modification génétique.
Egli et Mitalipov sont d'éminents scientifiques, largement publiés dans des revues prestigieuses, qui travaillent dans de grandes universités. Ils travaillent donc à breveter des méthodes efficaces pour créer des bébés OGM, comme l’a fait le chinois He Jiankui en 2018.
Read 4 tweets
Two groups of scientists at prominent US universities are trying to patent methods for editing human embryos, with reproductive use clearly intended. Yes, really. (1/7)
geneticsandsociety.org/biopolitical-t…
Reminder: heritable human genome editing (making #CRISPR babies) is prohibited in at least 70 countries! (2/7)
liebertpub.com/doi/10.1089/cr…
Shoukhrat Mitalipov and @OHSUNews have submitted a patent application for “correcting a mutant allele of a gene of interest in… a one-cell embryo and/or a human cell.” (3/7)
appft.uspto.gov/netacgi/nph-Pa…
Read 7 tweets
#Thread Le problème du dérèglement climatique est réel, mais il est étonnant que le problème de la stérilisation en cours de l’espèce humaine provoquée par les ravages de la même industrie pétrochimique fasse si peu parler en comparaison, alors qu’elle pourrait advenir dès 2045
Les recherches menée par Shanna Swan, professeure de médecine à l’hôpital Mount Sinaï de New York, montrent que l’espèce humaine sera infertile en 2045 si l’usage de produits chimiques industriels n’est pas drastiquement diminué d’ici là nytimes.com/2021/03/05/boo…
En France, entre 18 et 24 % des couples ne parviennent pas à avoir un enfant après 12 mois sans contraception. Un chiffre en constante évolution. La principale raison : La présence de substances chimiques toxiques dans l’air et l’alimentation info.arte.tv/fr/lhomme-du-f…
Read 19 tweets
Ultimate victims of media induced fear & politically correct #posturing will be our #children. "hygiene hypothesis: "when exposure to parasites, bacteria, & #viruses is limited early in life, children face a greater chance of [] allergies, asthma, & other #autoimmune diseases..." Image
Dec 10, 2020, Nature: "'#Children are very much adapted to respond — & very well equipped to respond — to new #viruses,' [] even when they are infected w/ #SARSCoV2, children are most likely to experience #mild or #asymptomatic illness." Image
"These children’s #immune systems see the #virus & mounts this really quick & effective immune response that shuts it down, before it has a chance to replicate to the point that it comes up positive on the swab diagnostic test",

Natural #Immunity is not a conspiracy theory.
Read 12 tweets
Daily Bookmarks to GAVNet 05/17/2021 greeneracresvaluenetwork.wordpress.com/2021/05/17/dai…
Battlestar Galactica Lessons from Ransomware to the Pandemic

theinsight.org/p/battlestar-g…

#ransomware #PandemicResponse #LessonsLearned
Female northern elephant seals spend 18 hours a day foraging in deep sea

phys.org/news/2021-05-f…

#FemaleSeals #foraging #BehaviorTracking
Read 10 tweets

Related hashtags

Did Thread Reader help you today?

Support us! We are indie developers!


This site is made by just two indie developers on a laptop doing marketing, support and development! Read more about the story.

Become a Premium Member ($3.00/month or $30.00/year) and get exclusive features!

Become Premium

Too expensive? Make a small donation by buying us coffee ($5) or help with server cost ($10)

Donate via Paypal Become our Patreon

Thank you for your support!