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Kaitlyn Sadtler, PhD Profile picture
Kaitlyn Sadtler, PhD
@KSadtler
Immunologist & Bioengineer, Investigator @NIH @NIBIBgov || #ForbesUnder30 || @TEDFellow || PhD @HopkinsMedicine, Postdoc @MIT (views≠employer)
May 26, 2020 • 10 tweets • 7 min read
Our ELISA validation Breakdown for #SARSCoV2, in short, we validated six (6) ELISAs: spike & RBD IgG, IgM and IgA (check out @cispt2’s summary too!) (1/9) medrxiv.org/content/10.110… Image Great ELISA starts with great protein: @domespo @FredNatLab generated two spike and two RBD constructs from different plasmids, and we found that @NIAIDNews VRC spike ectodomain and @SchmidtLabHMS @ragoninstitute RBD were the ideal antigens. (2/9) Image