Mutation bias (in Arabidopsis thaliana) – is it real? [0/24| Long 🧵addressing a recent critique by Liu & Zhang of our report by @Grey_Monroe et al of a link between epigenomic features & mutation in Arabidopsis that can reduce overall deleterious mutation rate academic.oup.com/mbe/article/39… [1/25]

1/8 16S rDNA amplicons are compositional & do not inform on total abundances. We combine them w/ @Illumina shotgun sequencing to improve detection of microbial interaction networks n plant leaves #microbiome.
@ISMEJournal led by @JulianRegp & @DerekSeveri nature.com/articles/s4139… 2/8 We begin by shotgun sequencing total DNA from leaves of wild plants. Ratios of bacteria/plant reads greatly vary (a), yet in a 30,000 ft view, relative abundance of bacterial families does not seem to be too dissimilar (b)