Cell biologist with an interest in pretty much everything
Feb 12 • 15 tweets • 2 min read
Things I wish more people knew about doing science in the US (thread):
It is *really* hard to get federal funding. NIH grants are an enormous amount of work, requiring extensive scientific, ethical, and budgetary justification for every proposed experiment and dollar spent
Nov 4, 2022 • 7 tweets • 3 min read
Speaking of NAD/NADH (aren't we always?), time to revisit an ultimate #metabolismclassic while I sit at an airport gate: here, a deep dive from Krebs and colleagues dissecting the drivers and implications of the redox ratio in the cytosol and mitochondria pubmed.ncbi.nlm.nih.gov/4291787/
First, the premise: understanding the NAD/NADH ratio gives critical insight into what substrates are consumed and why, but it’s not trivial to directly measure these ratios
Mar 9, 2022 • 9 tweets • 4 min read
Our new work describing a “non-canonical” TCA cycle that is engaged in a cell-state dependent manner is out today in @Naturenature.com/articles/s4158…. Congratulations to lead authors @paigearnold2@__btjackson@GSKGradSchool@MSKCancerCenter. A few highlights:
1. In cultured cells, a portion of citrate is not processed through the “canonical” TCA cycle elucidated by Krebs; rather, citrate is exported to the cytosol where it donates acetyl-CoA, ultimately forming malate for mitochondrial re-import and cycle completion
