The Sato Lab (Kei Sato) Profile picture
東京大学医科学研究所システムウイルス学分野教授 The Genotype to Phenotype Japan (G2P-Japan) Consortium主宰 一般社団法人G2P-Japan代表理事 週プレNEWSでコラム連載中→https://t.co/zLvSNcurTq

Oct 10, 2022, 16 tweets

BREAKING🔔 The 14th paper from G2P-Japan🇯🇵 is out at Cell Host & Microbe @cellhostmicrobe. #Omicron BA.2.75 (aka #Centaurus) seems to be more contagious than BA.5 and more pathogenic than the original BA.2. Please RT. 1/16
cell.com/cell-host-micr…

BA.2.75, emerged in India🇮🇳, is classified as VOC-LUM (variant of concern linage under monitoring) by WHO on July 7. Here we elucidated the transmissibility, sensitivity to antiviral immunity & drugs, and intrinsic pathogenicity of BA.2.75. 2/16
who.int/activities/tra…

BA.2.75 is a descendant of BA.2. However, BA.2.75 is phylogenetically different from BA.5, the currently predominant BA.2 descendant. BA.2.75 spike bears nine substitutions, but only a substitution is shared with BA.5 spike. 3/16

First, our mathematical/statistical analysis revealed that the effective reproduction number ("Re" in the figure) of BA.2.75 is greater than that of BA.5. 4/16

Second, we performed neutralization assay. we showed that:
1⃣ Resistance to 3rd-dose vaccine sera: BA.2.75 = BA.2. 5/16

2⃣ Resistance to 4th-dose vaccine sera: BA.2.75 > BA.2 (1.7-fold). 6/16

3⃣ Resistance to breakthrough BA.1/BA.2 infection sera: BA.2.75 = BA.2. 7/16

4⃣ Resistance to breakthrough BA.5 infection sera: BA.2.75 > BA.5 (1.7-fold). 8/16

5⃣ Resistance to BA.5-infected hamster sera: BA.2.75 >> BA.5 (11-fold!). 9/16

6⃣ Resistance to BA.2.75-infected hamster sera: BA.2.75 << BA.5 (6.4-fold!), suggesting that BA.2.75 is immunogenetically different from BA.5. 10/16

7⃣ We further evaluated the efficacy of 10 therapeutic monoclonal antibodies against BA.2.75. FYI, please see the following thread↓ 11/16

Antiviral activity of three drugs, Remdesivir, Molnupiravir (EIDD-1931) and Paxlovid (Nirmatrelvir) against BA.2.75 was also evaluated. All 3 drugs were effective against BA.2.75. 12/16

We solved the structure of BA.2.75 spike by cryo-electron microscopy and showed how BA.2.75 spike exhibits very high affinity to human ACE2 receptor. 13/16

Like BA.5 spike, BA.2.75 spike is highly fusogenic than BA.2. 14/16

Using clinical isolates of BA.2, BA.2.75, BA.2 & Delta and a hamster model, we demonstrated that the intrinsic pathogenicity of BA.2.75 is comparable to that of BA.5 but is greater than that of the original BA.2. 15/16

▶️ Our multiscale investigations suggest that BA.2.75 acquired virological properties independently of BA.5, and the potential risk of BA.2.75 to global health may be greater than that of BA.5. 16/16

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