Share this page!

Enter URL or ID to Unroll

You can paste full URL like: https://x.com/threadreaderapp/status/1644127596119195649
or just the ID like: 1644127596119195649

How to get URL link on X (Twitter) App

  1. On the Twitter thread, click on or icon on the bottom
  2. Click again on or Share Via icon
  3. Click on Copy Link to Tweet
  4. Paste it above and click "Unroll Thread"!
  5. More info at Twitter Help
Billy Bostickson 🏴👁&👁 🆓 Profile picture
@BillyBostickson
Feb 16, 2021 • 14 tweets • 17 min read • Read on X
Scrolly
122. Revenge of the Sirotkins & hACE2 Mice!
onlinelibrary.wiley.com/doi/10.1002/bi…
This is a "clarification regarding likely leak of a novel viral strain from a Soviet lab" after being harassed by DARPA for publishing:
"Might SARS‐CoV‐2 Have Arisen via Serial Passage?"
onlinelibrary.wiley.com/doi/10.1002/bi… ImageImageImageImage
123. Don't waste your time with the WHO Press Conference in Wuhan, just read this new paper instead:
Open debate on SARS-CoV-2's proximal origin
arxiv.org/abs/2102.03910
@Rossana38510044 @ydeigin @KevinMccairn @Harvard2H @jjcouey @Daoyu15
24 page PDF:
arxiv.org/ftp/arxiv/pape… Image
124. My Two Blind Transgenic Mice Threads
1. From Day 1, SARS-COV-2 was very well adapted to humans .....and transgenic hACE2 Mice

2. High Probability of serial passaging in Transgenic Mice expressing hACE2 in genesis of SARS-COV-2!
125. Domains and Functions of Spike Protein in SARS-CoV-2 in the Context of Vaccine Design
via @TheSeeker268
"the claim that the polybasic furin cleavage site in SARS-CoV-2 has been experimentally inserted is not too far‐fetched."

mdpi.com/1999-4915/13/1… Image
126. SARS-CoV-2 and the Secret of the Furin Site:

preprints.org/manuscript/202…

"The million-dollar question: would a bat coronavirus RatG13, isolated in 2021, have the functional polybasic furin site?" (or like a few years earlier?) via @TheSeeker268 Image
127. Decoding Covid-19 with the SARS-CoV-2 Genome
link.springer.com/article/10.100…
"arguments exist that challenge the zoonotic origin of SARS-CoV-2 and, instead, suggest that the virus may not have a natural origin." ImageImageImageImage
128. Thanks for the reminder from @JohnGal52793009
"Vector sequences in early WIV SRA sequencing data of SARS-CoV-2 inform on a potential large-scale security breach at the beginning of the COVID-19 pandemic"
Daoyu Zhang @Daoyu15 ex- @flavinkins
zenodo.org/record/4486195… ImageImageImageImage
129. @netpoette is back with a comprehensive update
Frustrated by the superficiality & bias of recent press conferences about the possible #origin of #SARS-CoV-2? This article is extremely detailed & comprehensive & updated
changingtimes.media/2020/10/12/sar… ImageImageImageImage
130. Why China and the WHO Will Never Find a Zoonotic Origin For the COVID-19 Pandemic Virus
by Jonathan Latham, PhD and Allison Wilson, PhD
independentsciencenews.org/commentaries/w…
131. Anon, A, Bostickson, B, & Demaneuf, G. (2021) Investigation into Wuhan Institute of Virology Pathogen Databases (Version 2). Zenodo. doi.org/10.5281/zenodo…
zenodo.org/record/4512260…
24 Page on the Missing Databases
My first Paper but not my last, I can assure you! ImageImageImageImage
132. A Bayesian analysis concludes beyond a reasonable doubt that SARS-CoV-2 is not a natural zoonosis but instead is laboratory derived
Quay MD PhD, Steven Carl @quay_dr
zenodo.org/record/4477081…
Massive 193 Pages
Includes a Free Zenodo Warning (never see that before) ImageImageImageImage
133. The German Paper (includes #DRASTIC 50 questions)
Study on the origin of the coronavirus pandemic
researchgate.net/publication/34…
From Roland Wiesendanger's Lab
via @Florian_Hanover and @bej_gff
Study published on the origin of the coronavirus pandemic
uni-hamburg.de/newsroom/press… ImageImageImageImage
134. A thorough analysis and debunking of anti-lab leak arguments by @espo_francesco
Anthropogenic origin of SARS-CoV-2 pandemic
zenodo.org/record/4589116… Image
135. SARS-CoV-2 and the Secret of the Furin Site
preprints.org/manuscript/202…
via @gerdosi
Reviewed here:
news-medical.net/news/20210217/…

• • •

Missing some Tweet in this thread? You can try to force a refresh
　

Keep Current with Billy Bostickson 🏴👁&👁 🆓

Billy Bostickson 🏴👁&👁 🆓 Profile picture

Stay in touch and get notified when new unrolls are available from this author!

Read all threads

This Thread may be Removed Anytime!

PDF

Twitter may remove this content at anytime! Save it as PDF for later use!

Try unrolling a thread yourself!

how to unroll video
  1. Follow @ThreadReaderApp to mention us!

  2. From a Twitter thread mention us with a keyword "unroll"
@threadreaderapp unroll

Practice here first or read more on our help page!

More from @BillyBostickson

Billy Bostickson 🏴👁&👁 🆓 Profile picture
Aug 13
Baric's hACE2 transgenic mice were shared with the WIV for GOF like virus experiments designed to "assess" the potential for coronaviruses to jump from animals to humans.

We asked how many were sent to WIV

researchgate.net/publication/35…

Record via @jhas5 here

arlo.riseforanimals.org/entity/univers… x.com/jhas5/status/1…
2. Baric spills his humanized mice beans to Father Racaniello during a heart rending TWIV confessional in March 2020, admitting he sent his hACE2 mice to WIV in 2018 (as part of the infamous NIAID grant mentioned above):



Timestamp; 01:00:30 - 01:03:15microbe.tv/twiv/twiv-591/
3. Daszak

"Our R01 work shows that bats in South China harbor many SARSr-CoVs (50+), some of which are able to use human ACE2 to enter human cells, infecting humanized mouse models to cause SARS-like illness, and evade available therapies or vaccines"

ecohealthalliance.org/wp-content/upl…
Read 9 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Aug 11
1. New England Biolabs & Novagen

The amplified Rp3-S1 fragment was digested with BamHI & XhoI & ligated into the expression vector pMAL-C2X (New England Biolabs).

The 5 truncated fragments were digested with BamHI & XhoI and ligated into the expression vector pET32a (Novagen).
2. Promega & Takara

Protein expression & purification

After purification, recombinant plasmid was transformed into BL21 competent cells (purchased from Promega)

Recovered PCR product & expression vector pET32a were digested with restriction endonucleases BamHI & XhoI (Takara).
3. Takara (2)

After DNA recovery, the PCR product was ligated to the expression vector pET32a using T4 DNA ligase (Takara)
Read 32 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 26
@breakfast_dogs @gadboit @NLink247 1. Insect cell baculovirus cell cultures become contaminated with insect genomic material.
2. Replication process can lead to presence of viral DNA & cellular components in culture medium.
3. This LEADS to contamination of viral product with baculovirus & insect cell-derived DNA.
@breakfast_dogs @gadboit @NLink247 Contamination

4. Expression of SC-2 Spike RBD Using the Insect Cell-Baculovirus System

pmc.ncbi.nlm.nih.gov/articles/PMC97…

5. Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture

pmc.ncbi.nlm.nih.gov/articles/PMC71…

6. insect cell lines
sciencedirect.com/science/articl…
@breakfast_dogs @gadboit @NLink247 Did WIV use insect cell systems with SARS-C0V-2?

7. Insect cell baculovirus expression systems

x.com/BillyBostickso…

8. DENG Fei, Chief Investigator in Research Group on Viruses Resources at WIV engineered baculovirus-insect cell expression systems.

buzzchronicles.com/b/all/17652/
Read 13 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 22
1. Kadlec awaiting confirmation for his new role

2. Kadlec's report references pangolins 76 times

bush.tamu.edu/wp-content/upl…

3. Time to read my pangolin coronavirus report?

researchgate.net/publication/39…

4. DRASTIC research cited
@gdemaneuf @ydeigin @BillyBostickson @C_Small_ x.com/BoDeepest/stat…
1. the article linked is quite good, more comments and analysis will be posted here soon

city-journal.org/article/robert…Image
2. Possible lab manipulations

"2) insertion of a pangolin virus recombinant spike or the spike’s RBD protein that includes a sequence for an FCS and an integrin-binding protein" Image
Read 76 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 9
Pressure is needed on people like @realannapaulina @RepLuna & @GOPoversight team as their mandate may dissolve in August.

They did Kennedy, but they are supposed to do UFO files & CoVid-19 origins before they dissolve.

We also need to know about the subpoenas sent by @RandPaul
What was promised and demanded (for the record)

oversight.house.gov/release/rep-lu…

oversight.house.gov/release/chairm…
If you are a concerned US citizen please directly contact the people listed here:

oversight.house.gov/subcommittee/t…
Read 8 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
Jul 1
🧵Rev. Billy's Lab Leak Cover-Up Sermon

1. Brethren, hear me now, for we stand at the precipice of a great & grievous iniquity!

The world has been ensnared by a monstrous conspiracy, a vile machination spun by a clique of scientists, experts, media scribes & state overlords!
🧵2. Let us never forget!

Those who, in their arrogance, have sought to veil the truth of the COVID pestilence!

And I say to you today, that this is no mere error, but a deliberate, diabolical plot to obscure the origins of a plague that has scourged the nations!
🧵3. The Vocabulary of Deception

Let us expose this falsehood, this house of deceit

& the duties of the vigilant in these perilous times!

Oh, what a wretched lexicon these deceivers employ! Their words are cloaked in the garb of science, yet they reek of falsehood & treachery!
Read 23 tweets

Did Thread Reader help you today?

Support us! We are indie developers!

This site is made by just two indie developers on a laptop doing marketing, support and development! Read more about the story.

Become a Premium Member ($3/month or $30/year) and get exclusive features!

Become Premium

Don't want to be a Premium member but still want to support us?

Make a small donation by buying us coffee ($5) or help with server cost ($10)

Donate via Paypal

Or Donate anonymously using crypto!

Ethereum

0xfe58350B80634f60Fa6Dc149a72b4DFbc17D341E copy

Bitcoin

3ATGMxNzCUFzxpMCHL5sWSt4DVtS8UqXpi copy

Thank you for your support!

Follow Us!

Send Email!

:(