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Coroldo1 Profile picture
@coroldo1
Mar 7, 2021 • 16 tweets • 17 min read • Read on X
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#SARSCOV2 #origin This thread is about analysis of origin search of SARS-COV-2 in various outbreaks in China. The 'origin search' is more for epi purpose, not quite similar as the 'origin research' in viral evolution. Of course, first cases and Huanan market include here.
1/n
#SARSCOV2 #origin First, let's have some taste of the mutation analysis of full genome sequencing with some covid-19 patients in Wuhan, by #IVDC
2/n ImageImageImageImage
#SARSCOV2 #origin Then, the set up of task force to assess the food safety risk of potential transmission via cold chain.
3/n ImageImageImageImage
#SARSCOV2 #origin
Initial results of the first batch of samples from Wuhan on 2nd January 2020. After Visionmedicals & BGI got their preliminary results ID as SARSr-CoV. 4/n ImageImageImageImage
#SARSCOV2 #origin
Initial results of the 2nd batch of samples from Wuhan on 2nd January 2020. Received GMT+8 23:00, MiSeq. 5/n ImageImage
#SARSCOV2 #origin seven specimens from severe pneumonia patients in Beijing hospital used as control. 6/n Image
#SARSCOV2 #origin
Just break in for Huanan market a bit. We all knew an environmental origin searching working plan against Huanan seafood market was drafted on 1st Jan, 2020. And leading person of this environmental sampling, Jun Liu, also is the deputy of George Gaofu's lab. Image
#SARSCOV2 #origin
a bit background story by China CDC own declaration, pay attention
a) full sequence obtained on 3rd Jan.
b) testing protocol established on 4th Jan.
c) shared genome and image via GISAID on 12th Jan.
d) a bit description of Jun Liu. 6/n

chinacdc.cn/yw_9324/202003…
#SARSCOV2 #origin
These results of environmental sampling and analysis can also found in publications.7/n ImageImage
#SARSCOV2 #origin
Analysis of initial samples .8/n ImageImageImageImage
#SARSCOV2 #origin
Analysis of initial samples .9/n ImageImageImageImage
#SARSCOV2 #origin
Analysis of initial samples. 10/n ImageImageImageImage
#SARSCOV2 #origin
Analysis of initial samples. 11/n Image
#SARSCOV2 #origin
Various outbreaks in China & COLD CHAIN. 12/n ImageImageImageImage
#SARSCOV2 #origin
Various outbreaks in China & COLD CHAIN. 13/n ImageImageImageImage
#SARSCOV2 #origin
Various outbreaks in China & COLD CHAIN. 14/n ImageImageImage

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More from @coroldo1

Coroldo1 Profile picture
Mar 31, 2023
@martinenserink
The snapshots contain rich information.
1. 'it is a novel coronavirus' = distinctive from SARS1, must have been successfully isolated from patient samples, must have been verified (revisit the *chlamydia* in SARS1)
@martinenserink 2. W/o knowing the exact contents, based on the low resolution image and file name of the pptx, predated Virological. That alone, already can be treated as a high confidence evidence that China, through GISAID, was providing a unique additional info regarding the novel cov to WHO
@martinenserink 3. By a glance of the low resolution image of pptx, a possible standard NJ tree of ORF1b. Normally, considering the file size, one page ppt would incl the details of tree creation, against CoV family or particular strains, and *source(s)* of the colour highlighten genomes.
Read 48 tweets
Coroldo1 Profile picture
Mar 29, 2023
chinaxiv.org/abs/202303.103…
Would love to have a comparison of June 2022 revision and today's new release on ChinaXiv.
If available. So, pls, that 'reviewer who claims to have reviewed the manuscript for Nature'.
I am not caring too much for the conclusion part, as this new version seems targeting for expedited peer-review process. We all see 'toned down' with previous preprints vs publications.
What's important was the comparison of results in those versions.

If the June 2022 vs ChinaXiv, not much difference, and new version get published. Then, I think not CCDC but someone else get explanation to do.
Read 7 tweets
Coroldo1 Profile picture
Mar 28, 2023
Is it an established fact that no one on twitter or press seem bother emphasising? that:
1. In SAGO meeting, the original data generators said they already *KNEW* the raccoon dogs etc al sequence in the data they produced (before lifted embargo) , and was in the middle of
submitting a revision.
2. The coauthor(obviously not the communication authors with email listed and mobile number too well known to *everyone*) of both preprint and initial illumina data& BGI data, expressed during the initial contact, that(pls pay attention)
*we were told to release the data....feel free to analyse....happy to answer any questions... *.
Read 5 tweets
Coroldo1 Profile picture
Mar 27, 2023
A brief 'announcement'.😹 Busy with something more important, just break in briefly.
1. Pls, don't want to reply to the DMs flooded in, until we see the origonal data generators' own publication.
2. Accuracy...... If we want to avoid accusations of *power of media*, perhaps anyone point to me the reference of this specific usage implied transferring live animals, 'In one sample from a cart used to move cages', as if the reporter was there......
nature.com/articles/d4158…
3. I *strongly believed* that the original environmental sampling documents and records were written in *Chinese*, no? Why not?
So, if u googled 脱毛机, isn't human epilators all over ur screen? I knew it's hard for some male reviewer #3 to comprehend🤭.

Read 7 tweets
Coroldo1 Profile picture
Mar 4, 2023
*Working diagnostics* for Covid-19 were *only available* *after* *mid-Jan*. But the *Chinese gov* had issued order*s* to *destroy* *all patient* samples *on Jan 3, 2020*.

I will do a mini thread to pointed out how factually wrong and misleading the layman readers, regarding ☝️
When I have time this weekend, I will present the original materials, references, word by word.
Btw, can anyone reply with ur copy of that 2020-03 'gag order' full document, just as reference to present as comparison, thank you.

To intrigue some interest, let me present you the newly issued 'gag order' [2023-13]by the same office of NHC which issued the 2020-03 'gag order'. Just for fun.
Read 32 tweets
Coroldo1 Profile picture
Mar 3, 2023
A mini thread about this breaking news, in short, it's not a breaking news......
I remembered EISS was conducting some epidemiological intelligence workshops, correct me if I am wrong, so, source finding->

So, we all know Chinese national center of influenza posted weekly flue press, like any other countries such as EU, US, even HONG KONG S.A.R,
We all familiar with this, right?
So, on this weekly(6th-12th Feb) report of week6year2023, published on 17th Feb.
It stated a human H5N1 case reported in Jiangsu province.

ivdc.chinacdc.cn/cnic/zyzx/lgzb…
Read 11 tweets

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