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V. Courtier Orgogozo

2 Aug, 5 tweets, 4 min read

@TheSeeker268

@TheSeeker268 ZL Shi indicated in July 2020 in Science that the 5' end of RaTG13 was missing from their sequence: sciencemag.org/sites/default/…

@TheSeeker268

@TheSeeker268 Note: how did they know that the missing 5' piece was 15 nucleotide long in July 2020?

@TheSeeker268

@TheSeeker268 and then, on 13 October 2020 the missing 15bp appeared in NCBI, with no explanation & no supporting raw data
ncbi.nlm.nih.gov/nuccore/MN9965…
as noticed by @Ayjchan

https://twitter.com/Ayjchan/status/1317957535274569728?s=20

@TheSeeker268

@TheSeeker268 @Ayjchan These 15bp at the RaTG13 5'end are identical to the ones found in SARS-CoV-2. It is unclear how this sequence was obtained.

@TheSeeker268

@TheSeeker268 @Ayjchan I emailed Zheng-Li Shi several questions about RaTG13 but got no answer.
virginiecourtier.wordpress.com/covid-origin/

I am surprised that most virologists do not seem to care about the issues surrounding RaTG13.

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V. Courtier Orgogozo

@Biol4Ever

10 May

@K_G_Andersen

@K_G_Andersen Thank you so much for taking the time to lay down your arguments about this furin cleavage site. This is very useful and highly needed.
Yes, there is one aspect that you are missing, which has been bothering me:
1/

@K_G_Andersen

@K_G_Andersen This FCS was noted as "cleavage site" in a January 2020 publication by Zheng-Li Shi and colleagues (before the Nature paper):
"we predicted that the cleavage site for generating S1 and S2 subunits is located at R694/S695"
ncbi.nlm.nih.gov/pmc/articles/P…
2/

@K_G_Andersen

@K_G_Andersen Note that the correct position of the R/S furin cleavage site is 685/686 and not 694/695. There is no other RS amino acid sequence in the neighboring region so this "R694/S695" points to the furin cleavage site.
3/

Read 8 tweets

V. Courtier Orgogozo

@Biol4Ever

1 Feb

@cathyqqtao

One of the best papers I read in the last few years:
Sudhir Kumar @cathyqqtao @stvnwvr @sergeilkp & al.
reconstructed virus evolution using cancer mutation lineage tracing approach @TempleUniv
@biorxivpreprint

biorxiv.org/content/10.110…

The authors examined #SARSCoV2 evolution and found that all sequences originate from
proCoV2, the mother genome of all #SARSCoV2 sequences.
The reference SARSCoV2 sequence from 24 December (Wuhan‐1;EPI_ISL 402123) has 3 mutations compared to proCoV2.
2/

The progression from proCoV2 to Wuhan-1 via 3 successive mutations is supported by >850 intermediate genomes.
The mother genome was detected in China and USA until March 2020.
3/

Read 5 tweets

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V. Courtier Orgogozo

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