HiC from @PhaseGenomics on Psilocybe cubensis.
204 Million 150bp reads (30 billion bases) are generated to produce a Proximity ligation map of the cubensis genome. The Blue boxes are the 13 Chromosomes.Most of which have telomeres now.
13 Chromosomes from 4.675Mb to 2.3Mb in size.
The dense diagonal lines represent the likelihood of those regions of the genome being in close proximity to other regions.
Since chromosomes tend to be wound by chromatin, cross linking of the chromatin, followed by digestion
And ligation provides you with chimeric DNA that represents the 3D structure of the DNA as it’s wrapped around chromatin.
Pieces of DNA, 1 million bases apart but on the same chromosome, are more likely to be captured in this sequencing process.
Better resolution images.
More likely to be capture in the sequencing process if they are on the same chromosome than if they are on different chromosome.
The nature of proximity ligation provides you a map of which contigs are on the same chromosomes.
The genome was in 32 contigs after @infoecho assembled it.
The HiC data just organized those 32 pieces into 13 chromosomes and helped up places some of the shrapnel Telomeric contigs onto their respective chromosomes.
We keep hearing from @drsanjaygupta that the vaxxed are less likely to get C19.
Not true for many age groups.
This was evident in the Pfizer trial data. There is negative VE 2 weeks after the shot.
I hope @drsanjaygupta reviews the references on myocarditis.
It’s 1:5000 from the Vax.
1:1M from the virus.
Asymptomatic spread is NOT 40% of cases. Iceland showed kids don’t transmit it.
You don’t have immunity from the Vax.
Antibodies levels are poor proxy for outcomes.
Myocarditis in kids.
You are pimping dangerous medicine that is being mandated. You are on the wrong side of history and show up to Joe Rogan better prepared with citations …not CNN talking points.