1/n Illumina But With Nanopore! New work from my lab led by all four of my graduate students @waddlingmind, @alexzee111, @MattAtoms, and @dorieD18. Ever had an Illumina library and wanted to sequence it right now but also kinda didn't want a ton of errors? biorxiv.org/content/10.110…
2/n You can now do that on an ONT MinION. Just convert it with R2C2 first. You'll get accuracy and reads/$ comparable to Illumina iSeq/MiniSeq/MiSeq. We benchmarked this approach on RNA-seq, ChIP-seq, regular and targeted-enriched Tn5 libraries.
3/n Our takeaway from this study is that a ONT MinION can definitely replace benchtop Illumina sequencers for most library QC and smaller scale experiments. A cool feature is that thanks to @waddlingmind's PLNK tool, libraries can be QC'ed in real-time often in under an hour.
4/n Looking at such a diverse array of libraries wouldn't have been possible without our collaborators @RussCorbett (core-pivotal author), @shelbilrussell, and @Schmitz_Lab. Now I can finally claim to be an expert on Drosophila, Wolbachia, and soybean biology. #goals
5/n I didn't think we would still be working with R2C2 for this long when @velociroger developed it in 2017 but it has been surprisingly long-lived and versatile. Who knew rolling circle amplification would be so powerful.
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