1. Thread on #DARPA History - Synthetic Polio Virus

Created under the DARPA Biological Warfare Defense Program

web.archive.org/web/2014011302…
2. Multi-agent/agent specific thrust

"the approach. will be targeted to a specific pathogen, but It Is applicable to several different pathogens. For example, an approach may be used to develop therapeutics against 20 different pathogens"
3. Professor Ron Taylor

This project started in May 1996 at the University of Virginia. Taylor and his colleagues developed a heteropolymer that functions on the one end to connect to the CR 1 site of the red blood cells, while the other end is tailored to the specific pathogen.
4. Mesenchymal Stem Cells

DARPA also funded research into mesenchymal stem cells (MSCs) modified to have surface receptors designed for specific pathogens. When pathogen is detected, part of cell's DNA Is turned on to produce a gene protein product targeted to kill the pathogen
5. Strategy

To address specific Biological warfare agents, we will fund work at facilities such as USAMRIID, where actual bioagents are. kept. to determine If the therapeutics are effective In animal models.
6. Pharmaceutical Industry

DARPA will provide the thrust & the biotech industry & pharmaceutical industries will take over these products (clinical testing & development) to commercialize them for their own purposes.

DoD will then procure them via "normal" commercial routes
7. Unconventional Pathogen Countermeasures

"The most sinister offensive biological warfare scenario employs surprise, Immediate proximity &
rapidly lethal, persistent agents in overwhelming quantities"

BAA 97-23 Pathogen Countermeasures

Program Manager: CPR Shaun Jones, MD,USN
8. Advanced. Diagnostics

Program Manager: Dr. Stephen Morse

BAA 97-24 Advanced Diagnostics for Pathogens
9. Red Blood Cell Pathogen Decoy & Destruction
University of Virginia
Boston University - Mark Bitensky birensky@enra.bu.etlu
10. Sequential Auto Vaccination by Stem Cells

Daniel R. Marshak
dmarshak@osiristx.com
OSIRIS Therapeutics, Inc. .
Baltimore, MD
11. Too Radical for NIH? Try DARPA

@Peterdaszak #DEFUSE

You have a radically new idea for fighting pathogens that colleagues are dubious about
Where would you go for funding?
To the NIH?
NIH would ask a committee of peers to evaluate it who can be brutal about radical concepts
12. Scripps - DARPA - Lerner

Richard Lerner, president of The Scripps Research Institute, another DARPA adviser, notes that the agency needs to keep in mind that ''you never know what you want to discover until you discover it."

en.wikipedia.org/wiki/Richard_L…
13. Dr. Chris Dickey

publichealth.nyu.edu/faculty/chris-… ?

DARPA was unable to locate any documents concerning "human cellular platforms used for biological warfare therapy," as you requested.

DARPA advises that this division does not exist, nor does DARPA have such a research program
14.Synthetic Polio

DARPA failed to inform anyone that one of its “unconventional” projects was $US300,000 ($393,939) to fund a trio of scientists who thought it would be a neat idea to synthesise polio.

gizmodo.com.au/2016/03/the-10…
15. DIY Polio @antonioregalado

They constructed the virus using its genome sequence, which was available on the Internet, and obtained the genetic material from companies that sell made-to-order DNA.

ph.ucla.edu/epi/bioter/cre…
16. Frightening the Public!

"I think this is irresponsible," says Craig Venter

He says the work carries an alarmist message that could frighten the public & prompt legislators to put more controls on basic research

"It has the chance to hurt the entire scientific community"
17. #DARPA's Secret

Dr. Wimmer's work was funded with $160K from DARPA, while he served as an adviser

DARPA didn't disclose this project among its grants under the Unconventional Pathogen Countermeasures program

Dr. Wimmer isn't sure why the agency kept the project secret...
18. #DARPA's unpublished projects

"A Darpa spokesman said not all the agency's work is posted on its Web site"

ROLF

darpa.mil
19. Dr. Cello's DNA Lego Building Blocks

But building up the long chain of DNA from smaller pieces proved frustratingly difficult. Eventually, Dr. Cello simply ordered most of the completed sequence from a scientific supply house, Integrated DNA Technologies of Coralville, Iowa
20. Interlude with Dr. Cello

21, More Dr. Cello!

22. Back to Synthetic Polio @DARPA

DARPA provided $300K over the last 3 years for the work

''Understanding the process of viral DNA production is key to identifying new ways to kill viruses & understand how viruses could change & escape from vaccines"

archive.ph/0G7rP
23. Mice Brains

The new virus, when injected into the brains of mice, gave them a paralytic disease equivalent to poliomyelitis.

Dr. Wimmer deliberately introduced mutations into it to distinguish it from the natural virus.

archive.ph/0G7rP By @AndrewPollackFL
24. A virus with a future?

2000-2025

Dr. Wimmer said polio could spread panic & that vaccines would have to be stockpiled if not routinely administered

"in 25 years, if a good percentage of the world is not immunized against polio virus, it would be a terrible terrorist agent"
25. Papers (Don't try at home)
1.
researchgate.net/publication/11…

2. Cell-free de novo synthesis of poliovirus
pubmed.ncbi.nlm.nih.gov/1661029/

3. Recombination of poliovirus in cell-free system
pnas.org/content/94/25/…

4. Cell-free synthesis poliovirus (145 subunits)
citeseerx.ist.psu.edu/viewdoc/downlo…
26. Reactions (1)

Reflections on the Synthetic production of Polio Virus

journals.sagepub.com/doi/pdf/10.296…

Bulletin of the Atomic Scientists @BulletinAtomic
27. Reactions (2)

Congressional Research Service Report RS21369

Synthetic Poliovirus: Bioterrorism and Science Policy
Implications

Frank Gottron (December 13, 2002)

file.wikileaks.org/file/crs/RS213…
28. Synthetic Poliovirus & Other Designer Viruses

What Have We Learned from Them?

Eckard Wimmer: ewimmer@ms.cc.sunysb.edu
Aniko V. Paul: apaul@notes.cc.sunysb.edu

Stony Brook University

annualreviews.org/doi/abs/10.114…

Non paywall PDF thanks to Sci Hub

sci-hub.ee/10.1146/annure…
29. Synthetic Biology Could Bring a Pox on Us All

wired.com/story/syntheti…
30. From Synthetic Polio to Horsepox

Biosecurity Implications for the Synthesis of Horsepox, an Orthopoxvirus

wmdcenter.ndu.edu/Portals/97/Doc…

Step-by-step horsepox study stokes dual-use controversy

cidrap.umn.edu/news-perspecti…
31. Synthetic horsepox viruses

the continuing debate about dual use research

journals.plos.org/plospathogens/…

Horsepox and the need for a new norm, more transparency, and stronger oversight for experiments that pose pandemic risks

journals.plos.org/plospathogens/…
32. Horsepox and Polio

unroll DARPA's secret Synthetic Polio Program

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More from @BillyBostickson

Sep 11
1. "We’ll be seen as spies, not scientists.”

x.com/emilyakopp/sta…

Daszak "seems" confused when questioned BY @RepJamesComer whether he ever worked with, or for, spooks.

Unsurprisingly in light of what is shown on page 79 of the DRASTIC EHA files

researchgate.net/publication/39… x.com/Dylan_Housman/…Image
2. He may claim it was post pandemic contact

But thanks to @AGHuff we know it went back quite a while, at least to 2015.

@AGHuff 3. More questions from @NameIsSpartacus

We need to find out why Peter Daszak & EcoHealth had In-Q-Tel backing and why Nathan Wolfe (an obvious intelligence asset with links to Epstein/Maxwell, Boris Nikolic, and Bill Gates) was on their editorial board.

Read 14 tweets
Sep 10
Good to see Generation Z in Asia growing a set of balls and fighting back against shit governments.



#NepalGenZProtest #Myanmar #SriLanka

Hopefully #China will be next!
Just 3 years ago #SrilankaProtest

One year ago #BangladeshProtest

Read 10 tweets
Sep 6
🧵My comments and some conclusions regarding the EHA documents we found:

1. Peter Daszak actively schemed to foil congressional and other investigations into EcoHealth Alliance (EHA) and its ties to the Wuhan Institute of Virology, as revealed in the DRASTIC document stash.
2⃣ He orchestrated a “Coalition of Support” with former USAID officials like Dennis Carroll and NGOs like the Smithsonian, which sent letters (e.g., April 24, 2020, to NIH) decrying grant cuts as political attacks to deflect scrutiny (Part 6, p.35-36).
3⃣ Daszak hired the Kyle House Group (KHG) to lobby against NDAA defunding amendments, with KHG’s Porter joining meetings like one on August 4, 2023, with Rep. Adam Smith’s staff to kill Sen. Joni Ernst’s provisions (Part 7, p.52).
Read 16 tweets
Aug 22
1. Nord Stream Update

3 years for truth to emerge

@MerlynThomas on 27/09/22 for @BBCWorld

Ukraine accuses Russia of pipeline terror attack

bbc.com/news/world-eur…

@JamesCrisp6 on 28/09/22 for @Telegraph

Why Putin would want to blow up Nord Stream 2

archive.ph/JCtYB x.com/Panchenko_X/st…
2. Hilariously

Merlyn Thomas has been working for the "BBC disinformation unit" since September 2021.

He certainly earned his UK STATE propaganda pay check with Nord Stream!

3. Invitation to venture down a Baltic rabbit hole!

You are hereby invited to read my 682 tweet thread investigating all angles of the Nord Stream sabotage, which ends here:



Thanks @Erkperk @VasilijProzorov @JeffreyBrodsky5 @MichaKobs @bopanc @liscer72
Read 4 tweets
Aug 13
Baric's hACE2 transgenic mice were shared with the WIV for GOF like virus experiments designed to "assess" the potential for coronaviruses to jump from animals to humans.

We asked how many were sent to WIV

researchgate.net/publication/35…

Record via @jhas5 here

arlo.riseforanimals.org/entity/univers… x.com/jhas5/status/1…
2. Baric spills his humanized mice beans to Father Racaniello during a heart rending TWIV confessional in March 2020, admitting he sent his hACE2 mice to WIV in 2018 (as part of the infamous NIAID grant mentioned above):



Timestamp; 01:00:30 - 01:03:15microbe.tv/twiv/twiv-591/
3. Daszak

"Our R01 work shows that bats in South China harbor many SARSr-CoVs (50+), some of which are able to use human ACE2 to enter human cells, infecting humanized mouse models to cause SARS-like illness, and evade available therapies or vaccines"

ecohealthalliance.org/wp-content/upl…
Read 9 tweets
Aug 11
1. New England Biolabs & Novagen

The amplified Rp3-S1 fragment was digested with BamHI & XhoI & ligated into the expression vector pMAL-C2X (New England Biolabs).

The 5 truncated fragments were digested with BamHI & XhoI and ligated into the expression vector pET32a (Novagen).
2. Promega & Takara

Protein expression & purification

After purification, recombinant plasmid was transformed into BL21 competent cells (purchased from Promega)

Recovered PCR product & expression vector pET32a were digested with restriction endonucleases BamHI & XhoI (Takara).
3. Takara (2)

After DNA recovery, the PCR product was ligated to the expression vector pET32a using T4 DNA ligase (Takara)
Read 32 tweets

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