1. Thread on #DARPA History - Synthetic Polio Virus

Created under the DARPA Biological Warfare Defense Program

web.archive.org/web/2014011302…
2. Multi-agent/agent specific thrust

"the approach. will be targeted to a specific pathogen, but It Is applicable to several different pathogens. For example, an approach may be used to develop therapeutics against 20 different pathogens"
3. Professor Ron Taylor

This project started in May 1996 at the University of Virginia. Taylor and his colleagues developed a heteropolymer that functions on the one end to connect to the CR 1 site of the red blood cells, while the other end is tailored to the specific pathogen.
4. Mesenchymal Stem Cells

DARPA also funded research into mesenchymal stem cells (MSCs) modified to have surface receptors designed for specific pathogens. When pathogen is detected, part of cell's DNA Is turned on to produce a gene protein product targeted to kill the pathogen
5. Strategy

To address specific Biological warfare agents, we will fund work at facilities such as USAMRIID, where actual bioagents are. kept. to determine If the therapeutics are effective In animal models.
6. Pharmaceutical Industry

DARPA will provide the thrust & the biotech industry & pharmaceutical industries will take over these products (clinical testing & development) to commercialize them for their own purposes.

DoD will then procure them via "normal" commercial routes
7. Unconventional Pathogen Countermeasures

"The most sinister offensive biological warfare scenario employs surprise, Immediate proximity &
rapidly lethal, persistent agents in overwhelming quantities"

BAA 97-23 Pathogen Countermeasures

Program Manager: CPR Shaun Jones, MD,USN
8. Advanced. Diagnostics

Program Manager: Dr. Stephen Morse

BAA 97-24 Advanced Diagnostics for Pathogens
9. Red Blood Cell Pathogen Decoy & Destruction
University of Virginia
Boston University - Mark Bitensky birensky@enra.bu.etlu
10. Sequential Auto Vaccination by Stem Cells

Daniel R. Marshak
dmarshak@osiristx.com
OSIRIS Therapeutics, Inc. .
Baltimore, MD
11. Too Radical for NIH? Try DARPA

@Peterdaszak #DEFUSE

You have a radically new idea for fighting pathogens that colleagues are dubious about
Where would you go for funding?
To the NIH?
NIH would ask a committee of peers to evaluate it who can be brutal about radical concepts
12. Scripps - DARPA - Lerner

Richard Lerner, president of The Scripps Research Institute, another DARPA adviser, notes that the agency needs to keep in mind that ''you never know what you want to discover until you discover it."

en.wikipedia.org/wiki/Richard_L…
13. Dr. Chris Dickey

publichealth.nyu.edu/faculty/chris-… ?

DARPA was unable to locate any documents concerning "human cellular platforms used for biological warfare therapy," as you requested.

DARPA advises that this division does not exist, nor does DARPA have such a research program
14.Synthetic Polio

DARPA failed to inform anyone that one of its “unconventional” projects was $US300,000 ($393,939) to fund a trio of scientists who thought it would be a neat idea to synthesise polio.

gizmodo.com.au/2016/03/the-10…
15. DIY Polio @antonioregalado

They constructed the virus using its genome sequence, which was available on the Internet, and obtained the genetic material from companies that sell made-to-order DNA.

ph.ucla.edu/epi/bioter/cre…
16. Frightening the Public!

"I think this is irresponsible," says Craig Venter

He says the work carries an alarmist message that could frighten the public & prompt legislators to put more controls on basic research

"It has the chance to hurt the entire scientific community"
17. #DARPA's Secret

Dr. Wimmer's work was funded with $160K from DARPA, while he served as an adviser

DARPA didn't disclose this project among its grants under the Unconventional Pathogen Countermeasures program

Dr. Wimmer isn't sure why the agency kept the project secret...
18. #DARPA's unpublished projects

"A Darpa spokesman said not all the agency's work is posted on its Web site"

ROLF

darpa.mil
19. Dr. Cello's DNA Lego Building Blocks

But building up the long chain of DNA from smaller pieces proved frustratingly difficult. Eventually, Dr. Cello simply ordered most of the completed sequence from a scientific supply house, Integrated DNA Technologies of Coralville, Iowa
20. Interlude with Dr. Cello

21, More Dr. Cello!

22. Back to Synthetic Polio @DARPA

DARPA provided $300K over the last 3 years for the work

''Understanding the process of viral DNA production is key to identifying new ways to kill viruses & understand how viruses could change & escape from vaccines"

archive.ph/0G7rP
23. Mice Brains

The new virus, when injected into the brains of mice, gave them a paralytic disease equivalent to poliomyelitis.

Dr. Wimmer deliberately introduced mutations into it to distinguish it from the natural virus.

archive.ph/0G7rP By @AndrewPollackFL
24. A virus with a future?

2000-2025

Dr. Wimmer said polio could spread panic & that vaccines would have to be stockpiled if not routinely administered

"in 25 years, if a good percentage of the world is not immunized against polio virus, it would be a terrible terrorist agent"
25. Papers (Don't try at home)
1.
researchgate.net/publication/11…

2. Cell-free de novo synthesis of poliovirus
pubmed.ncbi.nlm.nih.gov/1661029/

3. Recombination of poliovirus in cell-free system
pnas.org/content/94/25/…

4. Cell-free synthesis poliovirus (145 subunits)
citeseerx.ist.psu.edu/viewdoc/downlo…
26. Reactions (1)

Reflections on the Synthetic production of Polio Virus

journals.sagepub.com/doi/pdf/10.296…

Bulletin of the Atomic Scientists @BulletinAtomic
27. Reactions (2)

Congressional Research Service Report RS21369

Synthetic Poliovirus: Bioterrorism and Science Policy
Implications

Frank Gottron (December 13, 2002)

file.wikileaks.org/file/crs/RS213…
28. Synthetic Poliovirus & Other Designer Viruses

What Have We Learned from Them?

Eckard Wimmer: ewimmer@ms.cc.sunysb.edu
Aniko V. Paul: apaul@notes.cc.sunysb.edu

Stony Brook University

annualreviews.org/doi/abs/10.114…

Non paywall PDF thanks to Sci Hub

sci-hub.ee/10.1146/annure…
29. Synthetic Biology Could Bring a Pox on Us All

wired.com/story/syntheti…
30. From Synthetic Polio to Horsepox

Biosecurity Implications for the Synthesis of Horsepox, an Orthopoxvirus

wmdcenter.ndu.edu/Portals/97/Doc…

Step-by-step horsepox study stokes dual-use controversy

cidrap.umn.edu/news-perspecti…
31. Synthetic horsepox viruses

the continuing debate about dual use research

journals.plos.org/plospathogens/…

Horsepox and the need for a new norm, more transparency, and stronger oversight for experiments that pose pandemic risks

journals.plos.org/plospathogens/…
32. Horsepox and Polio

unroll DARPA's secret Synthetic Polio Program

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More from @BillyBostickson

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GUESS WHO SAID THIS?

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You guessed right, i hope?

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She continued:

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@janeqiuchina About the film:

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Sebastian Castellio (1551)

To seek truth & to utter what one believes to be true can never be a crime. No one must be forced to accept a conviction

Conviction is free

One of the first proponents of religious toleration, freedom of conscience & thought

gospeltruth.net/zweig/heresy_c…Image
One of the great forgotten men of the Reformation.

One of the greatest minds in Geneva, a brilliant scholar and thinker.

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web.archive.org/web/2005030616…
The Plague in Geneva

At the same time, he was the only minister in Geneva during Calvin’s day to risk his life to assist the sick during the Black Plague.

The friend of Truth obeys not the multitude, but the Truth.”

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🧵Latest SAGO Report

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cdn.who.int/media/docs/def… x.com/R_H_Ebright/st…
2, Conflicts of Interest and Bias

Summed up by @R_H_Ebright

@R_H_Ebright 3. SAGO history and our struggle (January 2022)

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Jun 24
Bloody Hell, it's worse than even I imagined in my darkest nightmares about #scamdemia.

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Finally #scamdemia is waking up to the putrid stench of education for profit, ruined lives and academic credential inflation?

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Short 🧵on Primer & PCR Test issues (Pangolin Covs)

1. The key issue with pangolins and Pcovs is that:

It was discovered that the standard PCR tests used in 2020, and often still used, failed to actually detect many of the betacoronaviruses generously hosted by the pangolins.
2. Unless

The samples are retested with bespoke primers & more accurate PCR tests available now,

We will not know exactly whIch Pangolin (or bat) coronaviruses were hosted by Pangolins at WIV & elsewhere pre-pandemic.

see Nga et al., 2022 for details

frontiersin.org/journals/publi…Image
3. It is unlikely that anyone will do that now😒

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Jun 4
1. Tip of the Riceberg?

Not to mention wheat, barley and maize!

Read the disturbing criminal compaint here:

wlns.com/wp-content/upl… x.com/danaparish/sta…
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3. When asked why someone would put them into his bag, LIU stated that he did not know, and that maybe he had accidentally put the materials there. After further questioning, LIU acknowledged that the materials were different strains of the pathogen Fusarium graminearum
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