Thread by @nanopore

As 2022 draws to a close, we’ve been reflecting on some of the highlights (in no particular order) — you can read the full article here: bit.ly/3IfaKYc, or digest the thread below. Wishing you a happy new year and all the best for 2023! 1/23

The #cancer research community has made huge strides leveraging the unique attributes of #nanopore sequencing to characterise cancer at unprecedented resolution, unlocking previously hidden variation and accelerating research to support human health. nanoporetech.com/cancer-research 2/23

In May, @mdelledon took a group of scientists into the Gobi Desert to capture small mammals, including bats, to study their microbiome and they set up a genomics lab in a tent. They demonstrated the utility of the MinION in the harsh, off grid and sandy desert environment! 3/23

A complete human genome, at last! This year, the T2T Consortium finished the first truly complete 3.055 billion base pair (bp) sequence of a human genome, representing the largest improvement to the human reference genome since its initial release: nanoporetech.com/ncm-2022-human… 4/23

Two research studies broke new records this year. @euanashley’s team developed a WGS workflow that resulted in actionable characterisation of genetic disease in <8hrs. @danrdanny’s team developed a workflow to characterise inheritance of specific familial variants in <3hrs. 5/23

We rolled out the world’s most accessible high-yield sequencing device —– the palm sized P2 solo. We want this to open up genomics by enabling anyone, anywhere, access to human-scale genomic data at the highest levels of accuracy. nanoporetech.com/about-us/news/… 6/23

Roll out of Kit 14. Early access customers have shown >Q20 raw read (single molecule) accuracy with “Super Accuracy” base calling on default run conditions of 400 bps & Q30+ with duplex. We are now focused on supporting the broader communities to do the same. 7/23

Remora is the gold standard for methylation detection. Access precise whole genome #methylation detection from PCR-free #nanopore sequencing. We are also developing ways to look at more exotic modifications in all-contexts: nanoporetech.com/about-us/news/… 8/23

The aftereffects of lockdowns: our first hybrid London Calling moved the live audience from 600 in 2019 to >5,000. Register for London Calling 2023: londoncallingconf.co.uk/lc23 9/23

The first Mpox genomes were sequenced using @nanopore & shared openly to rapidly begin to understand this emerging outbreak. A protocol was then developed by @GSTTnhs to allow scientists to rapidly detect & identify unknown DNA and RNA viruses: nanoporetech.com/about-us/news/… 10/23

We successfully completed phase I of a DR-TB targeted study. This involved the development of a rapid end-to-end sequencing workflow to identify mutations associated with drug-resistance across the #TB #genome. Read here: nanoporetech.com/about-us/news/… 11/23

At this year’s European Federation for Immunogenetics, the team at @OmixonBio discussed their product development approach for rapid characterisation of #HLA using #nanopore sequencing. You can read more at the Omixon blog: omixon.com/nanotype-hla-a… 12/23

In April we were delighted to embark on the next phase & support @GenomicsEngland to sequence more #cancer genomes & to extend the application of our technology to more programmes to drive pioneering science & better clinical and health outcomes: genomicsengland.co.uk/initiatives/ca… 13/23

.@gordon_sanghera was interviewed for the Jazz Shaper podcast, hosted by @elliot_moss. He explained the real-world benefits of #nanopore technology, the rock n roll culture that inspired him to disrupt & what the future looks like. Listen here: planetradio.co.uk/jazz-fm/player/ 14/23

Several programmes launched this year with a goal of broadening genomics datasets. We are proud to work with these programmes, & strive through the design of our technology, pricing & service models to open up genomics to as many people as possible garvan.org.au/news-events/ne… 15/23

At this year’s Nanopore Community Meeting we spoke to some of our wonderful customers to hear about their work and what it was like at this year’s conference, check them out:

https://twitter.com/nanopore/status/1603102611552411648?s=20&t=0IYKej5YuV-BIf2eX5OFwg

16/23 #nanoporeconf

When 17-year-old @indeever_m pet angelfish died, he decided to sequence its genome – the first time this species has been sequenced. Read the full @newscientist article & wait for more news in 2023 on #nanopore sequencing as an educational tool newscientist.com/article/234351… 17/23

We are proud that entrepreneurs and researchers choose to take #nanopore sequencing close to environments, to better understand biodiversity. @Basecamp_Res are pioneering biodesign and responsible exploration of our planet's biodiversity: nanoporetech.com/resource-centr… 18/23

As we move towards our mission to enable the analysis of anything, by anyone, anywhere we love to see where our customers have got to this year! Check out the #anythinganyoneanywhere to see more!

https://twitter.com/DrMeaganDewar/status/1598100618543267840

19/23

@bbcworldservice spoke to @gordon_sanghera & @RosemaryDokos about ‘the DNA sequencing revolution’. They then discussed #nanopore sequencing in everything from #cancer research to #conservation with @LaraUrban42, @AnnaSchuh3 & Nino Susanto: bbc.co.uk/programmes/w3c… 20/23

The #Nanopore Community continues to increase the number of publications year on year. Some of our favourites include: Methylation profiling of cell-free DNA, Nanopore-only complete microbial genome assemblies & helping to track the evolving #SARSCoV2 epidemic in Africa. 21/23

We were pleased to announce a new collaboration with @10xGenomics to make single-cell and spatial full-length isoform transcript sequencing accessible to any laboratory. Learn more: nanoporetech.com/about-us/news/… 22/23

And finally, how many DNA bases have been sequenced on nanopore technology this year? Overall, the Community has sequenced enough DNA in 2022 to stretch for the length of: >26,800,000 MinIONs! Amazingly this would still only weigh the same as a few grains of salt. 23/23