CEO of Deep Piction, director at Helmholtz, LMU Prof. AI-based technologies for health. We’re🇩🇪🇹🇷🇭🇷🇨🇳🇮🇹🇮🇳🇸🇮🇴🇲🇨🇱🇭🇺🇧🇬🇺🇦🇷🇴🇦🇹🇹🇼🏳🌈
Jul 17 • 7 tweets • 3 min read
What if we could map every cell in the body, revealing its location and molecular identity? In this @NatureMethods perspective, we discuss the new era of 3D-omics by tissue clearing and AI, called Deep 3D Histology: PDF: 🧵👇🏼 rdcu.be/dNBe8 bit.ly/3WaD0QU 2- Tissue clearing techniques are evolving to enable imaging of intact specimens at cellular resolution. Learn how these methods, combined with light-sheet microscopy, are pushing the boundaries of 3D imaging from mouse embryos to entire human organs.
Apr 22 • 21 tweets • 6 min read
Very excited to share that our DELiVR method is now open access published @NatureMethods. We created a simple, brain-wide cell analysis deep learning tool, no coding needed! Fiji Plugin makes it accessible to all.
by @Dorie00 @Rami96614090 @moritz_negwer nature.com/articles/s4159… 2/n DELiVR is a robust deep-learning pipeline for whole-brain cell mapping. It operates through a user-friendly Fiji plugin. Re-trainable on custom data, DELiVR simplifies and streamlines brain cell analysis.
Aug 9, 2023 • 15 tweets • 4 min read
Exciting🥳 Our new study in Cell shows that our skull is a gateway for diagnosing & potentially treating brain diseases such as Alzheimer's & stroke. Imagine a future where a portable sensor on the skull can monitor brain health! @CellCellPress 🧵👇🏼with🔈 https://t.co/VrzVSra4lKcell.com/cell/fulltext/… 2/n Background: Neuroinflammation, the brain's immune system reaction common in disorders like stroke and dementia, can be like a fire damaging a house. Controlling it is tough as the brain isn't easily accessible.
May 23, 2023 • 17 tweets • 7 min read
Want to use deep learning for image analysis but lack the coding skills? We introduce DELiVR, a game-changer in brain-wide cell analysis. No coding required, our Fiji Plug-in does the magic. Hats off to @Dorie00, @Rami96614090, @moritz_negwer! 🧵👇🏼 1/n biorxiv.org/content/10.110…twitter.com/i/web/status/1…2. What makes DELiVR stand out? It's a robust deep-learning pipeline for mapping cFos+ cells in whole brains. Combining tissue clearing, light-sheet microscopy, VR annotation & deep learning, it comes in an easy-to-use FIJI plugin and docker container! biorxiv.org/content/10.110…
Apr 6, 2023 • 8 tweets • 3 min read
4/n Finding 1)
Along with many organs, we discovered spike accumulations in the skull marrow niches and recently discovered skull-meninges connections (SMCs), revealing a new route of pathogens into the brain. 5/n Finding 2)
Critically, we found the spike protein also in the skull bone marrow niches, and meninges of people who died from COVID-19.
Apr 6, 2023 • 4 tweets • 3 min read
😮~60% of us who had COVID still might have lingering viral spikes in our heads! Our new study reveals SARS-CoV-2 spike accumulation in the skull-meninges-brain axis & its implications in long COVID. By @zhouyi_rong@HongchengM@Sakethkapoor🔬🧠🦠🧵👇 biorxiv.org/content/10.110…2/n Summary:
We found SARS-CoV-2 spike protein in the skull-meninges-brain axis in mouse models and human post-mortem tissues long after their COVID, which was associated with vascular and inflammatory changes in the brain along with neuronal damage.
Feb 20, 2023 • 14 tweets • 8 min read
Imagine you could stain whole adult mice with standard IgG antibodies. What you couldn't do! We present wildDISCO technology for this🤗, creating the first whole body vascular, lymphatic, and nerve atlases. By @HongchengM@jieluo692biorxiv.org/content/10.110… 🧵👇🏼(turn on the sound)
2/n Summary:
wildDISCO is a new tissue chemistry enabling uniform staining of whole bodies with conventional (150 kDa size) IgG antibodies. It reveals the first whole mouse body cellular & structural maps similar to Allen Brain Atlas only that main them at the whole mouse level.
Dec 22, 2022 • 16 tweets • 12 min read
Spatial-omics goes 3D🤗! Out at Cell @CellCellPress 👉🏼 We developed DISCO-MS with @labs_mann, a spatial proteomics technology for specimens fully imaged in 3D. DISCO-MS is aided by robotics and enables the study of diseases at their early stages. cell.com/cell/fulltext/…🧵👇
Summary:
DISCO-MS is a spatial proteomics technology: it enables proteomics analysis of cleared tissues imaged in 3D. DISCO-MS is aided by AI and robotics and yields proteome similar to fresh or fixed samples.
Have questions? Add them below👇 we will answer📖
Feb 13, 2020 • 13 tweets • 7 min read
We are very excited to share our SHANEL method making the intact human organs transparent, just published at Cell. cell.com/cell/fulltext/… by @shan_heather et al.
See short tweetorial for details: #clearing#imaging#3D#deeplearning#AI@CellCellPress
Mapping the human brain or other organs is a crucial step forward deciphering how they function in health and disease.
Dec 31, 2018 • 12 tweets • 6 min read
I am excited to share our publication of vDISCO technology: a whole mouse body clearing & imaging method nature.com/articles/s4159…. The thread below presents details of vDISCO and its biological findings. @NatureNeuro@nature. Happy New Year all! 1/12
2 years ago, we published uDISCO to clear and image intact adult mouse body with light-sheet microscopy. However, neuronal details were mostly invisible through intact bones, skin and highly autofluorescent muscles. nature.com/articles/nmeth…. 2/12