1/n The #IPFCellAtlas is finally out! We profiled 312,928 cells from 32 IPF, 28 controls, 18 COPD lungs using @10xGenomics #scRNAseq & identified previously unidentified populations of epithelial & endothelial cells enriched in IPF #CureIPF advances.sciencemag.org/content/6/28/e… 
2/n Among epithelial cells, we identified cells DISTINCT from ANY epithelial cells previously described in human lungs. These aberrant basaloid cells are at the edge of myofibroblast foci & express fibrotic molecules & regulators #IPFCellAtlas #CureIPF advances.sciencemag.org/content/6/28/e…
3/n Among enodthelial cells we discovered a previously undescribed population of COL15A1+ CD31+ cells. They are usually restricted to the airway but in IPF are found in the lung parenchyma - what are they doing there? #IPFCellAtlas #scRNAseq #CureIPF advances.sciencemag.org/content/6/28/e…
4/n We are not fibroblast experts - but our archetype analysis suggests source of IPF 'myofibrolasts' is a 'myofibroblast like' population in the normal lung, also IPF invasive fibroblasts are not myofibroblasts - true? #IPFCellAtlas #scRNAseq #CureIPF advances.sciencemag.org/content/6/28/e…
5/n We confirmed the dominance of profibrotic macrophages among immune cells in IPF lungs as described before & applied archetype analysis to classical monocytes, profibrotic IPF macrophages & control-enriched macrophages #IPFCellAtlas #scRNAseq #CureIPF advances.sciencemag.org/content/6/28/e…
6/n In a preview of future work we implemented a gene regulatory network inference approach to our #scRNAseq data because because we need to understand how the organ is disregulated to be able to find ways to reprogram it #CureIPF #IPFCellAtlas advances.sciencemag.org/content/6/28/e…
7/n In our supplemenraty data we provide externsive validations, assessment of batch effects, cell cycle and other effects, much done by our superb team of collaborators at @Numedii - dig in! advances.sciencemag.org/content/suppl/… #datasharing #CureIPF #IPFCellAtlas
advances.sciencemag.org/content/6/28/e…
advances.sciencemag.org/content/6/28/e…
8/n And of course all our data is publicly available on the Gene Expression Omnibus (GEO) (GSE136831), and the results can be explored through the IPF Cell Atlas Data Mining portal ipfcellatlas.com #CureIPF #datasharing #IPFCellAtlas p2med.shinyapps.io/IPFCellAtlas/
9/n What's even more exciting is that there are TWO #scRNAseq #IPFCellAtlas papers in same issue @ScienceAdvances - from us & from the super group of @JKropski & @NeBanovich - together we provide data from 450,000 cells & >100 human lungs #CureIPF advances.sciencemag.org/content/6/28/e…
