Congrats Nick github.com/Nick-Eagles for your @biorxivpreprint first pre-print! 🙌🏽

SPEAQeasy is our @nextflowio implementation of the #RNAseq processing pipeline that produces @Bioconductor-friendly #rstats objects that we use at @LieberInstitute

📜 biorxiv.org/content/10.110…
This project started a few years ago with Emily Burke linkedin.com/in/emilyeburke/ @BadoiPhan @andrewejaffe with whom we wrote a version specific to our HPC 🖥️ (JHPCE)

github.com/LieberInstitut…

Then @Jake_Aaron96 Israel Aguilar et al from @wintergenomics rewrote using it @nextflowio
Nick continued developing it with input from @sudo_BreeB and tests from colleagues

Nick also wrote the documentation website research.libd.org/SPEAQeasy/

Then @JoshStolz2 @lahuuki helped show the outputs can be analyzed with @Bioconductor

research.libd.org/SPEAQeasy-exam…
We've used these #RNAseq outputs extensively in previous projects with @andrewejaffe et al

Some examples:

doi.org/10.1016/j.neur…
doi.org/10.1038/s41467…

Now our colleagues can use it too without having to tweak all the processing steps ^^
Next up, Nick & @JoshStolz2 will teach a workshop on how to use it at #EuroBioc2020 next week

eurobioc2020.bioconductor.org/conference_sch…

research.libd.org/SPEAQeasyWorks…

I also bet that you'll see Nick's name around more frequently. He's just getting started ;)

The first paper is always the hardest 😤

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More from @lcolladotor

29 Feb
🔥off the press! 👀 our @biorxivpreprint on human 🧠brain @LieberInstitute spatial 🌌🔬transcriptomics data 🧬using Visium @10xGenomics🎉#spatialLIBD

🔍spatial.libd.org/spatialLIBD/
👩🏾‍💻github.com/LieberInstitut…
📚research.libd.org/spatialLIBD/
📦github.com/LieberInstitut…

biorxiv.org/content/10.110…
Our study shows 12 samples from 3 human brain DLPFC neuronal layers + white matter with @10xGenomics's Visium platform. Features📜:

* Largest so far
* Harder to cluster than the example mouse data
* Data is all public
* @Bioconductor #rstats📦
* #shiny web app

2/7 #spatialLIBD
* Assessment of known markers
* Identification of layer-specific markers
* smFISH validation✅
* spatial registration of snRNA-seq data
* Layer-enrichment of neurodevelopmental and neuropsychiatric gene sets
* Data-driven layer-enriched clustering in the DLPFC

3/7 #spatialLIBD
Read 7 tweets

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