Thread by @Matt_Snelson

Latest paper is up! We fed mice high protein diets for 24 weeks and measured the #microbiome 🦠, #kidney function and #gut leakiness. Here's what we found: onlinelibrary.wiley.com/doi/abs/10.100… @sihmin_tan @MelindaCoughlan @MonashDiabetes

1) high protein diets alter the microbiome...but what's interesting is when we looked at the predicted metagenomes (using picrust2) we saw⬇️BCAA synthesis and⬆️urea cycle pathway.

2) the high protein diet caused inflammation and intestinal permeability:
⬆️ plasma endotoxin
⬆️ plasma MCP1
⬆️ TNFα expression in jejunum and ileum
⬇️ occludin expression in jejunum

3) and the high protein diets damaged the kidneys:
⬆️ albuminuria
⬆️ blood urea nitrogen
⬆️ urinary KIM-1
⬆️ renal TGFβ

4) Also I'm super happy to say the code used for the microbial bioinformatics is available online (it's been a fun learning journey with #rmarkdown and #githubpages):
💻 mattsnelson.github.io/hpd_16S/