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Billy Bostickson 🏴👁&👁 🆓 Profile picture
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7 Mar, 4 tweets, 2 min read
(A and B Sequence & structural properties of insert PRRA (D) Sequence similarity between close neighborhood of PRRA insert, neurotoxin motifs Y674QTQTNSPRRAR685, homologous to Ophiophagus (cobra) & Bungarus genera & 3 RABV strains & HIV-1 gp120 superantigenic motif in last row
Study on Ribonuclease From Snake Venom of Zhejiang Cobra (Naja naja atra)
ZHANG Gon-jin
Wuhan Institute of Virology,Academia Sinica
zoores.ac.cn/article/id/1281
SdPI, the first functionally characterized Kunitz-type trypsin inhibitor from scorpion venom.
europepmc.org/article/pmc/32…

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More from @BillyBostickson

Billy Bostickson 🏴👁&👁 🆓 Profile picture
7 Mar
Compilation Thread on ORF8 ORF8 or Fate?
1. ZLS (2018):
"Considering the variability of orf8 in bats, civets and humans, investigating the function of orf8 is a priority, particularly the contribution of these different variants to viral pathogenesis."
2. ORF8 or Fate?
"In addition to receptor binding, proteolytic cleavage of S & potentially other mutations that affect virion & trimer stability may be important for virus transmissibility in different hosts, & these factors need to be studied further."
nature.com/articles/s4157…
3. ORF8 or Fate?
Overwhelmingly these viruses had mutations or
29 deletions in ORF8 - associated with reduced replicative fitness of the virus.. may be associated with host adaptation
via @CarltheChippy
382-nt deletion during early evolution of SARS-CoV
biorxiv.org/content/10.110…
Read 30 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
5 Mar
1. Within-Run Cross-Contamination in Deep Sequencing Applications on the Illumina MiSeq

croiconference.org/abstract/withi…
2. Common Contaminants in NGS
Until an effective strategy is found to eliminate contaminants from sequencing runs, novel sequences detected in such runs should be scrutinized by qPCR analysis to ensure they originate from specimens rather than the lab
journals.plos.org/plosone/articl…
3. From trash to treasure: detecting unexpected contamination in unmapped NGS data
bmcbioinformatics.biomedcentral.com/articles/10.11…

Here we propose DecontaMiner, a tool to unravel the presence of contaminating sequences among the unmapped reads
Read 7 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
5 Mar
@Daoyu15 @franciscodeasis @spectropan @ResearchGate @TheSeeker268 STRANGE TO SEE THIS IN Wuhan Hu-1
Wuhan seafood market pneumonia virus isolate Wuhan-Hu-1
Clostridium_difficile
Bat coronavirus BM48-31/BGR/2008
Human coronavirus 229E
@Daoyu15 @franciscodeasis @spectropan @ResearchGate @TheSeeker268 A Bulgarian Bat Virus mentioned in Lam et al:
Identification of 2019-nCoV related coronaviruses in Malayan pangolins in southern China
biorxiv.org/content/10.110…
@Daoyu15 @franciscodeasis @spectropan @ResearchGate @TheSeeker268 Bat coronavirus BM48-31/BGR/2008

Drosten

ncbi.nlm.nih.gov/nuccore/NC_014…
and
ncbi.nlm.nih.gov/Taxonomy/Brows…
Read 10 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
4 Mar
1. I hate Tax Returns but these EcoDeath Alliance ones may be interesting to peruse:
2001: projects.propublica.org/nonprofits/dis…
2002:
projects.propublica.org/nonprofits/dis…
2003:
projects.propublica.org/nonprofits/dis…
2005:
projects.propublica.org/nonprofits/dis…
2006:
projects.propublica.org/nonprofits/dis…
2007:
projects.propublica.org/nonprofits/dis…
2. EcoDeath Alliance Tax Returns
2008:
projects.propublica.org/nonprofits/dis…
2009:
projects.propublica.org/nonprofits/dis…
2010:
projects.propublica.org/nonprofits/dis…
2011:
projects.propublica.org/nonprofits/dis…
2012:
projects.propublica.org/nonprofits/dis…
2013:
projects.propublica.org/nonprofits/dis…
2014:
projects.propublica.org/nonprofits/dis…
3. EcoDeath Alliance Tax Returns 2015 - 2020
2015:
projects.propublica.org/nonprofits/dis…
2016 Audit:
projects.propublica.org/nonprofits/dis…
2016 Full Filing:
projects.propublica.org/nonprofits/org…
Form 990" for fiscal year ending June 2016
projects.propublica.org/nonprofits/org…
Read 8 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
28 Feb
AN ASSESSMENT OF CHINA’S BIOLOGICAL WARFARE CAPABILITIES AND NEED FOR GLOBAL APPROACH TO BIO-SECURITY Part 1 (Indian Analysis, May 2020) researchgate.net/publication/34…
Another:
japan-forward.com/biological-wea…
China's Biological Timeline
media.nti.org/pdfs/china_bio…
Read 5 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
28 Feb
Same covid-19 mutations are appearing in different places, which may or may not be strange....
41,000 mutations documented in 880 lineages.
Most in spike protein
What explains it?
Read 18 tweets

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