A 🧵on our new work on preclinical #FaecalMicrobiomeTransplantation studies!
A while back, I began to plan my own #FMT study.
Turns out, the literature is wildly varied but mainly it is pretty difficult to find a protocol that has all the nitty gritty detail you need.
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A while back, I began to plan my own #FMT study.
Turns out, the literature is wildly varied but mainly it is pretty difficult to find a protocol that has all the nitty gritty detail you need.
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So me and my team (@kate_secombe @GhanyahA @CourtneySubra11 @JacquiScott0 @YsabellaVan @JoanneBowen_UoA) and some incredible collaborators (@jfcryan @CassGheorghe @caitlincowanPhD @Matt_Snelson @UCCpsychiatry) decided to do something about it!
@Pharmabiotic @UniofAdelaide
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@Pharmabiotic @UniofAdelaide
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We systematically reviewed all #FMT studies (in mice) in the past decade, extracting all the methodological details they used/reported.
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A whooping 92.5% of the studies we reviewed reported a "positive" effect of the #FMT intervention.
This is consistent with findings in @CellCellPress from @Pharmabiotic's Jens Walter (95%).
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doi.org/10.1016/j.cell…
This is consistent with findings in @CellCellPress from @Pharmabiotic's Jens Walter (95%).
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doi.org/10.1016/j.cell…
Walter and colleagues posited this number implausible (even in light of publication bias), advocating for more rigorous approaches to #microbiome science when inferring causality.
So thats what we did!
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So thats what we did!
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In our #systematic review, there were some major omissions in FMT methods...
92% failed to report on the use of anaerobic conditions, 50% did not report on #FMT storage, 25% DID NOT REPORT ON THE METHOD OR VOLUME OF FMT ADMINISTERED!
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92% failed to report on the use of anaerobic conditions, 50% did not report on #FMT storage, 25% DID NOT REPORT ON THE METHOD OR VOLUME OF FMT ADMINISTERED!
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22% did not check whether their FMT had colonised the recipient
88% did not perform any QC on their FMT product (e.g. viability)
40% did not describe what their control animals received ...
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88% did not perform any QC on their FMT product (e.g. viability)
40% did not describe what their control animals received ...
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We decided there was a clear need to provide guidance on what information is needed for studies reporting on preclinical #FMT, and developed the GRAFT Framework!
A great tool for people planning, reporting and reviewing FMT studies!
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A great tool for people planning, reporting and reviewing FMT studies!
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Better still, we made a simple and easy to use check-list! Want a copy? Just slide into my DMs or email me Hannah.wardill@adelaide.edu.au - I would be happy to share!
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Alternatively, check out the full paper in @tandfonline, available now!
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tandfonline.com/doi/full/10.10…
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tandfonline.com/doi/full/10.10…
If you are an editor and think these guidelines would be useful to improve the rigour of papers submitted to your journal, I would love to connect!
@MicrobeTweets @MRC_microbiome @cellhostmicrobe @MicrobioticaLtd @FZMarques @Pharma4d @NatureRevMicro @WeizmannScience @EranElinav
@MicrobeTweets @MRC_microbiome @cellhostmicrobe @MicrobioticaLtd @FZMarques @Pharma4d @NatureRevMicro @WeizmannScience @EranElinav
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