Picking up where @rita_rebollo left, we spent a couple of years annotating and analyzing the rest of the rice #weevil #genome (ok, the 15% left after removing repeats)! All info presented here is on the huge Additional file 2 in @BMCBiology doi.org/10.1186/s12915… 1/n
@VargasChavezC and @gabaldonlab performed a comprehensive #phylogenomic analysis of the rice #weevil and found that this insect has a high gene expansion rate when compared to other beetles. 2/n Image
With the invaluable help of the SymT group from @INRAE_UMR_BF2I we have also reconstructed the #metabolic pathways from both #symbiotic partners. Specific losses of enzymes in the #weevil genome are limited while its #endosymbiont has suffered much more degradation. 3/n
But both host and symbiont collaborate to synthesize some essential vitamins! 4/n Image
@VargasChavezC and I annotated lots of gene families including digestive enzymes, cuticle proteins, detoxification and #insecticide resistance related genes. 5/n
And thanks to the help of our collaborators we had the opportunity to annotate developmental genes (@CallaertsLab from @KU_Leuven) and odorant receptors (@EmmaJoly8, N. Montagné et @CamilleBurgh from @iEESParis) 6/n
The analysis of #immunity related genes was done at @INRAE_UMR_BF2I. @cmonegat and @AnnaZaidmanRemy coordinated the work of our former #PhD students @VargasChavezC @Justin_Maire @flmasson and A. Vigneron. 7/n
They found that despite the interaction with S. pierantonius, the rice #weevil genome encodes the canonical genes involved in the 3 main #antimicrobial pathways Toll, Imd, and JAK-STAT. 8/n Image
The Imd pathway is not only present but it is also functional and has evolved molecular features necessary for endosymbiont control and host immune homeostasis. As seen in doi.org/10.1186/s40168… and doi.org/10.1073/pnas.1… 9/n
On #epigenetics @rita_rebollo, A. Vallier, @GAUTIERRichard, @VargasChavezC & co found only DNMT1 & 2 (not 3) and around 1% of #CpG methylation. They also annotated the #piRNA genes, and albeit the presence of SIWI, most piRNA-related genes are overexpressed in germ vs soma 10/n
I would like to end this thread by thanking A. Heddi and @amparo_latorre for integrating me in the middle of their journey and for their strong support. 11/n
A big thanks also to @VargasChavezC for his incredible contribution, so challenging given the complexity of the genome. 12/n
Finally, I thank @rita_rebollo @clementgoubert and @TreepVieira for the time spent to analyze this genome full of repeats. You, guys, can be proud of what you have done here! 13/13

• • •

Missing some Tweet in this thread? You can try to force a refresh

Keep Current with Nicolas Parisot

Nicolas Parisot Profile picture

Stay in touch and get notified when new unrolls are available from this author!

Read all threads

This Thread may be Removed Anytime!


Twitter may remove this content at anytime! Save it as PDF for later use!

Try unrolling a thread yourself!

how to unroll video
  1. Follow @ThreadReaderApp to mention us!

  2. From a Twitter thread mention us with a keyword "unroll"
@threadreaderapp unroll

Practice here first or read more on our help page!

Did Thread Reader help you today?

Support us! We are indie developers!

This site is made by just two indie developers on a laptop doing marketing, support and development! Read more about the story.

Become a Premium Member ($3/month or $30/year) and get exclusive features!

Become Premium

Too expensive? Make a small donation by buying us coffee ($5) or help with server cost ($10)

Donate via Paypal

Or Donate anonymously using crypto!


0xfe58350B80634f60Fa6Dc149a72b4DFbc17D341E copy


3ATGMxNzCUFzxpMCHL5sWSt4DVtS8UqXpi copy

Thank you for your support!

Follow Us on Twitter!