I’m incredibly excited to share my postdoc work in the @FujimoriLabUCSF, in collaboration with @fraser_lab, @fiddle, & the Takeuchi-Tomita lab!!!! We usebiochemical & structural approaches to investigate mitoribosomal inhibition by antibiotics 🧵 1/n doi.org/10.1101/2024.0… Before I get too far, I want to make sure all the authors get their credit! It was truly a cross-country and cross-continent team effort, and I'm so happy I can now tell you all about it! 2/n

I don't know if these would be helpful to anyone, but I found a bunch of protein crystallization diagram graphics I'd made a long time ago that were embarrassingly ugly & and "refreshed" them - you can find them all here: bit.ly/crystallizingp…

Say you want to study how a protein works... What can you do? A thread on recombinant protein expression and purification (with links to more) 1/ The first step's molecular cloning, where you take the genetic instructions for making that protein (cDNA) & stick them into another piece of DNA (such as a plasmid) that you can use as a "vector" (vehicle) to get the instructions into expression cells bit.ly/molecularcloni… 2/

when planning experiments, I make a sort of "summary" sheet beforehand listing reaction conditions, etc. for reference later (such as when I'm exhausted from the experiments!) & include a "goals" section so I always keep in mind *why* I'm doing all that hard work #labtips n/n I have a "storyboard" Adobe Illustrator artboard where I lay out my key figures & how they tell the story of my research - what's the point of each? anything missing? and wow - you've really done a lot! (note: free alternatives to Illustrator are Inkscape & GIMP) #labtips n/n

Just in case you need some splice in your life... 1/8: gene to protein overview 2/8: pre-mRNA to mRNA

Spectroscopy allows us to spy on molecules invisible to the naked eye. Through a cuvette the spectrophotometer allows us to peer and measure concentrations with the help of Beer! 👉 bit.ly/2N4nzXE
#366DaysOfScience #biochemistry #scicomm #realtimechem The Beer Lambert law (Beer’s law) allows you to convert between concentration (c) of a dissolved thing (solute) & absorbance of light. Don't have a lot - use a NanoDrop so the UV-Vis fun don't stop! bit.ly/beerslawexplai…
#366DaysOfScience #biochemistry #scicomm #realtimechem

After a jam-packed 2 days at #SciOut18🌪(thx @ASBMB & @rockedu_!) it was time to reflect! 🤔 Reflection is so important, but it can be hard to find ⏰ to really just think! 💭 Some of my thoughts 👉 instagram.com/p/BokNO_NhihF/
#365DaysOfScience #biochemistry #scicomm #sciout #science What’s inside? 🧐 It’s FORMAMIDE! 🤗 Formally removing RNA’s form with formamide! 🤓 FORMAMIDE in loading buffer breaks up & replaces the bonds between bases holding RNA's shape together 👇
instagram.com/p/BomwR32B4MX/
#365DaysOfScience #biochemistry #scicomm #lifeinthelab #science