Discover and read the best of Twitter Threads about #relion3
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So excited to finally share this work - structure of Cx46/50 gap junction intercellular channels in a dynamic lipid environment, resolved at 1.9 Å by #CryoEM! With some MD-simulation to make it dance ;) Check it out on BioRxiv: shorturl.at/iEW34
Lead by an extraordinary team of grad/undergrad students: Jonny Flores (@CryoEMJonny), Bassam Haddad (@BassBioPhys), Kim Dolan and Janette Myers – and our wonderful collaborators Craig Yoshioka (@craigky), Jeremy Copperman and Dan Zuckerman @psu_research, @OHSUSOM
We see a stunning effect of Cx46/50 on the local lipid environment - effectively inducing a phase separation (to the gel state) that is specific to the extracellular lipid leaflet of the two opposed membranes
1.62 Å from the first post-installation test dataset of our Krios @UTokyo_News_en. Thanks to the superpowers of #Relion3, @SjorsScheres @biochem_fan. Also thanks to a great apoferritin prep by Yanagisawa-san from @mkikkawa lab. 
Dataset details:
- Falcon 3EC, subframe alignment
- x155k, NanoProbe
- Spot 6
- Pixel size 0.52 A
- Exposure rate 0.74 e/pix/s
- Total dose 50 e/A2
- Fractions (frames) 59
- Exp. time 18.21 s
- Defocus range 0.3 - 1.2 um
- Micrographs 1,647 total, 1,297 used
- 190k picked picls
- Falcon 3EC, subframe alignment
- x155k, NanoProbe
- Spot 6
- Pixel size 0.52 A
- Exposure rate 0.74 e/pix/s
- Total dose 50 e/A2
- Fractions (frames) 59
- Exp. time 18.21 s
- Defocus range 0.3 - 1.2 um
- Micrographs 1,647 total, 1,297 used
- 190k picked picls
Trefoil (threefold astigmatism) visible in the phase difference plot from #Relion3 CtfRefine. This makes me believe that in the future higher order aberrations will be correctable in software .
