Discover and read the best of Twitter Threads about #cryoem

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1/9 #cryoEMtweetorial - using tilts in SPA
While visiting some friends this weekend, I was introduced to the idea of the #tweetorial which seems to be popular in the medical field. I figured it would be useful too in #cryoEM, so here it is, the first of the series.
Firstly, if you hadn't guessed by my banner, I think tilting the specimen provides useful information. In "Addressing Preferred Specimen Orientation in Single-Particle Cryo-EM through " @DmitryLyumkis @bcarra2
show a great example.…
One of the (many) complications in analyzing an image of a tilted specimen, is the defocus gradient perpendicular to the tilt axis. It turns out with a few practical considerations, it is easy to use #cisTEM right out of the box to work with your tilted data.
Read 10 tweets
Hello, I am Edna Matta. I was born in #Ibague, known as #Colombia’s #CityofMusic. A beautiful place near snow-covered volcanos (Ruiz, Santa Isabel and Tolima), large expanses of paramo forest, lakes and streams where a diverse array of animal and plant species coexist
My story starts with #powerful, #strong #women in my #family. I was the third of 4 children and my mom #firstMentor had to raise us practically alone, since my father had to work in a different city, 20h away by car
My mom is a very #strong #woman that taught me #values, to #workhard and to be #self-sufficient #StrongWomen #StrongFamily
@IWS_Network #VoicesIWS /1
Read 36 tweets
@SjorsScheres @RubinsteinJohn @AlexisRohou @cjrlab @structurabio You can't change it retroactively, but you can change it for future versions. You can also release it under different licenses in parallel, e. g. grant some company the right to make a closed-source derivative. All of this requires clean separation of parts you haven't authored.
@SjorsScheres @RubinsteinJohn @AlexisRohou @cjrlab @structurabio I imagine most GUIs in #cryoEM software could be made non-GPL without stepping on anyone's toes. As the field matures, ease of use becomes an equally important feature as the computational back end. Maybe something to consider when thinking about competitive advantages.
Read 3 tweets
Second presentation on #ResearchMisconduct Alexander Wlodawer looking in detail at careless and rare fake structures in Crystallography and CryEM @FEBSnews
Wlodawer: several examples of structures deposited but not published, even many years later #ProteinStructure
Wlodawer: most notorious case of outright #Fabrication H.M. Krishna Murthy from University of Alabama…
Read 10 tweets

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