Thread by @DrJohnB2

(1/n) The #AstraZeneca #CovidVaccine is contaminated with human proteins/peptides: researchsquare.com/article/rs-477…
More than 1000 proteins/peptides were found.
They are most likely from the human embryonic kidney 293 cells (HEK293) used for production of the vaccine.

(2/n) Results of SDS polyacrylamide gel electrophoresis (SDS-PAGE) followed by silver staining:
High contamination with human proteins/peptides.

(3/n) The amount of contamination with uman proteins/peptides varies between lots!

(4/n) The most abundant protein in the #AstraZeneca vaccine is a human protein: the heat shock proteine 90 -beta (HSP 90-beta)!

(5/n) "Extracellular HSPs modulate innate and adaptive immune-responses, can exacerbate pre-existing inflammatory condition, have been associated with autoimmunity and can even become target auf auto-immune responses themselves"

(6/n) "Several of the HSPs also have ATPase activity and might directly be involved in the activation of platelets ..."

(7/n) "The often-observed strong clinical reaction one or two days after vaccination is likely associated with the detected protein impurities. A linkage to later immune-related adverse events is also conceivable.

(8/n) The fact that vaccines often contain contaminations with animal and/or human proteins as well as nucleic acids is well documented in peer-reviewed scientific publications (but not well-known to the general public).

(9/n) For example, analysis of the Pandemrix and Arepandrix vaccines (bird flu) showed that there was also considerable contamination with foreign proteins: sciencedirect.com/science/articl….

(10/n) 293 different chicken proteins were found in the Arepandrix vaccine (sciencedirect.com/science/articl…)!
Both vaccines were associated with the occurrence of narcolepsy
(link.springer.com/article/10.100…).

(11/n) In addition, the "heart fatty acid binding protein" was also found - it plays a role in the development of Alzheimer's disease (pubmed.ncbi.nlm.nih.gov/24088526).

(12/n) The fact that vaccine sera are often contaminated with foreign DNA has been shown by several investigations. Contamination of vaccine sera with human DNA has been found (hopkinsvaccine.org/components-Exc…) without any consequences for the manufacturer or restrictions on use.

(13/n) For example, fetal human DNA is contained in the vaccines Flublok (influenza), Flublock Quad (influenza), Flucelvax Quad (influenza), Ixiaro (Japanese encephalitis), ProQad (MMR), RotaTey (rotavirus), Shingrix (zoster), Vaqta (HepA), Varivax (varicella), Zostavax (zoster)

(14/n) DNA of porcine Circivurus 1 has been detected in the Rotarix vaccine (USA) (pubmed.ncbi.nlm.nih.gov/20375174/).

(15/n) The Corvelva team studied common pediatric vaccines. Result: Proprix Tetra contained 1.7-3.7 µg of foreign DNA (chicken DNA & DNA from a human fetus). This vaccine contains more than 100 times more than legally allowed (WHO; limit: 0.01 µg)!

(16/n) Gardasil 9 (HPV) is heavily contaminated by bacterial DNA ("54 per cent of the total DNA of the vaccine was bacterial"). The Corvelva team's analyses can be viewed here: tinyurl.com/545r8v3c

(17/n) Finally, one should also investigate how high the contamination with other viruses, bacteria and phages is. Every cell culture is usually contaminated with viruses, bacteria and phages (despite the use of antibiotics).

(18/n) The Simian virus 40 (SV40) was found in some cell cultures. It is also likely to be present in the HEK 293 cell line used by AstraZeneca. SV40 contamination was once a major issue in polio vaccination: cancerres.aacrjournals.org/content/65/22/…

(19/n) The work of T. Deisher ist also relevant in this context: "Vaccines manufactured in human fetal cell lines contain unacceptably high levels of fetal DNA fragment contaminants." pubmed.ncbi.nlm.nih.gov/26103708/
PDF: tinyurl.com/yz3x8cw7

(20/n) Human fetal DNA in Meruvax (rubella virus vaccine) and Havrix (hepatitis A vaccine).
Source: tinyurl.com/yz3x8cw7

(21/n) The results of Krutzke et al. (researchsquare.com/article/rs-477…) about the contaiminations in the AstraZeneca vaccine got confirmed by Schultz et al. nejm.org/doi/full/10.10…
High contamination with human proteins/peptides.
The figure is in the Supplementary Materials.

(22/n) Two studies (researchsquare.com/article/rs-477… and nejm.org/doi/full/10.10…), the same finding: The #AstraZeneca vaccine contains a lot of proteins/peptides that should not be present

(23/n) Krutzke et al. found "peptides from more than 1000 different human proteins" in the vaccine.
Schultz et al. (nejm.org/doi/full/10.10…) now reports finding 1856-1939 proteins (!) with 21 poteins from the Adenovirus and 1 from #SARSCoV2, i.e. 1834-1917 human proteins!

(24/n) 43-60% of the proteins in the #AstraZeneca vaccine are human proteins (from the HEK-293 cell line, i.e. human embryonic kidney cells). The presence of human proteins in the vaccine is a contamination.

(25/n) 11-24 of these human proteins were present in the vaccine in a concentration similar to the main ingredient (the adenovirus)!
Most of them were cell membrane proteins.
For sure, the immune system will react to these human proteins when one gets them injected.