#Viral_Capsids

A look at viral capsids.
1/ The capsid is a strong protein structure that encloses and protects the viral genome. The most basic viruses will use a single protein produced many times to build the capsid. The more complex viruses will use multiple proteins to build their capsid structure.
2/ The basic structure of the viral capsid comes in 3 basic designs. They are Icosahedral, Helical and Complex.
3/ The Helical symmetry of capsids takes 1 protein and links them together into a very long string. That string is then wrapped into a helical structure like a tube.
4/ An example of a helical capsid virus is Ebola. This virus actually looks like a worm because of its helical capsid.
5/ The Icosahedral capsid is made up of triangles to form a sphere like shape. If you ever seen a 20 sided die from the D&D game, that is a perfect example of an icosahedral shape.
6/ The basic unit of the icosahedral capsid is a single viral protein often designated as Viral Protein 1 (VP1). Some viruses will use multiple proteins to form its basic triangle structure and will designate them VP1, VP2 and VP3.
7/ This small structure is the most basic building block of the icosahedral structure. When 3 of these proteins come together, they form the basic triangle of the icosahedral capsid. The single triangle is called a facet.
8/ The most basic virus is made up of only 20 facets. This is also called its T number. A T-1 virus would have 20 facets. A T-2 virus would have 40 facets and a T-3 virus would have 60 facets.
9/ Since each facet is made up of the 3 basic proteins, the T number that represents the facets gets multiplied by 3 to find out the total proteins in the capsid. For example a T-1 capsid has 20 facets with 3 proteins per facet = 60 total proteins.
10/ A T-3 capsid would have 20 x 3 facets or 60 total facets with 3 proteins to make up every facet. That would be 60 x 3 = 180 total proteins.
11/ When a capsid falls into icosahedral symmetry, it will have specific shapes if forms. There are 2 fold, 3 fold and 5 fold axes in each 20 facet capsid.
12/ As the virus capsid gets bigger into the T scale, the number of 5 fold axes increase so that the capsid goes from a basic of triangle faces to pentagon shaped faces made up of these 5 fold triangles shown below.
13/ In a large enough capsid, you will begin to see 6 fold axes of symmetry. They begin to look like the pattern on a soccer ball.
14/ The last Capsid is the Complex which is made up of both helical and icosahedral parts. The classic example of this type of virus is the bacteriophage.
15/ The head of the bacteriophage is made up of a basic icosahedral shape which is attached to a long tube that is made up of a helical structure. These do not harm humans and prey on bacteria hence their names.
16/ The Adeno Associated Virus (AAV) is a basic T-1 icosahedral capsid. Its made up of 20 facets. The most basic uses only 1 protein VP1 with 60 copies to make up the capsid. Others will use 3 different proteins with VP1, VP2 and VP3 in 20 copies of each to build the capsid.
17/ The AAV capsid is the smallest you can find. It only holds about 4,500 bases (4.5kb) of RNA or DNA genetic material.

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More from @Biotech2k1

25 Sep
#Viral_Genomes

A look at the Baltimore scale for viral genomes.
1/ There are over 21 families of viruses. Each of these families will have many strains of viruses within them. Just the Herpes virus has 8 different strains within its family. Others like Flavivirus has strains like Dengue, Zika, West Nile and Yellow Fever.
2/ Other then classifying viruses by their family, we can classify them by their genetic makeup. There are 7 different classes of viral genomes. They are classified using the Baltimore scale shown below.
Read 20 tweets
25 Sep
Been Working on my Science Categories:

Pathways 20% max
Synthetic Lethality 15% max
CRISPR 15% max
Protein Degraders 15% max
Cell Therapies 15% max
Delivery 15% max
Max 95% with 5% cash
I think come out to a good diversification. It would never get that low on cash as I plan a few transitions. I am splitting up Pathways and Synthetic Lethality which is kind of a judgement call since they highly overlap.
Pathways:
1 $BPMC max 5% = 3.3%
2. $TPTX max 5% = 3.3%
3. $RVMD max 3% = 2%
4. $ERAS max 3% = .67%
5. $RLAY max 3% = 2%
Max 19%
Read 8 tweets
25 Sep
Here are my Watch Lists:

These are the companies on my lists for when the bubble pops in biotech. Most of them are grossly overvalued, but awesome companies.
Pathways:

1. $BPMC - fair value
2. $MRTX - expensive
3. $TPTX - fair value
4. $SDGR - very expensive
5. $RVMD - very expensive
6. $ERAS - very expensive
7. $RLAY - bubbly
8. $RPTX - very expensive
Protein Degraders:

1. $ARVN - bubbly
2. $KYMR - bubbly
3. $CCCC - bubbly
4. $GLUE - very expensive
Read 7 tweets
24 Sep
#Cell_Therapies iPSC

A look at induced Pluripotent Stem Cells.
1/ This has been a huge love of mine for years. The engineering of cells to create cancer therapies. The induced Pluripotent Stem Cells (iPSC) can revolutionize the way we create CAR-T or CAR-NK cells for cancer treatments.
2/ The process of the iPSC can be automated, duplicated and consistent as a source of low cost cell therapies. So how does this work?
Read 15 tweets
24 Sep
My Exit Strategy Updated:

Not at my trading laptop so all are estimates. I plan to fade all rallies in biotech back to old high as value for the $XBI is only $112.
Pathways:

$BPMC 3.34% will keep my 2.02% paid core
$MRTX 3.34% will keep my 2.02% paid core
$TPTX 3.34% will keep my 2.02% paid core
$SDGR sell all
$RVMD sell all
$ERAS sell all
$RLAY sell all
$RTPX sell all
Protein Degraders:

$ARVN sell all
$KYMR 1.35% will keep my .67% core
$CCCC 1.35% will keep my .67% core
$GLUE 2.02% will keep my .67% core
Read 8 tweets
22 Sep
#Molecular_Glue

A look at how molecular glue works.
1/ In previous Targeted Protein Degrader threads, I went over the basic process of how the E1 enzyme adds the phosphate group to the ubiquitin molecule. It then passes it to the E2 enzyme which binds to the E3 ligase as a complex.
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Read 6 tweets

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