1. An FCS insert scenario proposed by @daoyu15 explained by @pathogenetics thus:
"Some say the FCS is not the right sequence "Man" would design, but here @daoyu15 presents the exact workflow "Man" would use to generate the FCS sequence see in COVID-19, with a design rationale"
"Some say the FCS is not the right sequence "Man" would design, but here @daoyu15 presents the exact workflow "Man" would use to generate the FCS sequence see in COVID-19, with a design rationale"
https://twitter.com/Daoyu15/status/1455474290262884355
2. Adaptation in CaLu-3 cell lines
"adaptation in CaLu-3 actually reverses changes that happened in VERO E6. P681 and RRAR is fine-tuned to growth in CaLu-3 cell cultures"
"adaptation in CaLu-3 actually reverses changes that happened in VERO E6. P681 and RRAR is fine-tuned to growth in CaLu-3 cell cultures"
https://twitter.com/Daoyu15/status/1442131973883842575
3. Experiments with HCoV-OC43 Cleavage Sites
"HCoV-OC43 cleavage site RRSRR was introduced (in an experiment by Belouzard et al, 2009). A similar experiment in China could use either RRARR or RRSRR from MHV-JHM or HCoV-OC43 via a "Lazy Loop Insertion"
"HCoV-OC43 cleavage site RRSRR was introduced (in an experiment by Belouzard et al, 2009). A similar experiment in China could use either RRARR or RRSRR from MHV-JHM or HCoV-OC43 via a "Lazy Loop Insertion"
https://twitter.com/Daoyu15/status/1456041615479377924
4. RRSRR may change to RRARR (Glycosylation) during passage by a single TCA->GCA change, but it is most likely that they introduced the MHV-JHM cleavage site, if it was intended to be tested in mice.
https://twitter.com/Daoyu15/status/1456042240527069189
5. "one NL63 paper used the RRARR site"
"Note that RRXR is in the other engineered site for their NL63 construct. This means that a direct engineered insertion of RRAR is also possible, again between the R!S in QTQTNSRS, same as RRARR"
researchgate.net/figure/Enginee… (Shulla, 2011)
"Note that RRXR is in the other engineered site for their NL63 construct. This means that a direct engineered insertion of RRAR is also possible, again between the R!S in QTQTNSRS, same as RRARR"
researchgate.net/figure/Enginee… (Shulla, 2011)
unroll @daoyu15 FCS insert scenario @threadreaderapp
• • •
Missing some Tweet in this thread? You can try to
force a refresh
