1. Document Trail (EHA)

From the Intercept's FOIA release:

Understanding-Risk-Bat-Coronavirus-Emergence-Grant-Notice

documentcloud.org/documents/2105…
2. Page 526

"Viral isolates will remain at the Wuhan Institute of Virology initially. Isolates, reagents and any other
products, will be made available to other NIH-funded researchers via applicable WIV and EHA Material Transfer Agreements and/or icensing agreements"
3. Cells (Page 203)

• Records are maintained for each of the cell lines regarding
1. the origin of the cell lines
2. when they were resuscitated
3. number of passages
4. all test results
5. any unique distinguishing growth behavior
6. any known genetic features.
4. Cell lInes (Page 203)

When receiving cell lines, lab members initially keep them isolated from other authenticated cell lines until mycoplasma testing & STR marker profiling is performed

All cell lines must be authenticated before commencing experimental work with them
5. Page 528

Recombinant and Wildtype Viruses and Mutant Derivatives.

All viruses will be validated and certified pure of contaminating viruses prior to use or shipment to other laboratories
6. Audit Trail (Page 527)

"UNC & WIV will implement an "audit trail" that tracks animals used in experimental investigations (Aim 3) from parents, through birth, shipment, experimentation, results, QC & analyses, providing outside researchers the ability to track experiments.."
7. Specific Aim 3 is on Page 512 and 272

Humanized mice for experimental infection for Specific Aim 3 (Page 512)

Specific Aim 3: Testing predictions of CoV inter-species transmission (Page 272)
8. RPPR reporting requirements

Research conducted at the following site(s) must be reported in your RPPR:

lnstitut Pasteur du Cambodge, CAMBODIA
King Chulalongkorn Memorial Hospital, THAILAND
Hanoi Agricultural University, VIETNAM
National Animal Health Laboratory, LAOS
9. Also:
San Pya Clinic, BURMA
Primate Research Center at Bogor Agricultural University, INDONESIA
Conservation Medicine, Ltd, MALAYSIA
10. Hence (from @whitecoatwaste lawsuit FOIA)



"we would like to request prior approval for collecting non-human animal samples in 7 countries:
Cambodia, Indonesia, Lao PDR , Malaysia, Myanmar, Thailand, and Vietnam" (Page 100)

scribd.com/document/53702…
Back in 2014, Dr. Yang Zhun -Zhi, Head of infectious disease surveillance, Yunnan CDC, collected 121 Bat Fecal Samples for CoV surveillance.

From: Intercept FOIA

Understanding-Risk-Bat-Coronavirus-Emergence-Grant-Notice

documentcloud.org/documents/2105… (Page 166)
11. Tweets 1 to 9 in this thread reveal documents and records held by EHA, UNC, WIV and WHU that should be made available for public scrutiny.

“el que no llora no mama”

If you don't cry, you won't get milk!

unroll Lab audit trail @threadreaderapp

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More from @BillyBostickson

7 Nov
1. From @humblesci MERS contamination summary:

"While reverse genetics system used by WIV for the NIH funded EHA project utilized a MERS-CoV backbone & swapped in HKU4r-CoV components

WIV seems to have been simultaneously using a HKU4r-CoV backbone for independent research"
2. WIV Spike Gene Swaps?

"Potentially the WIV may have been attempting to swap in the MERS-CoV spike gene using targeted RNA recombination (Masters and Rottier, 2005) or perhaps experimenting with a pseudotyped MERS-CoV spike vector"
3. That is a significant claim

"the WIV appears to have been simultaneously using a HKU4r-CoV backbone for independent research"

So while doing EHA NIH funded work, at the same time they were "experimenting" with the reverse!
Read 7 tweets
6 Nov
1. Biosafety Experts agree:

Reporting all biosafety errors could improve labs worldwide and increase public trust in biological research

phys.org/news/2021-10-b… via @physorg_com
2. we believe a more transparent and comprehensive system of reporting biological incidents to a neutral third party would help reduce the number of laboratory incidents
3. it could be broadened to include all kinds of incident data. In our opinion, an international version of such a reporting system would also help reduce the number and severity of laboratory incidents, both locally and worldwide
Read 4 tweets
4 Nov
1. Another #DARPA character - Jean Paul Chretien

Biological Technologies Office (BTO) Program Manager

He joined DARPA as a program manager in 2020.

His interests include disease and injury prevention, operational medicine & biothreat countermeasures.

darpa.mil/staff/cdr-jean… ImageImageImage
2. LinkedIn Profile - Jean Paul Chretien

"JP Chretien is a Navy doctor & Program Manager at the DARPA, in the Biological Technologies Office. Previously, JP led the Pandemic Warning mission in the Department of Defense"

linkedin.com/in/jpchretien/
2. LinkedIn Profile Screenshots ImageImageImageImage
Read 16 tweets
4 Nov
1. Introducing Darkcides

The database contains information for geographical distribution, ecological status, species traits, and parasites and hyperparasites for 679 bat species known to occur in caves or use caves in their life histories.

darkcides.org
2. DarkCideS 1.0, a global database for bats in karsts and caves

The database contains 6746 georeferenced occurrences for 402 cave-dwelling bat species from 2002 cave sites in 46 countries and 12 terrestrial biomes.

authorea.com/doi/full/10.22…
3. Authors include @AliceCHughes
Read 5 tweets
3 Nov
Recent @WhiteCoatWaste FOIA release

Laos to WIV, 2018!

"LAOS PI or Co-Investigators to conduct field
trip to assess markets, identify wildlife in them & arrange for shipment of bats and other high-risk host species to WIV Laboratory in China"

scribd.com/document/53702…
All Roads & Bats lead to the Wuhan institute of Death
If PD won't tell us, then these people need to be asked some questions about exactly aht samples they sent to WIV.
Read 17 tweets

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