There is a new paper out discussion MSH3 homology to this sequence.

The tables in the supplement demonstrate the authors never blasted this sequence against 16S databases.

Had they done so, they probably wouldn’t have submitted that paper.
There is no E value to this sequence
Here is the MSH3 paper.
Note the supplement doesn’t look at 16S sequence. This is one of the most common sequences on Earth and this 19bp has a 10-11bp match to it. This destroys its E value.

It’s like finding water on Earth. Doesn’t 📌 you down.

frontiersin.org/articles/10.33…
Description of E values
These are the claims. The MSH3 paper points to SEQ ID 11652. This is not in the claims.
So Moderna doesn’t have any patent rights over this sequence.

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More from @Kevin_McKernan

Feb 18
Valentine’s Week is the perfect time to zero in on the Mating Type Loci in Psilocybe cubensis.

Psilocybe is believed to have 4 mating types (tetra polar) and they won’t grow near each other if they are not compatible. This is a pheromone affect. So where is this in the genome? Image
One hint revealed itself as we were mapping p.envy HiC reads to the new p.envy reference genome. While these genomes share a strain name, they are from spores from different providers. Spores should multiple mating types.

Why does Chromosome 1 have 10x more variation? Image
So we mapped more genomes and looked at SNP density across the chromosomes and Bingo! Image
Read 4 tweets
Feb 15
This school is a disgrace.
@MiddlesexSchool
Why don’t you recognize the Covid recovered?

How does it feel to be discriminatory towards people who recovered from C19 and have better immunity than your cultish obsession over a vaccine that doesn’t stop transmission?

Woke cult.
I once donated my time to present genomics to your students.

Never again will I help your discriminatory school.
Over 150 articles that demonstrate the Covid recovered have better immunity than your experimental liability free vaccine that doesn’t stop transmission.

How can a school be this dumb?

Or are you too woke to read new data?

google.com/amp/s/brownsto…
Read 5 tweets
Feb 14
The Persistence length of dsRNA is an interesting thought.
4.2kb is about 1um in length. 43T of these is 43,730 kilometers.
The Circumference of the Earth is ~40,000km.
A 100ug injection is enough degradation resistant RNA in your body to circle the Earth. Image
Caveat... The shot is 99+% ssRNA (in theory) which has a shorter persistence length since it folds on itself but for illustrative purposes, dsRNA is shown. The 4.2Kb is the length of the ssRNA in the shot.
Read 5 tweets
Feb 11
The Antartica metagenomic samples that @jbloom_lab nicely covered has some quirks.
NCBI claims its Illumina Data
Fastq files have headers that look like MGISeq data.

So I decided to take a look at their Adaptor sequences as each sequencer has their own unique flow cell primers
TrimGalore indeed confirms these are MGISEQ reads.
This paper has the MGISEQ adaptor sequences-
frontiersin.org/articles/10.33…
Why does this matter? The authors posit that this could be a result of the high index hopping problem seen on Illumina platforms.
MGISEQs documented index hopping rate is orders of magnitude lower than Illumina.
bmcgenomics.biomedcentral.com/articles/10.11…
Read 9 tweets
Feb 7
Psilocybe cubensis assembly in NCBI.
This couldn't be done without @PhaseGenomics or @PacBio

ncbi.nlm.nih.gov/Traces/wgs/JAF…
These HiC maps were performed on spores which express no controversial compounds.
Illumina libraries are also generated with spore preps.
Read 7 tweets
Jan 26
Carl Fuller et al are at it again.

This is wild. 16,384 CMOS chip capable of single molecule movies at each pixel.

Each pixel has a protein nanowire bridging it. These are assembled with electrophoresis.

Once wired, click chemistry binds one molecule.

pnas.org/content/pnas/1…
20nM gap for the nanowire peptide bridge.

1000Hz read outs. 0-400pAmp sensors.
Chip features
Read 5 tweets

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