Vax longevity in the blood is buried in one of my posts.
It deserves its own thread
The title of this paper goes out to 28 days. Full seq is in NCBI.
3 full vax Sequences deposited.
This one is 15 days post jab.
This one is 5 days post jab.
Pfizer claimed it was gone in 24 hours.
Keep in mind normal C19 primers don’t amplify the vax due to the heavy codon optimization they did.
So no one is really looking for it.
When people do look… no one is listening.
To get full length sequencing of the virus, CDC asks for samples to be less than a CT of 28. So for this group to find it in plasma, there has to be a lot of it.

Keep in mind 80T of these 1um in length molecules can circle Earth.
That’s about 13M molecules per UL if you have 6L of blood.
Your body is 87L is not much less if it distributes everywhere.
~1M copies/ul across your whole body.
NEB calculator has 100ug at 43T molecules of a ~4.2kb ssRNA molecule.
2 shots= 86T
Booster even worse.

nebiocalculator.neb.com/#!/ssrnaamt
Earth =~40,000 Km circumference
1million microns/meter
1billion microns/Km
1trillion microns/1,000 Km
40Trillion microns/circumference

Each Vax mRNA = 1um.
Persistence length of mRNA =~4200bp/micron.
This study isn’t published yet so we don’t know if they stopped measuring at 28 days? Likewise, certain tissues may have higher concentrations than others.

Other studies show longer duration in GC/Lymphnodes.
For those not used to working with microliters…
That spot on his cheek is a microliter.

The shot will give you ~1Million copies of the mRNA in every microliter of your body assuming a uniform distribution to your 87L body.
43Trillion mRNAs per 100ug shot.
87million microliters per 87L.
~500,000 copies/ul
2 shots = 1M/ul
The Pfizer docs have a longevity study.
They didn’t radiolabel the mRNA.
They radiolabelled cholesterol as a proxy for the LNP bio distribution.
That’s whack.

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More from @Kevin_McKernan

Feb 25
Rudi Jaenisch was harassed by the woke mob for publishing a paper that could incite "vaccine hesitancy".

Looks like his hesitancy was was in order.
This paper demonstrates the BNT162b2 mRNA is getting reverse transcribed into DNA. It DOES NOT show

google.com/url?sa=t&rct=j…
Integration into the genome... yet. They need long read Whole genome sequencing to prove that which Im sure is currently underway. Rudi showed it was integrated but was critiqued that it could be an artifact of making sequencing libraries. This paper fills in many questions. Image
The study was well controlled with RNAse confirmation that the target is in fact DNA and not RT of RNA.
Team "Scientific Censorship" has a lot of explaining to do. Many people on this team were vocal opponents of the CRISPR baby experiments run in China. Now they are guilty of Image
Read 19 tweets
Feb 21
There is a new paper out discussion MSH3 homology to this sequence.

The tables in the supplement demonstrate the authors never blasted this sequence against 16S databases.

Had they done so, they probably wouldn’t have submitted that paper.
There is no E value to this sequence
Here is the MSH3 paper.
Note the supplement doesn’t look at 16S sequence. This is one of the most common sequences on Earth and this 19bp has a 10-11bp match to it. This destroys its E value.

It’s like finding water on Earth. Doesn’t 📌 you down.

frontiersin.org/articles/10.33…
Description of E values
Read 5 tweets
Feb 18
Valentine’s Week is the perfect time to zero in on the Mating Type Loci in Psilocybe cubensis.

Psilocybe is believed to have 4 mating types (tetra polar) and they won’t grow near each other if they are not compatible. This is a pheromone affect. So where is this in the genome? Image
One hint revealed itself as we were mapping p.envy HiC reads to the new p.envy reference genome. While these genomes share a strain name, they are from spores from different providers. Spores should multiple mating types.

Why does Chromosome 1 have 10x more variation? Image
So we mapped more genomes and looked at SNP density across the chromosomes and Bingo! Image
Read 4 tweets
Feb 15
This school is a disgrace.
@MiddlesexSchool
Why don’t you recognize the Covid recovered?

How does it feel to be discriminatory towards people who recovered from C19 and have better immunity than your cultish obsession over a vaccine that doesn’t stop transmission?

Woke cult.
I once donated my time to present genomics to your students.

Never again will I help your discriminatory school.
Over 150 articles that demonstrate the Covid recovered have better immunity than your experimental liability free vaccine that doesn’t stop transmission.

How can a school be this dumb?

Or are you too woke to read new data?

google.com/amp/s/brownsto…
Read 5 tweets
Feb 14
The Persistence length of dsRNA is an interesting thought.
4.2kb is about 1um in length. 43T of these is 43,730 kilometers.
The Circumference of the Earth is ~40,000km.
A 100ug injection is enough degradation resistant RNA in your body to circle the Earth. Image
Caveat... The shot is 99+% ssRNA (in theory) which has a shorter persistence length since it folds on itself but for illustrative purposes, dsRNA is shown. The 4.2Kb is the length of the ssRNA in the shot.
Read 5 tweets
Feb 11
The Antartica metagenomic samples that @jbloom_lab nicely covered has some quirks.
NCBI claims its Illumina Data
Fastq files have headers that look like MGISeq data.

So I decided to take a look at their Adaptor sequences as each sequencer has their own unique flow cell primers
TrimGalore indeed confirms these are MGISEQ reads.
This paper has the MGISEQ adaptor sequences-
frontiersin.org/articles/10.33…
Why does this matter? The authors posit that this could be a result of the high index hopping problem seen on Illumina platforms.
MGISEQs documented index hopping rate is orders of magnitude lower than Illumina.
bmcgenomics.biomedcentral.com/articles/10.11…
Read 9 tweets

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