Hutchinson Lab Profile picture
Sep 15, 2022 9 tweets 8 min read Read on X
🚨New pre-print!🚨
biorxiv.org/content/10.110…
Viruses are very small things with very big effects. During the #SARSCoV2 pandemic, tiny changes in molecular biology had huge impacts on people’s lives. How could we communicate about this clearly?
🦠#scicomm #sciart🦠
(1/9)
In a new study with @CVRinfo @GSofASimVis and @COGUK_ME, @sarahiannucci1 explains how she used animations and interactive visualisations to help explain the threat of #SARSCoV2 variants of concern (#VOCs; 2/9)
There were, of course, plenty of data out there, collected by groups like @COGUK_ME. But these sites were (quite reasonably) aimed at expert audiences.

Could the details of new SARS-CoV-2 variants be explained in a way that was accessible for the public? (3/9) Screenshot of the COG-UK/ME...
To do this, @sarahiannucci1 created the #SARSCoV2 Spike Protein Mutation Explorer, an interactive app that explains variants of concern through animations… (4/9)
… interactive models (5/9)… Screenshot of the SSPME app...
… and explanations of how the molecular details of #SARSCoV2 #VOCs changed the way the virus impacted our lives (6/9) Screenshot of the SSPME sho...
Sarah built this app for her MSc with @UofGMVLS and @GSofASimVis, with support from #MattPoyade, @robertson_lab, @_wharvey, @blJOg and @COGUK_ME

She did a fantastic job on this and won the @GSofA’s 2021 Chairman’s Award 🏆 (7/9)
Check out the app yourself here:

sc2-application.itch.io/sars-cov-2-mut…

… and have a look at the paper for details on how visual #scicomm improved molecular biology communication (8/9)

Finally, if you’re interested in the technical details of how Sarah built her app: a book chapter describing this is in press 📔
(watch this space...👀)
(9/9) The login screen of the SAR...

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More from @CVRHutchinson

Jun 24
Some interesting reading on viruses in milk 🐄🦠
Recently we and others looked at the effects of pasteurisation on H5N1 in milk (very effective) and the risks of 'raw' milk (very risky).
But what about unpasteurised cheese ? The experiment sounded like microbiological chaos... A cheese board showing various soft cheeses
... and to be clear we haven't done it ourselves, partly because everyone in the lab looks horrified whenever I suggest it. But people have done it in the past for another bovine virus, foot and mouth disease: ...sciencedirect.com/science/articl…
... in this 1976 paper, John Blackwell reports experimentally infecting cows with FMDV and then setting out on a home cheese making project, eventually producing batches of virus-laden cheddar, camembert and mozarella...
Read 9 tweets
Jun 3
🚨New Pre-Print🚨
With #H5N1 #flu shed in cow’s milk in the USA, we worked with @cvrinfo, @roslininstitute, @Pirbright_Inst & @APHAgovuk to ask if pasteurisation would make it safe
🐮🦠🥛🌡️
TL;DR: pasteurisation’s effective, raw milk can be infectious
medrxiv.org/content/10.110…
There are lots of good reasons to pasteurise milk, which is done by heating at 63C for a long time or 72C quickly. But we hadn’t previously had to worry about influenza in milk
Would pasteurisation work here too? Fresian cows
We tested this in labs, where we could vary the times and temps carefully (you then extrapolate to actual pasteurisation equipment)
Using a panel of influenza viruses in milk we found that they were all effectively inactivated before reaching pasteurising times at either temp Two graphs showing multiple lines (for virus titre) dropping rapidly on heating at 63C and 72C
Read 11 tweets
Feb 10, 2023
In a new paper led by @annasimsbiol, we find that influenza infections divide your throat into tiny territories, and ask:
why don’t these viruses want to be friends?

Paper doi.org/10.1371/journa…
Commentary doi.org/10.1371/journa…

A 🧵 An image showing two infected regions, one green and one mag
Some background: If two viruses get into the same cell, they can genes exchange (basically they can breed). This is really important for viral evolution. A dramatic example is when different strains of influenza A virus (IAVs) use coinfection to generate pandemic strains A schematic showing a green and magenta virus coinfecting a
Many viruses actively push back against coinfection, changing an infected cell until it becomes resistant to infection by related viruses. This effect is known as ‘superinfection exclusion’ (SIE) A schematic showing a cell infected by a green virus which,
Read 23 tweets
Jun 9, 2022
In a new preprint led by @annasimsbiol, we ask:
Why Don’t These Viruses Want to be Friends?
A 🧵...
biorxiv.org/cgi/content/sh… An image showing two infected regions, one green and one mag
Coinfection is an important aspect of viral evolution. If two viruses can get into the same cell, they can undergo genetic exchange. A dramatic example of this is when different strains of influenza A virus (IAVs) use coinfection to generate novel pandemic strains A schematic showing a green and magenta virus coinfecting a
However, many viruses actively push back against coinfection. In a variety of ways, they change an infected cell until it becomes resistant to infection by related viruses. This effect is known as superinfection exclusion (SIE) A schematic showing a cell infected by a green virus which,
Read 17 tweets
Sep 21, 2021
A quick Tweetorial about our new paper with the #BoutellLab, which @CharmanMatthew led during his PhD @CVRinfo

Alternative title: ‘Why are virologists’ cells stupid and does this matter?’ 🧵 (1/N)
If you work with human influenza viruses in the lab you most likely grow them in MDCK cells, or possibly MDBK cells, or maybe A549 cells if you are fussy enough to want a cell line that comes from (a) the right organ system and (b) the right species of animal (2/N)
These cell lines are super-convenient – they grow forever, and it’s so much easier to grow influenza in them than in, say, human bronchial epithelial cells. Great, right? (3/N) Influenza does not like growing in primary lung cells
Read 11 tweets
Dec 31, 2020
Earlier this year, @Scient_Art collaborated with us to produce one of the first detailed 3D models of the #SARSCoV2 virus particle. To round off 2021 she's updated her model, and it looks great (1/N) Image
... the first model drew heavily on existing work on related viruses (SARS-CoV-1 and MHV). The updated model has an improved representation of the spike protein, building on the detailed model from @RommieAmaro's lab (pubs.acs.org/doi/10.1021/ac…) (2/N) Image
The spacing and flexing of the spikes was also updated based on cryoEM data, particularly from the labs of @BriggsGroup (disq.us/t/3r34r8p) and @drlisai (doi.org/10.1016/j.cell…) (3/N) Image
Read 7 tweets

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