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fortheloveofmicrobiology Profile picture
@fortheloveofmi2
Dec 26, 2022 • 5 tweets • 4 min read • Read on X
Candida albicans on chocolate agar, with starry projections

#Fortheloveofmicrobiology #clinicalmicrobiology #mmidsp #microrounds #IDpath #ASMClinMicro
#MicroTwitter #WomenInSTEM #WomeninMicrobiology #STEM #medtwitter #ClinMicro #microbiologypakistan #PathBugs Image
Yeast will grow on bacteriological media (sheep blood agar and chocolate agar).

They may appear as small, creamy or white colonies that are somewhat more raised than staphylococcal colonies.
A presumptive identification of C albicans can be made by observing pasty,yellow-white colonies from which colony projections, often referred to as "feet," extend out from the margins.
These feet have typically been considered a characteristic of C albicans
These are actually hyphal elements radiating from colonies on blood-containing media within 48 hours of initial incubation.

These colonies with “feet,” which can also resemble stars and can be seen with Candida dubliniensis.
According to ASM, colonies with feet may also be observed in up to 25% of C. tropicalis.

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More from @fortheloveofmi2

fortheloveofmicrobiology Profile picture
May 7, 2023
An eerie video of a moving Enterobius vermicularis worm, shared by Sabir Hanif Hanif

#Fortheloveofmicrobiology #clinicalmicrobiology
#mmidsp #microrounds #IDpath #ASMClinMicro
#MicroTwitter #WomeninMicrobiology #STEM #medtwitter #ClinMicro #microbiologypakistan #PathBugs twitter.com/i/web/status/1…
Enterobius vermicularis, also known as pinworm, is a common intestinal parasite that infects humans. These worms are small, about 1 cm, are white or translucent in color. They are easily visible to the naked eye and can sometimes be seen moving around the anal area.
The video shows a moving Enterobius vermicularis worm with its distinctive cylindrical shape and tapered end. The worm moves by contracting and relaxing its muscles in a wave-like motion, allowing it to crawl or wriggle forward.
Read 6 tweets
fortheloveofmicrobiology Profile picture
Jan 3, 2023
Wet mount and gram stain showing free hooklets of Echinococcus spp on cyst fluid from a 75 yr old F. Circled the hooklets in following pictures.

#Fortheloveofmicrobiology #clinicalmicrobiology #microrounds #IDpath #ASMClinMicro
#MicroTwitter #ClinMicro #microbiologypakistan
Echinococcosis or hydatid disease is caused by the larval stage of the dog tapeworm, Echinococcus granulosus. The definitive host for this disease is the dog or other canids and the intermediate hosts are cattle,sheep,pigs,goats or camels. Man is an accidental intermediate host
Hydatid disease in humans is potentially dangerous depending on the location of the cyst. Some cysts may remain undetected for many years until they become large enough to affect other organs. Symptoms are then of a space occupying lesion.
Read 6 tweets
fortheloveofmicrobiology Profile picture
Nov 20, 2022
India ink preparation of CSF from a patient with cryptococcal meningitis showing the budding yeast cells of C. neoformans surrounded by a characteristic wide gelatinous capsule. The yeasts also show narrow-base budding and characteristic variation in size.
The space occupied by the capsule shows as a clear space between the gray background of the ink particles and the refractile edge of the cell.
India ink/nigrosin stain is a negative, acid stain. This means that the dye easily gives up a hydrogen ion (proton) and the chromophore of the dye becomes negatively charged. Since the surface of most organims cells is negatively charged, the cell surface repels the dye.
Read 9 tweets
fortheloveofmicrobiology Profile picture
Nov 18, 2022
CSF received from a 12 year old child with Ewing sarcoma

India ink preparation showing rounded yeast cells surrounded by a halo, highly suggestive of Cryptococcus neoformans
#Fortheloveofmicrobiology #clinicalmicrobiology #mmidsp #microrounds #ASMClinMicro #microbiologypakistan Image
India ink stain, previously known as the Nigrosin stain, is a quick, low-resource method. It is widely used for the microscopic detection of cryptococci in CSF. Its a negative stain used to determine the organism’s cellular morphology.
In the India ink preparation, stain fills the background and the thick polysaccharide capsule does not take up the stain, resulting in an appearance of a refractile large white circle against a dark background, giving the appearance of a halo of light
Read 8 tweets
fortheloveofmicrobiology Profile picture
Feb 20, 2022
Many times when susceptibility testing is done for P. aeroginosa, a scenario similar to shown in the picture is encountered.
We see that TZP has produced a D shape on the IPM side. This might look very similar to the phenomenon seen in gram positive ICR strains. So what is it?
Published data says that this result is most likely due to
inducible expression of the P. aeruginosa AmpC beta-lactamase.
Certain enteric
(Serratia, Providencia, Citrobacter, Enterobacter, Morganella) and non-enteric organisms (P. aeruginosa, Aeromonas) can up-regulate expression of
their chromosomally-encoded ampC genes in response to sub-inhbitory concentrations of certain
b-lactam antibiotics
Read 10 tweets
fortheloveofmicrobiology Profile picture
Jul 8, 2021
E.coli, demonstrating the Eagle effect

The Eagle effect, Eagle phenomenon, or paradoxical zone phenomenon, named after Harry Eagle, originally referred to the paradoxically reduced antibacterial effect of penicillin at high doses.
Though recent usage generally refers to the relative lack of efficacy of beta lactam antibacterial drugs on infections having large numbers of bacteria. The former effect is paradoxical because the effectiveness of an antibiotic generally rises with increasing drug concentration.
In other words, the Eagle effect describes a phenomenon where decreased bactericidal activity of an organism is taking place despite of exposure to higher levels of an antibiotic.
Read 9 tweets

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