They may appear as small, creamy or white colonies that are somewhat more raised than staphylococcal colonies.
Nov 20, 2022 • 9 tweets • 4 min read
India ink preparation of CSF from a patient with cryptococcal meningitis showing the budding yeast cells of C. neoformans surrounded by a characteristic wide gelatinous capsule. The yeasts also show narrow-base budding and characteristic variation in size.
The space occupied by the capsule shows as a clear space between the gray background of the ink particles and the refractile edge of the cell.
Nov 18, 2022 • 8 tweets • 3 min read
CSF received from a 12 year old child with Ewing sarcoma
India ink preparation showing rounded yeast cells surrounded by a halo, highly suggestive of Cryptococcus neoformans #Fortheloveofmicrobiology#clinicalmicrobiology#mmidsp#microrounds#ASMClinMicro#microbiologypakistan
India ink stain, previously known as the Nigrosin stain, is a quick, low-resource method. It is widely used for the microscopic detection of cryptococci in CSF. Its a negative stain used to determine the organism’s cellular morphology.
Feb 20, 2022 • 10 tweets • 5 min read
Many times when susceptibility testing is done for P. aeroginosa, a scenario similar to shown in the picture is encountered.
We see that TZP has produced a D shape on the IPM side. This might look very similar to the phenomenon seen in gram positive ICR strains. So what is it?
Published data says that this result is most likely due to
inducible expression of the P. aeruginosa AmpC beta-lactamase.
Jul 8, 2021 • 9 tweets • 1 min read
E.coli, demonstrating the Eagle effect
The Eagle effect, Eagle phenomenon, or paradoxical zone phenomenon, named after Harry Eagle, originally referred to the paradoxically reduced antibacterial effect of penicillin at high doses.
Though recent usage generally refers to the relative lack of efficacy of beta lactam antibacterial drugs on infections having large numbers of bacteria. The former effect is paradoxical because the effectiveness of an antibiotic generally rises with increasing drug concentration.