๐•ต๐–”๐–๐–“ ๐•ต๐–†๐–ˆ๐–”๐–‡๐–˜ ๐Ÿ‡ฌ๐Ÿ‡ง Profile picture
Christian, medical data science, immunology. ๐’ซโ„ฏ๐“‡๐“ˆโ„ด๐“ƒ๐’ถ๐“ โ„ด๐“…๐’พ๐“ƒ๐’พโ„ด๐“ƒ๐“ˆ โ„ด๐“ƒ๐“๐“Ž. --- ๐Ÿ‡ณ๐Ÿ‡ฑ @Chr1stenJohn JohnJL@bsky.social ๐—–๐Ÿ†โ“”๐Ÿ…๐šโ“ค๐ซ๐Ÿ…”๐“ˆ ๐• ๐š โ“›๐Ÿ…พ๐˜ทโ“”!
๐•ต๐–”๐–๐–“ ๐•ต๐–†๐–ˆ๐–”๐–‡๐–˜ ๐Ÿ‡ฌ๐Ÿ‡ง Profile picture 2 subscribed
Sep 10 โ€ข 8 tweets โ€ข 2 min read
Remarkable research conclusions.
SARS-2 infection is directly associated with changes in the brain (due to brain infection).
The authors conclude that lockdown is the cause, without looking at infection as a putative cause.
The main argument of ... 1/3
nature.com/articles/s4158โ€ฆ

Image ...the authors is that they do not know about sex disparity of the virus.
Literature, however, does: more males died due to Covid, more females has long covid.
So data might be correct, unusually accelerated brain maturation in adolescents and that this accelerated ...
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May 13 โ€ข 11 tweets โ€ข 5 min read
New lethal late-onset disease after COVID-19.
Anti-MDA5+ dermatomyositis is characterised by rapidly progressive interstitial lung disease (ILD) &high mortality.
MDA5 is an RNA sensor & and a key pattern recognition receptor for the SARS-CoV-2 virus.

1/9 thelancet.com/action/showPdfโ€ฆ
Image Autoimmune disease triggered by SARS-CoV-2 infection noticed in 60 patients. Rapid & often lethal progression of autoimmune diseases, mechanistically and related to SARS-CoV-2 and found in COVID-19 aftermath.
2/9 Image
Apr 12 โ€ข 9 tweets โ€ข 5 min read
original antigenic sin #OAS deals with antibodies and might be important for protection from infection and herd immunity.

T-cell immunity might be key to protect from (serious) disease by eliminating infected cells and yielding cross-protective immunity. 1. Vaccine for T-cells protects from moderate-severe disease.
2. CD4, CD8 epitopes crucial for cross-protection against sever disease.
3. CD8 in protective immunity. pubmed.ncbi.nlm.nih.gov/38318376/
pubmed.ncbi.nlm.nih.gov/38318166/
pubmed.ncbi.nlm.nih.gov/38379003/
Apr 11 โ€ข 10 tweets โ€ข 4 min read
Does vaccination prevent immune responses against mutated SARS-CoV-2. #COVID #AntigenicSin
Paper seems to conclude this, but how sound are its conclusions on original antigenic sin?

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๐Ÿงตon virus immunology.nature.com/articles/s4146โ€ฆ Note that immunity is compared between
Unvaccinated and omicron (O) infection (inf).
3x Wildtype (WT) vaccine + O inf year later.
3x Wildtype (WT) vaccine, tested immediately.
SARS-CoV-2 immunity wanes in time
2/7 Image
Mar 9 โ€ข 9 tweets โ€ข 4 min read
COVID-19 is an important contributor to cardiovascular disease.
Therefore the @escardio has made a collection of information on this subject.


1/8escardio.org/Education/ESC-โ€ฆ COVID-19 affects many organ systems, including cardiovascular (CV) system.
COVID-19 remains a pervasive risk, not only for individuals with pre-existing CV diseases but also for those previously healthy, who may develop CV conditions as a consequence of SARS-2.

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Jan 31 โ€ข 10 tweets โ€ข 4 min read
Links between @UNRWA and #Hamas.

Sincee 07/10/2023
3000 UNRWA teachers celebrating 7/10.


Booby-trapped tunnel into a clinic run by UNRWA


Hamas Terror Tunnel Next to UNRWA School


1/7unwatch.org/report-unrwas-โ€ฆ
thetower.org/0808-dark-sideโ€ฆ
fdd.org/analysis/2023/โ€ฆ @WSJย โ€œOur intelligence indicates that out of approximately 12,000 UNRWA employees in the Gaza Strip, about 10% are Hamas or Islamic Jihad operatives, and another 50% are first-degree relatives of a Hamas operative,โ€

2/7jns.org/israel-calls-tโ€ฆ
Dec 28, 2023 โ€ข 10 tweets โ€ข 2 min read
Rechtmatigheid van oorlog wordt niet bepaald door aantallen slachtoffers. In WO2 vielen veel meer Duitse burgerslachtoffers te betreuren dan in de geallieerde landen. Waar de Nazi's nog respect hadden voor eigen of als eigen beschouwde burgers,
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offert Hamas eigen burgers als dekmantel voor hun terreuracties. Dat is strijdig met de mensenrechten. Hamas ziet echter financiรซle en politieke support als ze veel burgerslachtoffers kunnen claimen.
Hamas legt de schuld van alle slachtoffers bij Israรซl, zoals bij 2/8
Jan 23, 2023 โ€ข 10 tweets โ€ข 3 min read
Some interesting new molecular biology studies on SARS-CoV-2 that yield insight in the mechanisms of disease.

1/8
SARS-CoV-2 genes have functions in virus reproduction but also in:
- Undermining innate immunity by neutrophils
- Cell metabolism (glucose, hypoxia)
- Cell biology (mRNA splicing, transcription Protein polymerization, ER, Golgi)
nature.com/articles/s4158โ€ฆ
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Jan 21, 2023 โ€ข 16 tweets โ€ข 9 min read
๐Ÿ‡จ๐Ÿ‡ณ Chinese paper suggest that >4๐Ÿ’‰would be detrimental. It is cheered by antivaxxers.

Good for in-depth look at paper.
๐Ÿ’‰A. Paper
๐Ÿ’‰B. Publicity
๐Ÿ’‰C. Data shown
๐Ÿ’‰D. Data not shown
๐Ÿ’‰E. Method used
๐Ÿ’‰F. Human data
๐Ÿ’‰G. Discussion.
๐Ÿ’‰H. Conclusion.
๐Ÿ’‰I. Notes.

1/16 ๐Ÿ’‰A. Paper

๐Ÿ‡จ๐Ÿ‡ณ Chinese paper has provocative title:

"Extended SARS-CoV-2 RBD booster vaccination induces humoral and cellular immune tolerance in mice"

Spoiler:
The title promises much more than the article does.

sciencedirect.com/science/articlโ€ฆ

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Jan 21, 2023 โ€ข 8 tweets โ€ข 4 min read
Long COVID review.
>= 10% severe acute COVID19
>= 200 symptoms - multiple organ systems
>= 65 million individuals

After the acute disease by SARS-CoV-2, it is not over for a large subgroup.
nature.com/articles/s4157โ€ฆ

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Systemic disease:
Impact on heart, lungs, immune system, pancreas, gastrointestinal tract, neurological system, kidneys, spleen & liver, blood vessels & reproductive organs.

Long covid morbidity goes beyond direct mortality
hartblik.weebly.com/diseaseoutnumbโ€ฆ
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Jan 19, 2023 โ€ข 7 tweets โ€ข 3 min read
Paper investigates link between mRNA vaccination and myocarditis death. There were some worries about myocarditis in young men.

Evaluation of the evidence of 35 rare unexpected deaths after vaccination ๐Ÿ‡ฉ๐Ÿ‡ช.

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link.springer.com/article/10.100โ€ฆ Image 35 unexpected deaths examined.

- 10 were under 40 years. None of these died due to mRNA vaccination (1 had AstraZeneca vaccine).

- The authors link 5 cases (46-75 years) likely/possibly to mRNA vaccination.

2/6 Image
Jan 2, 2023 โ€ข 9 tweets โ€ข 4 min read
Nasal spray prevents 78% of SARS-2 infections.

Data sounds very interesting but some remarks.
- Young healthy participants ~ 34 (20s, 30s 40s)
- Many adverse events
- Juggling with numbers
- Many drop outs.
- Control data missing.
- Errors
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medrxiv.org/content/10.110โ€ฆ Data errors (3x the same in paper!):
๐Ÿงฎ135.544 = 27103 + 110441

It is not a major error but indicative for the data presentation.

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Jan 1, 2023 โ€ข 4 tweets โ€ข 3 min read
Persistent infections in gastric and gallbladder tissue after SARS-CoV-2 infection.
- Detection of nucleoprotein (infection only - not in vaccine!)
- Up to 1 year (9-12 months) after infection.
- Especially with young people.
- Observed in 1/3 patients
link.springer.com/content/pdf/10โ€ฆ
1/3 SARS-2 viral persistence has been described before in the gut. ncbi.nlm.nih.gov/pmc/articles/Pโ€ฆ
molecularcasestudies.cshlp.org/content/7/2/a0โ€ฆ
medrxiv.org/content/medrxiโ€ฆ

And found to be functionally important for the immune system 6 months after infection. 2/3
pubmed.ncbi.nlm.nih.gov/33461210/
Dec 25, 2022 โ€ข 9 tweets โ€ข 2 min read
๐Ÿ’‰Why are mRNA vaccines so effective?

After decades of trying to make mRNA stable enough to elicit immune responses they seem to outcompete all other vaccines:

๐Ÿ’‰>90% protection against severe disease compared to ~60% for other vaccine or previous infection

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๐Ÿ’‰Even better protection from severe disease after boosting.


What is different about mRNA vaccines that makes them so effective.
A. Virology
B. Immunology

2/8
Dec 23, 2022 โ€ข 16 tweets โ€ข 7 min read
Time for a deep dive into SARS-CoV-2 immunology after vaccination and infection. Iโ€™ll try to explain it also for non immunologists.
New observation: Booster mRNA vaccines induce antibody switch to IgG4, what does this mean?
science.org/doi/epdf/10.11โ€ฆ
pubmed.ncbi.nlm.nih.gov/36548397/
1/ It is known that immune responses could either protect against disease or exacerbate disease.
Early non-neutralizing, afucosylated IgG1 antibody responses are associated with COVID-19 severity.
pubmed.ncbi.nlm.nih.gov/35040666/
pubmed.ncbi.nlm.nih.gov/36385627/
2/
Nov 16, 2022 โ€ข 9 tweets โ€ข 3 min read
Covid reinfection has higher higher ratio (HR) of death (2.17) hospitalization (3.32) and sequelae #longcovid incl. pulmonary, cardiovascular, hematological, diabetes, gastrointestinal, kidney, mental health, musculoskeletal and neurological disorders.
1/
nature.com/articles/s4159โ€ฆ Hazard ratio in the left column. Note all are above he dotted line at 1 (same risk as 1. infection).
2/
Mar 2, 2022 โ€ข 4 tweets โ€ข 2 min read
Study showing intracellular LINE-1 reverse transcriptase in a liver cell line is able to perform RT activity on mRNA of Spike protein and allow DNA integration.
It is quoted as evidence that vaccination #Pfizer is dangerous, but
mdpi.com/1467-3045/44/3โ€ฆ
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1. The human liver cell line is a cancer cell line that could only survive in petri dish, not in a human.
2. LINE-1 can indeed perform RTase activity for mRNA but would not discriminate between virus and vaccine.
Involved in multiple human diseases. pubmed.ncbi.nlm.nih.gov/9621510/
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Mar 2, 2022 โ€ข 4 tweets โ€ข 2 min read
Remarkable paper.
Not peer reviewed but highly opiniated.
Ignoring completely the risk of a SARS-2 infection and not citing all the papers that give contra evidence.
Hard to call this science anymore. Heart-disease risk soars after COVID โ€” even with a mild case. nature.com/articles/d4158โ€ฆ
Long-COVID symptoms less likely in vaccinated people, Israeli data say.
nature.com/articles/d4158โ€ฆ
Feb 28, 2022 โ€ข 4 tweets โ€ข 2 min read
๐Ÿ‡บ๐Ÿ‡ฆ๐Ÿ‡ท๐Ÿ‡บ
My first thought was that #Ukraine would not stand a chance against #Russia in the lowlands, but #Putin dismantled the Russian army from information, GPS, gas and food.
The invading army is unprepared, similar to operation #Barbarossa.
Lessons from other wars show that understanding how the army is welcomed, local information, supply chains of gas and food are crucial for the military success. In general these are hard to manage in the mountains, but there are already challenges in the lowlands.
Aug 5, 2021 โ€ข 5 tweets โ€ข 4 min read
Testen, vooral in de context van bron- en contact onderzoek #BCO is de standaard en efficiรซntste manier virus epidemie bestrijding.

Vaccinatie gaat virus verspreiding niet voorkomen, en test, traceer en isoleer #TTI kan een lockdown voorkomen.
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hartblik.weebly.com/TTI.html ๐ŸŒง๏ธAantal #COVID19 besmettingen gaat in de herfst en winter toenemen.

๐Ÿ’‰Vaccinatie vermindert het aantal mensen dat ernstig ziek wordt met ~90%, maar als het aantal besmettingen 10x zo hoog wordt (2020 - 5%๐Ÿ‡ณ๐Ÿ‡ฑ) dan liggen de ziekenhuizen overvol.
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hartblik.weebly.com/vaccinatie.html
Apr 11, 2021 โ€ข 6 tweets โ€ข 3 min read
Interesting research and hypotheses on Adenovirus vector vaccines causing thrombosis. Might also be relevant for safety of Janssen J&J & Sputnik vaccine.
sciencemag.org/news/2021/04/hโ€ฆ Anti-Platelet Factor 4 (PF4) antibody seems not involved in #VITT - previously named #VIPIT.
Antibody-mediated vaccine-induced thrombotic thrombocytopenia.
assets.researchsquare.com/files/rs-40476โ€ฆ