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Christian Mosimann Profile picture
@chrmosimann
Apr 22, 2020 • 12 tweets • 7 min read • Read on X
Kicking off concurrent sessions #TAGC20, "Genetic Control of Development and Regeneration (#Zebrafish)!
@zoom_us tested for all speakers, @snillum1 & I will guide Q&A, 400+ attendees logged in, and go!
First talk by Daniel Zuppo (Tsang lab, UPittsburg) on foxm1 in proliferating cardiomyocytes upon heart injury.

Trx factors & beautiful stainings, what's not to ❤️!
#ZFish20
Next up, Wei Wang @Planaria1 @ScienceStowers on enhancers, their conservation (or lack thereof), and the variations in regenerative capacities in vertebrates.
Killifish transgenics tested in regeneration assays!
#ZFish20 #Killifish20?
Patti Murphy from the Granato lab at UPenn on Robo2 and Type-IV Collagen in axon regeneration is next...

...and that's how you share your beautiful PhD thesis work with 506 (and counting!) attendees in the current #ZFish20 session #TAGC20!
(my apologies if I miss any @ tagging in tweets - chairing, commenting, listening, tweeting is a bit much even from home!)
Moving to a different...scale:
Skin appendage formation decoded at the single-cell level using #zebrafish, from Lauren Saunders of the Trapnel lab in collab with @parichylab!
Handing over chairing to @snillum1, introducing Saulius Sumanas from Cincinnati Children's and his talk on what Etv2-deficient cells are up to when they can't become blood vessels.
#ZFish20 #lateralplatemesodermrocks
Brant Weinstein up now #Zfish20 #TAGC20 on how microRNAs control lymphangiogenesis - the "other" tubes!
574 attendees now, great to see the...shoal? is growing!
#ZFish20
Next up, Haoyu Wan from Ashley Bruce's lab at UToronto on actin and microtubule interactions in the #zebrafish yolk - the strings coordinating gastrulation!
#ZFish20
...and capping off a wonderful and technically nearly flawless (!) #ZFish20 #TAGC20 session: Kryn Stankunas from University of Oregon on the #zebrafish longfin mutant!
And done! First virtual #zebrafish session at #TAGC20 is a wrap.

Congrats and thanks to all speakers for sharing their research with all of us in our homes across the globe.

Looking fwd to the next sessions!
#ZFish20

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More from @chrmosimann

Christian Mosimann Profile picture
Jan 13, 2022
Remarks on faculty job searches (as 'tis the season):
Chances are, as a faculty candidate you apply to several job ads.

Dozens. More.

You wait. You hope.

(quick thread)
1/n
 #PILife #PDLife #AcademicJobs
Some places will respond with a rejection pretty quickly. Form letter.

Sometimes hilariously addressed to Dear [Applicant Name3].

Are you just a number? At this stage, likely.

Others never get back to you. Or years (!) later.

2/n
This is normal and as in any higher-level job with lots of competition.

The waiting, the rejections, and the no-responses are depressing. Discouraging. At times unhealthy.

"Does nobody care about my ideas? My science? Me?"

3/n
Read 16 tweets
Christian Mosimann Profile picture
Sep 22, 2020
As undergrad, I struggled immensely with this: reading and omg understanding papers.

Especially as non-native English speaker.

I did develop a system that helped me back then.

Bear with me it is pretty...mundane. 1/n

#reading #PhDLife #PDlife #undergradlife #papers #science
I printed each paper. And while reading I used a yellow, a green, and a pink highlighter.

When reading, I labeled every first mention of an abbreviation in green.

This helped me to find their meaning quickly again when deep into the text.

2/n
In pink, I labeled key questions and take-home messages.

If done correctly, you can look at pink sentences alone and recap the whole paper.

Peo-rip: first and last sentences of paragraphs often are pink sentences.

Pro-tip 2: write papers that have pink sentences.

3/n
Read 18 tweets
Christian Mosimann Profile picture
Aug 19, 2020
This is every molecular biologist's nightmare - vector and PCR product contamination across samples.

We can sequence so deep now that all kinds of details come to light!
I bet lots of us "reproduced" aspects of this in our own labs - i.e. a genotyping you do often starts to show up in every PCR you do, etc.

DNA always finds a way.

#mobio #devbio #benchwork
To add two anecdotes.

A good friend during my PhD did mass spec on Drosophila samples, and couldn't figure out what the predominant recovered peptides were. They overpowered everything else.

Human Keratin.

He made sure to wear gloves after this. 1/2
Read 4 tweets
Christian Mosimann Profile picture
Jul 7, 2020
Honored & grateful to receive the @ZDMSociety award in these trying times.

The award also recognizes our amazing international trainees using #zebrafish #devbio as cardiovascular disease model, first @UZH_en & now @CUAnschutz - and our fantastic collaborators!

Thx all🍾🐟!
Special shout-out to @FelkerAnastasia @karin_prummel @aburger2009, our collaborators @ClaudioCantu81 @d_zimmerli @KevinMMA13 @DanielaPanakova @PanakovaLab @George_Hausmann and all who were part of our BCL9 work cited in the award:
genesdev.cshlp.org/content/32/21-… Image
...and thx to all our current lab members @CUAnschutz, many on visas, not all on Twitter - onwards always:
@hannah_moran @AKocere @aburger2009 Image
Read 4 tweets
Christian Mosimann Profile picture
Jun 19, 2020
Happy our review on the lateral plate mesoderm is out @Dev_journal, with @karin_prummel & @Susan81643382 (open access).

Our first attempt at reviewing the fascinating, wacky, complex biology as Development at a Glance with poster!
#devbio #evodevo
dev.biologists.org/content/147/12… Image
The LPM forms several central cell fates and organ systems, including the heart, endothelium, blood, limb connective tissue, and much more.

This seemingly weird combo of downstream fates seems unrelated the adult body - but they all share a common LPM origin!
Our review now attempts to summarize how LPM's development (and possible evolution) ties these diverse downstream fates together.

We also discuss that not all "mesoderm" is equal:
the LPM forms as dedicated mesendoderm lineage early on, both in development and evolution.🧬🧬🧬
Read 8 tweets
Christian Mosimann Profile picture
Jun 19, 2020
Fun fact:
most PIs themselves never had (or sought) any actual formal training for any of this. Similar for teaching or mentoring.

Frequent attitude:
"winging it" is normal/badge of honor/funny.

Don't want to say it shows. But it totally does.
Few quick additions:

"Budgeting" or lab budgets are often not that complex. Salaries are pre-defined. Reagents over time sum up to an average consumption and can be estimated. And often you get help.

I always dreaded the book keeping but it's useful and gives peace of mind.
"People management" is a deep rabbit hole that many see as "learning by doing". Some is, indeed.

But workplace psychology, supervision, progress tracking et al. is done in industry & military since decades/centuries. This has language and framework. Useful to seek out.
Read 6 tweets

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