Christian Mosimann Profile picture
Sep 22, 2020 18 tweets 6 min read Read on X
As undergrad, I struggled immensely with this: reading and omg understanding papers.

Especially as non-native English speaker.

I did develop a system that helped me back then.

Bear with me it is pretty...mundane. 1/n

#reading #PhDLife #PDlife #undergradlife #papers #science
I printed each paper. And while reading I used a yellow, a green, and a pink highlighter.

When reading, I labeled every first mention of an abbreviation in green.

This helped me to find their meaning quickly again when deep into the text.

2/n
In pink, I labeled key questions and take-home messages.

If done correctly, you can look at pink sentences alone and recap the whole paper.

Peo-rip: first and last sentences of paragraphs often are pink sentences.

Pro-tip 2: write papers that have pink sentences.

3/n
In yellow, highlight text that you deem important to the story.

Caveat: at first you might as well pour yellow paint over the paper as you feel EVERYTHING is important.

(spoiler alert: it is not)

4/n
Structuring a paper this way facilitates understanding (find abbreviations,, key concepts), helps conceptualizing (what is important?), and shows how to get from A to B.

Add another color for techniques, external data, key references, et al.

5/n
Having such, uh, colorful papers (we printed papers as no tablets or laptops then) helped me tremendously when discussing papers in class and with fellow students.

@nmatasci will recall many a paper discussion with Walter Schaffner or Markus Noll....

6/n
More importantly though, this selective highlighting helped me to concentrate on what is what in a paper.

And it developed a (mundane) habit for me.

But wait a sec, you ask, what about figures....buckle up, folks.

7/n
Having terrible ideal printers (and for sure not color), figures became secondary when reading papers.

Yes, I love imaging and biology is beautiful - but hear me out. You can show me whatever you want in a figure. If the text doesn't hold up, you don't convince me.

8/n
Lots of folks put major emphasis on figures. Heck I do with our papers as they provide the evidence for the narrative!

But again, how can I tell you didn't cherry-pick your nicest staining/embryo/blot? How can you tell WE didn't?

Show me the context. In the text.

9/n
This leads to my original response to the tweet and @rita_strack's answer:

a well-written paper can be condensed to its first and last sentences in the results paragraphs (the "pink" sentences).

Not all the time for all paragraphs, but try it. Observe. Write this way.

10/n
Example:

Frist sentence explains what the experiments aims to show/hypothesis/idea. Fluff fluff fluff how we did stuff more fluff and fluff and fluff fluff fluff. Taken together, our observation suggests/supports the hypothesis/establishes X.

Cool, no? Rinse. Repeat.

11/n
I always love good discussions. A good discussion in hourglass style (starting broad, narrowing on the addressed problem, widening again) in itself can be the whole paper. And more literate, fluid than results.

Context. Significance. Impact. All in a good discussion.

12/n
Intros have been vilified as "cheap reviews" or "unnecessarily long".

But: many of a paper's readers might have never heard of a topic before. A good intro provides an awesome way to get into a field and a paper.

Intros have been my micro-reviews to learn lots of #devbio.

13/n
Still can't get over my take on figures? Maybe this helps:

Read the text first.

After you read it all, go only through the figures and trace back what the text told you.

Pro-tip: good figure titles strung together are a micro version of the paper (pink sentences!).

14/n
These steps and details helped me at least to make sense of papers when I started getting into science.

Over time, reading skill and speed refines, and you get better at capturing relevant info.

You train this. By doing. Like a sport.

15/n
I still recognize remnants of my initial reading system in how I read papers now.

Cool figures? Fine, but show me the narrative. The pink sentences that make sense.

And you can never ever stop reading. It is hard. Don't feel stupid. It is a struggle.

So is writing.

16/n
I could ramble on, but stop here. Read papers. Follow the references.

Find a way that suits YOU.

And remember. This is hard. And you will get better by continously working on paper reading.

Mundane but effective.

17/n
...ok one last thing:

Get your uni's VPN so you can access journal subscriptions/your library from home or from your phone.

Then you have no excuse not to read a particular paper right now ;).

You got this!
#keepreading

/fin

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More from @chrmosimann

Jan 13, 2022
Remarks on faculty job searches (as 'tis the season):
Chances are, as a faculty candidate you apply to several job ads.

Dozens. More.

You wait. You hope.

(quick thread)
1/n
#PILife #PDLife #AcademicJobs
Some places will respond with a rejection pretty quickly. Form letter.

Sometimes hilariously addressed to Dear [Applicant Name3].

Are you just a number? At this stage, likely.

Others never get back to you. Or years (!) later.

2/n
This is normal and as in any higher-level job with lots of competition.

The waiting, the rejections, and the no-responses are depressing. Discouraging. At times unhealthy.

"Does nobody care about my ideas? My science? Me?"

3/n
Read 16 tweets
Aug 19, 2020
This is every molecular biologist's nightmare - vector and PCR product contamination across samples.

We can sequence so deep now that all kinds of details come to light!
I bet lots of us "reproduced" aspects of this in our own labs - i.e. a genotyping you do often starts to show up in every PCR you do, etc.

DNA always finds a way.

#mobio #devbio #benchwork
To add two anecdotes.

A good friend during my PhD did mass spec on Drosophila samples, and couldn't figure out what the predominant recovered peptides were. They overpowered everything else.

Human Keratin.

He made sure to wear gloves after this. 1/2
Read 4 tweets
Jul 7, 2020
Honored & grateful to receive the @ZDMSociety award in these trying times.

The award also recognizes our amazing international trainees using #zebrafish #devbio as cardiovascular disease model, first @UZH_en & now @CUAnschutz - and our fantastic collaborators!

Thx all🍾🐟!
Special shout-out to @FelkerAnastasia @karin_prummel @aburger2009, our collaborators @ClaudioCantu81 @d_zimmerli @KevinMMA13 @DanielaPanakova @PanakovaLab @George_Hausmann and all who were part of our BCL9 work cited in the award:
genesdev.cshlp.org/content/32/21-… Image
...and thx to all our current lab members @CUAnschutz, many on visas, not all on Twitter - onwards always:
@hannah_moran @AKocere @aburger2009 Image
Read 4 tweets
Jun 19, 2020
Happy our review on the lateral plate mesoderm is out @Dev_journal, with @karin_prummel & @Susan81643382 (open access).

Our first attempt at reviewing the fascinating, wacky, complex biology as Development at a Glance with poster!
#devbio #evodevo
dev.biologists.org/content/147/12… Image
The LPM forms several central cell fates and organ systems, including the heart, endothelium, blood, limb connective tissue, and much more.

This seemingly weird combo of downstream fates seems unrelated the adult body - but they all share a common LPM origin!
Our review now attempts to summarize how LPM's development (and possible evolution) ties these diverse downstream fates together.

We also discuss that not all "mesoderm" is equal:
the LPM forms as dedicated mesendoderm lineage early on, both in development and evolution.🧬🧬🧬
Read 8 tweets
Jun 19, 2020
Fun fact:
most PIs themselves never had (or sought) any actual formal training for any of this. Similar for teaching or mentoring.

Frequent attitude:
"winging it" is normal/badge of honor/funny.

Don't want to say it shows. But it totally does.
Few quick additions:

"Budgeting" or lab budgets are often not that complex. Salaries are pre-defined. Reagents over time sum up to an average consumption and can be estimated. And often you get help.

I always dreaded the book keeping but it's useful and gives peace of mind.
"People management" is a deep rabbit hole that many see as "learning by doing". Some is, indeed.

But workplace psychology, supervision, progress tracking et al. is done in industry & military since decades/centuries. This has language and framework. Useful to seek out.
Read 6 tweets
Apr 22, 2020
Just co-chaired the first #ZFish20 concurrent session at #TAGC20 @GeneticsGSA.

Short thread after the fact:

1) WE CAN DO THIS!

1/7
2) Great technical support with setting up, etc. from the assigned technicians.

No need to sweat, and it was great to see enthusiastic smiles and humor in the 10min before the session start!

2/7
3) Session chairs:
use a timer app and also coordinate through the panelist chat with your co-chairs and speakers.

It *MIGHT* happen that you forget to set your timer occasionally, so having backup is great!

3/7
Read 7 tweets

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