Unfortunately it takes too long for me to do the detailed tweet threads I did originally. However, I'll try to hit a few highlights...
In 20I/501Y.V1 (B.1.1.7):
2/15
The Netherlands now has over 300 sequences in 501Y.V1, with clear signs of community transmission.
3/15
Spain has over 250 sequences in 501Y.V1 (most recent in yellow/orange). These include some clear local transmission clusters.
4/15
France has almost 200 sequences in 501Y.V1 now (most recent in yellow/orange. There are some clustering patterns that could suggest local transmission.
5/15
Ireland now has 240 sequences in 501Y.V1. These include clusters of what appears to be local transmission.
6/15
Switzerland now has 112 sequences in 501Y.V1. There are clusters that suggest ongoing local transmission.
7/15
Belgium has almost 200 sequences in 501Y.V1, with clusters that suggest ongoing local transmission.
8/15
Jordan now has 38 sequences in 501Y.V1, including some clusters.
9/15
In 501Y.V2, Switzerland now has 11 sequences, indicating further introductions (pic 1).
France now has 28 sequence, including some large clusters indicating onward local transmission (pics 2 & 3).
10/15
Ireland now has 8 sequences in 501Y.V2, in what looks like 5 separate introductions (pic 1)
The Netherlands now has 22 sequences in 501Y.V2, including some small clusters that may indicate local transmission, or a common exposure source (pics 2 & 3)
11/15
Mozambique has 19 sequences in 501Y.V2, indicating many introductions (pic 1).
There are also sequences from Panama (1), USA (1), Zimbabwe (2), United Arab Emirates (6), Portugal (1), and Luxembourg (6) (pic 2).
12/15
In 501Y.V3 (P.1), we can now see sequences from Italy and the Faroe Islands, alongside the sequences from Brazil and Japan.
13/15
In 20B/S:484K, the USA now has 23 sequences that fall in this variant.
Canada has 11 sequences, all from Ontario, in 20B/S:484K, including some clusters that may indicate onward local transmission or a common exposure source.
14/15
The UK has 15 sequences in 20B/S:484K, including clusters that may represent ongoing local transmission.
There are also sequences from Switzerland (3), French Guiana (mapped to France) (1), Luxembourg (1), Netherlands (2), Ireland (3), and Italy (2) (pic 2).
15/15
Did I miss your country? I'm sorry! But you can check for yourself 😁
Just navigate to the build you're interested in, zoom in to your cluster of interest (you can also 'Filter' to it) then type your country's name into the 'Filter' box so that you only show those tips!
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As well as the major variants of concern, we can spot a couple of other clusters stemming from an 'old' #SARSCoV2 clade, 19B (originating in late 2019).
19A & 19B haven't been around much since early 2020, as they were overtaken by 20A, B & C (which carry D614G).
2/5
However, a few branches of 19B seem to be popping back up again recently.
In the UK, we can see a recent cluster that's acquired S:E484K (along with other mutations in S).
I'm still getting a *lot* of media enquiries - more than I can even respond to! So here's some of my best tips for how to reach out to me to maximise your chances of success (though I imagine they're true of other scientists too!)
1/7
(Just as a disclaimer - even if you use all of these, I can't promise I write back, or that I can say yes. If emails slip off the 'front page' of my inbox they basically fall into a black hole 😬. And sometimes I just cannot take any more interviews, even great ones.)
2/7
1. Please tell me what time zone you're in!
If I want to say 'yes' that means a pencilled-in appointment goes into the calendar immediately - so I need to know when you're likely to be awake. If I have to email you to find out... it might not happen.
3/7
We're hoping to make this a one-stop location to track not only the 'headline' variants, but many different #SARSCoV2 mutations & variants that scientists are keeping an eye on.
Let's take a tour of the site!
1/12
The buttons on the left will take you to pages focused on key variants & mutations, while the menu at the top will help you jump between info pages (FAQ) & pages compiling together information about many variants (ex: Per country).
Let's take a look at the 20A.EU1 page
2/12
Here, we can
- Click through to a dedicated @Nextstrain build for the variant (1)
- See a list of the defining mutations (2)
- Learn a bit about the variant & see links to papers (3)
- See a distribution of 20A.EU1 per country (4)
- See a protein model! (pic 2)
In the 501Y.V1 (B.1.1.7) variant, here are a few of the non-UK countries with new sequences in this variant, including Denmark, Italy, Spain, France, Portugal, Norway, Germany, the USA, South Kora, & Switzerland, & the UAE, Austria, & Czech Republic for the 1st time
2/18
Denmark has 52 new samples (some yellow), showing both separate introductions and further spread of existing local clusters.
As we head deeper into 2021, a gentle reminder to double-check your #SARSCoV2 sequencing scripts for hardcoded '2020's! 😬
We've seen a real uptick recently in sequences with sampling dates in Jan 2020, with divergence that clearly indicates they meant 2021! 📅🤦🏻♀️
"How can we tell?" you might wonder. Actually, these cases are pretty easy to spot!
Let's take a look at two sequences reported as being from Jan 2020 - 1 in green, 2 in blue. (Spoiler, green is the imposter.)
This view is 'date view' where sequences are plotted on the X-axis by the date they were sampled. Then, we try and infer how they're connected using their genetic information - specifically, what mutations they share & don't share.
3 seqs indicated in red are apparently from the hospital. They're coloured by deletion at position 69, which likely caused them to be flagged (501Y.V1 has it too). However, they're related to other sequences in Germany & Turkey, also w the deletion.
We do see the 69/70 deletion has appeared independently previously. While this is interesting, I don't think it increases concern significantly about this group of sequences, which are part of a larger cluster that's been circulating in Germany since spring 2020.