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14 Mar, 18 tweets, 7 min read
Lovecats (acoustic/unplugged) - The Cure
Experimental Love Cat
peta2.com/news/experimen…
Seven subadult cats (aged 6 to 9 months, outbred domestic cats) were intranasally inoculated with 105 PFU of CTan-H. Two animals were scheduled to be euthanized on days 3 and 6 p.i., respectively, to evaluate viral replication in their organs.
ncbi.nlm.nih.gov/pmc/articles/P…
Various Artists - PUSSIFICATION
A Compilation of Experimental Cat Music
Last year, 7 On Your Side exposed a three-decade old practice at a USDA research lab of killing thousands of healthy kittens
scribd.com/document/46016…
With help from @WhiteCoatWaste the kitten experiments were shut down after that investigation.
wjla.com/features/7-on-…
washingtonexaminer.com/news/taxpayer-…
The fact that the USDA has been rounding up pets & other innocent dogs & cats in foreign countries —including at Chinese meat markets condemned by Congress — killing them & feeding them to lab cats here in the States is simply disgusting and unjustifiable"
blog.whitecoatwaste.org/wp-content/upl…
nbcnews.com/politics/polit…
Hualan Chen from Harbin BSL4 now talks about her cat studies which she says:
"We killed the cats on day 3' ha ha ha I mean "euthanised"
caas.cn/cms/web/search…
hvri.ac.cn/en/researchlab…
77 Page Presentation by Hualan Chen
carecprogram.org/uploads/H5N1-A…
x-mol.com/paper/12317541…
A big gap between the fancy taxpayer funded state of the art BSL4 labs and the real serial passage labs down at the local market.
Finishing off with the cannibal cats

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More from @BillyBostickson

Billy Bostickson 🏴👁&👁 🆓 Profile picture
14 Mar
1. Mass Vaccination in a Pandemic - Benefits versus Risks: Interview with Geert Vanden Bossche
sub optimal vaccine immune response leading to viral variance explains why first 3 variants occurred in 3 countries where vaccines were tested, Brazil, UK & SA
2. This interview will make better sense if you read first his open letter "Halt All Covid-19 Mass Vaccination Immediately" (Open Letter to the WHO):
dryburgh.com/geert-vanden-b…
Scroll down for letter to WHO
3. GVB warns of complete disaster:
We must halt all ongoing Covid-19 mass vaccination campaigns as a temporary health benefit to the most vulnerable groups does not justify a public health
disaster of international concern.
dryburgh.com/wp-content/upl…
Read 9 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
13 Mar
To clarify
The "scientist" who knows about WIV deleted databases, who blocked anyone who asked about them for months, who admits he knows what is in them, who knows all about WIV work that could have led to a lab leak, is the guy put in charge of 2 "investigations" (Lancet & WHO)
The PDF of the above 2017 letter describing their work (as referenced by daszak)
link.springer.com/content/pdf/10…
The PPT:
static-content.springer.com/esm/art%3A10.1…
Read 9 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
7 Mar
Compilation Thread on ORF8 ORF8 or Fate?
1. ZLS (2018):
"Considering the variability of orf8 in bats, civets and humans, investigating the function of orf8 is a priority, particularly the contribution of these different variants to viral pathogenesis."
2. ORF8 or Fate?
"In addition to receptor binding, proteolytic cleavage of S & potentially other mutations that affect virion & trimer stability may be important for virus transmissibility in different hosts, & these factors need to be studied further."
nature.com/articles/s4157…
3. ORF8 or Fate?
Overwhelmingly these viruses had mutations or
29 deletions in ORF8 - associated with reduced replicative fitness of the virus.. may be associated with host adaptation
via @CarltheChippy
382-nt deletion during early evolution of SARS-CoV
biorxiv.org/content/10.110…
Read 30 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
7 Mar
(A and B Sequence & structural properties of insert PRRA (D) Sequence similarity between close neighborhood of PRRA insert, neurotoxin motifs Y674QTQTNSPRRAR685, homologous to Ophiophagus (cobra) & Bungarus genera & 3 RABV strains & HIV-1 gp120 superantigenic motif in last row
Study on Ribonuclease From Snake Venom of Zhejiang Cobra (Naja naja atra)
ZHANG Gon-jin
Wuhan Institute of Virology,Academia Sinica
zoores.ac.cn/article/id/1281
Read 4 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
5 Mar
1. Within-Run Cross-Contamination in Deep Sequencing Applications on the Illumina MiSeq

croiconference.org/abstract/withi…
2. Common Contaminants in NGS
Until an effective strategy is found to eliminate contaminants from sequencing runs, novel sequences detected in such runs should be scrutinized by qPCR analysis to ensure they originate from specimens rather than the lab
journals.plos.org/plosone/articl…
3. From trash to treasure: detecting unexpected contamination in unmapped NGS data
bmcbioinformatics.biomedcentral.com/articles/10.11…

Here we propose DecontaMiner, a tool to unravel the presence of contaminating sequences among the unmapped reads
Read 7 tweets
Billy Bostickson 🏴👁&👁 🆓 Profile picture
5 Mar
@Daoyu15 @franciscodeasis @spectropan @ResearchGate @TheSeeker268 STRANGE TO SEE THIS IN Wuhan Hu-1
Wuhan seafood market pneumonia virus isolate Wuhan-Hu-1
Clostridium_difficile
Bat coronavirus BM48-31/BGR/2008
Human coronavirus 229E
@Daoyu15 @franciscodeasis @spectropan @ResearchGate @TheSeeker268 A Bulgarian Bat Virus mentioned in Lam et al:
Identification of 2019-nCoV related coronaviruses in Malayan pangolins in southern China
biorxiv.org/content/10.110…
@Daoyu15 @franciscodeasis @spectropan @ResearchGate @TheSeeker268 Bat coronavirus BM48-31/BGR/2008

Drosten

ncbi.nlm.nih.gov/nuccore/NC_014…
and
ncbi.nlm.nih.gov/Taxonomy/Brows…
Read 10 tweets

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