If you're working on spatial transcriptomics, I think you'll find @LambdaMoses' "Museum of Spatial Transcriptomics", which analyzes the field via its metadata, to be an incredibly useful resource. biorxiv.org/content/10.110… 1/11
The museum is organized as a main paper that provides an overview of a book (i.e. the Supplementary Material) which is based on a database of papers in the field compiled by @LambdaMoses. First the database... docs.google.com/spreadsheets/d…

It contains several hundred papers. 2/11
To undertake a comprehensive study of the field, @LambdaMoses read all these papers carefully, starting with "prequel" literature to establish historical context. The database has detailed metadata including a summary of each paper. This timeline is just of the prequel. 3/11
In the book, you can find a detailed analysis of the metadata, which reveals interesting trends and dynamics in the field. For example, the prequel chapter is here: pachterlab.github.io/LP_2021/preque… 4/11
The analysis is not just qualitative. @LambdaMoses performs an analysis of numerous aspects of the literature. E.g., here is a plot showing the particular organs (and # of genes assayed) in whole-mount in situ hybridization atlases, i.e. (WM)ISH. 5/11
All of these analyses are easily producible, by running R code that processes the database. When the databases is updated, the book is updated. The code is all here github.com/pachterlab/LP_… and can be run on the Rstudio cloud. 6/11
Some of the results are astounding. Interested in correlations between topics (in spatial transcriptomics)? Here it is: 7/11
Want to know which methods have been widely adopted, versus technologies that are mostly used in-house? Here it is for smFISH based techniques (there are similar figures for other methods). 8/11
If you're interested in the data analysis aspect of spatial transcriptomics, the book contains basic analysis, for example of programming languages used. 9/11
But much more interesting is the in-depth analysis of computational methods that I believe is the most detailed to date in the field. See chapter 7. pachterlab.github.io/LP_2021/curren… 10/11
This is truly a "museum of spatial transcriptomics" and we hope that beyond its utility for spatial transcriptomics, the approach serves as a proof-of-principle that could be applied to study other fields. I am truly in awe of what @LambdaMoses has accomplished. 11/11

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More from @lpachter

13 Apr
Yesterday I posted a piece about @OrchidInc's polygenic embryo selection. I thought, based on a press release I read, that they were the first company to undertake polygenic embryo selection. 1/ liorpachter.wordpress.com/2021/04/12/the…
The press release started w/ "Orchid, the first preconception system to quantify how a couple's genetics impacts their future child's health, today announced a $4.5M seed round..". It went on to describe the company's polygenic embryo selection product. 2/ prnewswire.com/news-releases/…
I naïvely assumed that Orchid is the first company to embark on polygenic embryo selection, but TIL that is not the case. In fact, more than two years ago, an article in @TheEconomist discussed myome. 3/
Read 8 tweets
31 Mar
I have a few things to say about this tweet attacking @mbeisen and subtweeting me. Specifically, I want to talk about cancel culture gone mad... 1/14
In September I wrote a blog post reciting several false #covid19 claims and predictions made by Levitt over the course of the pandemic. That is not an "ad hominem attack". I reported Levitt's claims (with references). liorpachter.wordpress.com/2020/09/21/the… 2/14
Levitt, for his part, has responded to criticism of his failed predictions with non-sequiturs about attacks on free speech. 3/14
Read 14 tweets
29 Jan
This past week my lab published 4 @biorxivpreprint papers in applied math (biorxiv.org/content/10.110…), biology (biorxiv.org/content/10.110…), bioinformatics (biorxiv.org/content/10.110…), and instrumentation (biorxiv.org/content/10.110…). They were possible thanks to reproducibility... 1/
There is a lot of focus on the importance of reproducible science for facilitating replication of published research. That's all good, but reproducible science has another benefit: when adopted by a group it is an incredible accelerant for research *in that group*. 2/
Consider the paper we wrote on whole animal multiplexed #scRNAseq. The @GoogleColab notebooks Tara Chari wrote for the analyses were a monumental effort, but she did not start from scratch. 3/
Read 8 tweets
29 Jan
Happy to announce a new open-source instrument from our lab, this time a low-cost, scalable, and automated fraction collector for fluidics applications. Beautiful work by @sinabooeshaghi & @annekylosaurus, with @lioscro and @JaseGehring. 1/
biorxiv.org/content/10.110…
The design is simple and elegant. A single motor drives the shaft of the tube rack, which is coupled to the dispenser arm via a spiral track. This ensures both rotate in tandem. 2/
The device is easy to 3D print and build, and can be assembled from off-the-shelf parts in less than an hour for $67.02. This low cost, and the straightforward assembly, is possible thanks to the design around a single motor. Amazing work by @annekylosaurus & @sinabooeshaghi. 3/
Read 12 tweets
28 Jan
Universal Health Services @UHS_Inc is the largest facility-based behavioral health provider in the country. Its mission statement includes "To provide..healthcare services that..INVESTORS seek for long-term returns."

Hard to believe right?

This is straight from their "mission statement" website, capitalization and all... uhsinc.com/mission-statem…
Last year UHS settled with the DOJ for $122 million. complianceweek.com/regulatory-enf…
Read 5 tweets

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