1. The Yeast is Red and Many are Dead Thread
Sub Yeast Thread 1
2. via @ydeigin and @franciscodeasis
it’s a very disturbing paper if its findings are verified: not only SARS2 and RaTG13 have signs of genetic manipulation in yeast but SARS1 too.
WIV is known to have eyed yeast reverse genetic systems with interest (see attached WIV thesis
3. The Author has published quite a few interesting papers with 1870 citations.
One on the Association between influenza vaccination rates and SARS-CoV-2 outbreak infection rates in OECD countries
4. Random Yeast Papers - WIV
Construction and Rescue of a Functional Synthetic Baculovirus
5. Honours in Yeast (08/2020) - Yeast Virus Cookbook
Every Tweet is one Tab Less ;)
In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline
and pdf:
6. Yeast containing SARS-COV- 2
“The exciting thing about the yeast is that . . . it’s fast,” says expert Susan Weiss
Scientists Clone SARS-CoV-2 Genome with Quick Yeast-Based Method (May 2020)
7. Dr. Weiss's Magic Yeast

What this means is that instead of having to reconstruct the viral DNA first before introducing it into cells, you instead “put all these fragments in the yeast and it magically puts them together,” says Weiss.

transformation associated recombination (TAR)

yeast artificial chromosome (YAC)

Data Not Available (DNA) Don't believe me? Ask WIV!
9. Amazing Recombination Capacity

“The capacity to recombine things to a YAC by themselves is just amazing,” says Jores.
10. In Vitro Ligation

Refers to this UTMB paper:

An Infectious cDNA Clone of SARS-CoV-2


Note: Pei Yong Shi and James Le Duc
11. Method from above UTMB paper

An Infectious cDNA Clone of SARS-CoV-2

12. One Week Is All It Takes

Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform

13. Nice Clear Layman's Explanation (Thank God)

Researchers develop synthetic novel coronavirus clones

14. Well illustrated background reading

Billy Recommends: More Yeast with your Whining!

Synthetic genome engineering forging new frontiers for wine yeast

15. Virus Like Particle Vaccine Manufacture from Yeast
(Illustrated Master's Thesis) 2020
16. Synthetic viruses—Anything new?

asks Volker Thiel in 2018

The generation of synthetic poxviruses as described by Evans and colleagues and cloning of synthetic DNA using TAR in yeast are illustrated

17. Yeast-based synthetic genomics platform to genetically reconstruct RNA viruses exist.

How much were they used by Chinese Researchers in the years leading up to the pandemic is an interesting research project.
18. WIV Research (2018) - Yeast

Advances and challenges in enveloped virus-like particle (VLP)-based vaccines

Shiyu Dai, Hualin Wang, Fei Deng

State Key laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan

19. Time for a break!

Unroll the Yeast is Red @threadreaderapp
20. Revenge of Yeast
a WIV Thesis that @TheSeeker268 found recently is by Lei Ping Zeng (2017) & it mentions WIV's interest in yeast recombination methods

Reverse Genetic System of Bat SARS-like Coronaviruses & Function of ORFX
21. Zeng Translated
(by @TheSeeker268 & @franciscodeasis )
Only 2 references to yeast recombination but obviously on their radar back in 2017.
22. Across ocean & back in time to Wizard of UNC

Thanks Jim Haslam @jhas5

Yeast Based Small Molecule Screen for Inhibitors of SARS-CoV

23. I am getting so sick of Yeast!
I will just dump yeast infected papers here for others to vomit over
Yeast for virus research (2017) Richard Yuqi Zhao
24. Enough Yeast!
2007: Saccharomyces cerevisiae: a versatile eukaryotic system in virology
2010: A High-Throughput Approach for Studying Virus Replication in Yeast
25. I will finish with yeast until i get more yeastinspiration

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More from @BillyBostickson

11 Jun
The National Natural Science Foundation of China

1. Jian Kang, a retired associate professor from New York Medical College, pointed out the following evidence supporting the Laboratory leak of the SARS-COV-2 Virus Image
2. The National Natural Science Foundation of China provided zero funding for the research on the origin and intermediate host of the SARS-Cov-2 virus, while the funding for research on the origin and intermediate host of the SARS-1 virus was nearly 2 million. Image
3. The main supporting evidence for a natural origin of SARS-COV- 2 is finding the intermediate host.

From the perspective of profit & loss, the biggest beneficiary of finding an intermediate host is China, to show that the virus comes from nature, not a man-made disaster. Image
Read 14 tweets
9 Jun
It's Fun Time with Yeast DNA! New paper!
Differential enrichment of yeast DNA in SARS-CoV-2 and related genomes supports synthetic origin hypothesis
46 Pages, Posted: Andreas Martin Lisewski
Jacobs University, June 5, 2021
I will be reading this now
Crikey! SARS 1 SARS COV 2 and RaTG13!
Diagram 1
Read 13 tweets
8 Jun
1. William Karesh - What we know
@Bobby_Network has just made an interesting thread on Bill Karesh and the fingers he has in many pies

However, there is more that we know and researched before which I will add here as a reference thread.
3. The "secret handshake" club
Michael Callahan was in Nanjing in December 2019 and would know more about Agency funding via "United Therapeutics"
Daszak dealt more with DTRA, DARPA & DOD via Jonathan Epstein & Bill Karesh for US virus collection project.
Read 15 tweets
5 Jun
1. Someone on Linkedin asked this interesting question:

Anyone care to speculate why Fauci FOIA emails to non-government individuals (especially to Zuckerberg and Daszak) are heavily redacted with these codes?

(b)(4), (b)(6), (b)(7)(A)
2. (b)(4)

Reveal information that would impair the application of state-of-the-art technology within a U.S. weapon system
3. (b)(6)

Reveal information, including foreign government information, that would cause serious harm to relations between the United States and a foreign government, or to ongoing diplomatic activities of the United States
Read 22 tweets
3 Jun
Interesting revelations about the "can of worms" Definitely worth a read, but a completely false history of #DRASTIC
Obviously @KatherineEban was misinformed
Best quote was from @dasher8090
Good to see Thomas DiNanno's numantine struggle at the heart of US bureaucracy recognised
What a difference a year makes vanityfair.com/news/2020/05/w…
Read 26 tweets

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