The 2nd preprint from G2P-Japan is out at @biorxivpreprint !
We revealed that the SARS-CoV-2 delta variant (B.1.617.2 lineage) is highly fusogenic and #P681R mutation, a hallmark in the B.1.617 lineage, is its determinant. Please retweet 1/3
In this study, we demonstrated that #P681R mutation in the SARS-CoV-2 spike protein enhances and further accelerates SARS-CoV-2 spike-mediated cell-cell fusion. 2/3
Photo: Syncytia formed by SARS-CoV-2 infection in VeroE6/TMPRSS2 cells. Note that B.1.617.2 is the delta variant, and B.1.617.1 is a variant emerged in India. B.1.1 is a prototype. Referred from Figure 2B of our preprint. 3/3
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A new preprint "An emerging SARS-CoV-2 mutant (='the California variant', B.1.427/429) evading cellular immunity and increasing viral infectivity", from The G2P-Japan consortium, organized by my lab, is out at @biorxivpreprint. Please RT 1/7 biorxiv.org/content/10.110…
During the current SARS-CoV-2 pandemic that is devastating the modern societies worldwide, many variants that naturally acquire multiple mutations have emerged. Emerging mutations can affect viral properties such as infectivity and immune resistance. 2/7
The sensitivity of naturally occurring SARS-CoV-2 variants to 'humoral immunity (=neutralizing antibodies)' has recently been investigated. However, the impact of viral mutations to human leukocyte antigen (HLA)-restricted 'cellular immunity' remains unaddressed. 3/7
Please notice: we revealed that SARS-CoV-2 ORF3b, one of the most different genes compared to SARS-CoV, strongly hampers human type I IFN activation. Its inhibitory activity is stronger than the SARS-CoV ortholog and influenza A virus NS1. #COVID19 1/7 biorxiv.org/content/10.110…
Our findings may explain the poor IFN responses in #COVID19 patients and SARS-CoV-2-infected cells compared to SARS-CoV- and influenza A virus-infected cells, recently reported by @DBM003@virusninja and so on, are attributed to the remarkable ability of SARS-CoV-2 ORF3b. 2/7
Additionally, we revealed that the ORF3b genes of SARS-CoV-2-related viruses in bats and pangolins are very similar to that of SARS-CoV-2 and possess the activity to inhibit IFN activation. 3/7