For those using @ArticNetwork Midnight for #SARSCoV2 sequencing: analysis of the available data (55 genomes) showed that 100% were correctly identified as Omicron. We’ve observed 1 amplicon dropout out of 29. This is outside of the S gene & does not impact correct lineage ID 1/4
Whilst the amplicon dropout does not affect identification of the Omicron variant, the Midnight kit will be revised to include an additional primer to improve amplification efficiency of Omicron Amplicon 28. We expect this to be available in 2-4 weeks 2/4
We are again thankful to @freed_nikki and the entire @ArticNetwork community for quick and open data sharing so that we can continue to provide high quality solutions for #SARSCoV2 surveillance around the globe 3/4
We are available to support and supply any users wishing to sequence #SARSCoV2, or to start using nanopore — you can find information here nanoporetech.com/covid-19
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Opening the Nanopore Community Meeting Tech Update is James Clarke, valiantly speaking through a bad, bad cold. We'll be live tweeting in this thread, with Stu Reid and Rosemary Dokos up next #nanoporeconf
JC: @nanopore accuracy has come a long way. Right now, in just 3-4 seconds, I can pass 1,000 bases of a **single molecule** of DNA through a hole, and know its sequence with over 99% accuracy. That’s better than Q20, by looking at a single molecule - once #nanoporeconf
JC: Beyond single molecule, you can of course use multiple strands in a pile-up. We’ve achieved Q50 consensus accuracy on bacterial genomes, from 20X coverage. #nanoporeconf
Coming up in a few minutes, Clive Brown @the_taybor, with an update entitled “Nobody Expects the Strandish Exposition”. Follow the talk live in this thread #nanoporeconf
CB: Our goal is to enable the analysis of anything by anyone, anywhere. Here are some examples of global use: nanoporetech.com/portable-seque…
CB: As a reminder, this is how nanopore sequencing works. #nanoporeconf
Opening the tech update at the Nanopore Community Meeting, from Oxford @Nanopore, James Clark, VP platform technology #nanoporeconf
JC: a reminder that our goal is to enable the analysis of anything, by anyone, anywhere #nanoporeconf
JC: Looking at about the key qualities of nanopore sequencing: high accuracy data, with high yields, information-rich data, a versatile platform that scales from portable to ultra-high throughput #nanoporeconf
First up, James Clarke with an introduction to @nanopore sequencing. More than 1,000 publications use nanopore sequencing nanoporetech.com/resource-centre, and we estimate that ~1.7 petabases of nanopore sequence has been generated to date #nanoporeconf
JC: Here’s how nanopore sequencing works. Library prep straightforward: add an adapter to each end of the sample DNA molecule, containing leader, motor protein, motor stall, tether #nanoporeconfyoutube.com/watch?v=RcP85J…
Dan Turner @DanTurnez: an overview of LamPORE, the new nanopore based assay for the detection of SARS-CoV-2 at #nanoporeconf. A thread:
DT: Why is the LamPORE, sequencing-based assay useful for SARS-CoV-2 detection? We think about bottlenecks: the ability to analyse very small or very large numbers of samples with a small footprint, rapid results, and efficient, precise analysis of known pathogens #nanoporeconf
DT: With RT-PCR, performing a test in duplicate or triplicate is common. If sequencing is used as an output, the precise nature of the readout means that a 96 well plate can do the full 96 samples #nanoporeconf#LamPORE