Thread by @veeslerlab

The peer-reviewed version of our manuscript describing the structures of the #SARS_CoV_2 #OmicronVariant spike bound to the clinical S309 antibody (sotrovimab parent) and receptor-binding domain bound to ACE2 (and S309/S304) is now available here.

science.org/doi/10.1126/sc…

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Here is the original thread describing this study

https://twitter.com/veeslerlab/status/1476953852767203335

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During the reviewing process, we added binding data to show that most clinical antibodies have reduced or abrogated binding to the #OmicronVariant spike trimer relative to Wuhan-Hu-1 spike

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In agreement with the receptor-binding domain binding data that were already present in our @biorxivpreprint preprint

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These data reflect the abrogated neutralization from these antibodies except for S309 (~3x drop) and COV2-2130/COV2-2196 (10-200x drop), as shown here

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nature.com/articles/s4158…

We show that the S371L/S373P/S375F mutations enhance conformational dynamics of this region of the receptor-binding domain and hinder recognition of some antibodies targeting this site, suggesting these mutations arose (at least in part) to promote immune evasion.

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Thanks again to @Dr_MattMcCallum Nadine Czudnochowski @SamK_Zepeda @johnbowenbio @coronalexington @CrollTristan @DavideCorti6 Gyorgy Snell & many others!

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