Thread by @AutumnSkyeIvy

It’s here! 🎉 Our study reveals both new and known #geneexpression programs involved in hippocampal memory following #adolescent #exercise, and also investigates two #epigenetic regulatory mechanisms (open access @CommsBio) 1/

urldefense.com/v3/__https://r…$

We asked which genes are regulated after aerobic exercise (voluntary wheel running) in adolescent #hippocampus using a transgenic mouse & technique we developed for simultaneous sequencing of the #epigenome and translatome in excitatory #neurons. 2/

Using our “SIT”protocol from a single hippocampus (Simultaneous INTACT and TRAP; yes we used “SIT” in running mice!) found high correlation (Spearman’s) when compared to traditional INTACT/TRAP in separate samples. Protocol here: 3/

…rentprotocols.onlinelibrary.wiley.com/doi/full/10.10…

Panther GO and GSEA analyses were applied to implicate molecular and biological functions of exercises’ impact on hippocampal genes, revealing some interesting regulatory networks in IPA analysis. 4/

We correlated ⬆️and ⬇️translating mRNA with occupancy or removal of epigenetic mods H4K8ac and H3K27me3 🧬 Many genes had altered epigenetic mods after exercise. Maybe they are “primed” by exercise for expression when needed; I.e., when learning something new…. 5/

So we tested this. Mice underwent hippocampal learning, and during memory consolidation, ~70ish genes seemed to be primed by exercise for new expression and were unique to exercised mice that learned! IPA implicates the possible functions of these genes. 6/

These data suggest new, exercise-based strategies for therapy or exercise regimens to target/activate gene programs for learning and attention (think autism, or stress induced cognitive impairments during early life) stay tuned for follow up experiments! 7/

This was a huge team effort by co-first authors & trainees Anthony Raus, Dr. Tyson Fuller and Nellie Nelson. Also have to acknowledge support from @NINDSfunding K12, @RWJF #AMFDP, and UCI ICTS and Conte Center. We’re excited for next steps! *end

Found out initial link was broken- here’s the article:

nature.com/articles/s4200…