Thread by @AutumnSkyeIvy

Finally published! 🎉 reposting d/t broken link in first thread:

Early-life exercise primes the murine neural epigenome to facilitate gene expression and hippocampal memory consolidation | Communications Biology nature.com/articles/s4200…

Our study discovers both new and well-known #geneexpression programs involved in #hippocampal memory following #adolescent #exercise, and also investigates two #epigenetic modifications in these mechanisms (open access @CommsBio)

We asked which genes are altered following aerobic exercise (voluntary wheel running) in adolescent hippocampus, using a transgenic mouse & technique we developed for simultaneous sequencing of the epigenome and translatome in excitatory neurons.

Using our “SIT”protocol from a single hippocampus (simultaneous INTACT and TRAP; yes we used “SIT” in running mice!) found high correlation (Spearman’s) when compared to traditional INTACT/TRAP in separate samples. Protocol here:

…rentprotocols.onlinelibrary.wiley.com/doi/full/10.10…

Panther GO and GSEA analyses were applied to implicate molecular and biological functions of exercises’ impact on hippocampal genes, revealing some interesting regulatory networks in IPA analysis.

We correlated ⬆️ and ⬇️ translating mRNA with occupancy or removal of epigenetic mods H4K8ac and H3K27me3. Many genes had altered epigenetic mods after exercise. Maybe they are “primed” by exercise for expression when needed; I.e., when learning something new….

So we tested this. Mice underwent hippocampal learning, and during consolidation 70ish genes seemed to be primed by exercise for new expression and were unique to exercise mice that learned! IPA implicates the possible functions of these gene programs.

These data allow discovery of new, exercise-based targets for therapeutic strategies for the improvement of learning, memory or attention issues in models of cognitive problems (think autism, stress induced cognitive impairments during early life) stay tuned!