Thread by @EricTopol

What is we were immunoassay-guided for who to give vaccines, whether the vaccine was effective, when 1 dose was enough?
Just now, 10 days after my 2nd dose vaccine, I had a quantitative multiplex immune response panel, via finger stick, taking <15 mins, watched in real-time 1/

I'll go through the steps but the bottom line is that I fortunately had very high levels of IgG to #SARSCoV2 RBD (receptor binding domain), the S1 of there spike protein and the S2 portion of the spike protein 2/

Steps
1. My finger stick blood into a capillary tube
2. Put on a dedicated, reusable chip
3. Put into the machine
4. Assays quickly running /3

In real-time I am watching my IgG to RBD go off the chart
(top, draw blue line), 7 minutes in /4

And after buffers stay up there /5

I had a paired venipuncture sample run and the results were very similar /6

Note that I have IgG antibodies to 2 common cold coronaviruses (HKU1, NL63) that I didn't have when I had the same panel back in March (thread about that here)/ 7

https://twitter.com/erictopol/status/1243652959923097602?lang=en

The lack of a nucleocapsid IgG response is in keeping with the S-protein target of the vaccines

Let me make clear that I have no COI, no relationship with Genalyte /8

If this could scale to millions of test/ day, such an assay panel could used to assure vaccine efficacy, defer vaccines for people who've had covid-19, and test single dose strategies. ~ 70 million Americans have had covid, the assay picks up infections > 10 months /9