when planning experiments, I make a sort of "summary" sheet beforehand listing reaction conditions, etc. for reference later (such as when I'm exhausted from the experiments!) & include a "goals" section so I always keep in mind *why* I'm doing all that hard work #labtips n/n
I have a "storyboard" Adobe Illustrator artboard where I lay out my key figures & how they tell the story of my research - what's the point of each? anything missing? and wow - you've really done a lot! (note: free alternatives to Illustrator are Inkscape & GIMP) #labtips n/n
Add SDS Loading Buffer, NOT ladder, to your gel samples... 🤦♀️ #labtips n/n
80% glycerol. Do it. So much easier to pipet than 100%. If you're freezing a protein in a high concentration glycerol, you can make the solution in your buffer. And instead of trying to measure by pipetting, add it to liquid in a graduated cylinder. #labtips n/n
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I don't know if these would be helpful to anyone, but I found a bunch of protein crystallization diagram graphics I'd made a long time ago that were embarrassingly ugly & and "refreshed" them - you can find them all here: bit.ly/crystallizingp…
Say you want to study how a protein works... What can you do? A thread on recombinant protein expression and purification (with links to more) 1/
The first step's molecular cloning, where you take the genetic instructions for making that protein (cDNA) & stick them into another piece of DNA (such as a plasmid) that you can use as a "vector" (vehicle) to get the instructions into expression cells bit.ly/molecularcloni… 2/
A common form of molecular cloning uses restriction enzymes to "cut and paste" the DNA from one place to another 3/ bit.ly/restrictionenz…
Do you listen to your spectrograph peaks when they try to speak? Or just look at highest 1 & assume purification's done? Peakiest peak *might* tell concentration - but rest can reveal contamination! 👉 bit.ly/2NblGc2 #366DaysOfScience#biochemistry#scicomm#realtimechem