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Mar 6 • 4 tweets • 4 min read
I don't know if these would be helpful to anyone, but I found a bunch of protein crystallization diagram graphics I'd made a long time ago that were embarrassingly ugly & and "refreshed" them - you can find them all here: bit.ly/crystallizingp… ImageImageImageImage
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More from @biochem_bri

Brianna Bibel Profile picture
Mar 6, 2021
Say you want to study how a protein works... What can you do? A thread on recombinant protein expression and purification (with links to more) 1/
The first step's molecular cloning, where you take the genetic instructions for making that protein (cDNA) & stick them into another piece of DNA (such as a plasmid) that you can use as a "vector" (vehicle) to get the instructions into expression cells bit.ly/molecularcloni… 2/
A common form of molecular cloning uses restriction enzymes to "cut and paste" the DNA from one place to another 3/ bit.ly/restrictionenz…
Read 41 tweets
Brianna Bibel Profile picture
Jan 29, 2021
when planning experiments, I make a sort of "summary" sheet beforehand listing reaction conditions, etc. for reference later (such as when I'm exhausted from the experiments!) & include a "goals" section so I always keep in mind *why* I'm doing all that hard work #labtips n/n
I have a "storyboard" Adobe Illustrator artboard where I lay out my key figures & how they tell the story of my research - what's the point of each? anything missing? and wow - you've really done a lot! (note: free alternatives to Illustrator are Inkscape & GIMP) #labtips n/n
Add SDS Loading Buffer, NOT ladder, to your gel samples... 🤦‍♀️ #labtips n/n
Read 4 tweets
Brianna Bibel Profile picture
Jan 24, 2021
Just in case you need some splice in your life... 1/8: gene to protein overview Image
2/8: pre-mRNA to mRNA Image
3/8: alternative splicing Image
Read 8 tweets
Brianna Bibel Profile picture
Jan 7, 2020
Spectroscopy allows us to spy on molecules invisible to the naked eye. Through a cuvette the spectrophotometer allows us to peer and measure concentrations with the help of Beer! 👉 bit.ly/2N4nzXE
#366DaysOfScience #biochemistry #scicomm #realtimechem
The Beer Lambert law (Beer’s law) allows you to convert between concentration (c) of a dissolved thing (solute) & absorbance of light. Don't have a lot - use a NanoDrop so the UV-Vis fun don't stop! bit.ly/beerslawexplai…
#366DaysOfScience #biochemistry #scicomm #realtimechem
Do you listen to your spectrograph peaks when they try to speak? Or just look at highest 1 & assume purification's done? Peakiest peak *might* tell concentration - but rest can reveal contamination! 👉 bit.ly/2NblGc2
#366DaysOfScience #biochemistry #scicomm #realtimechem
Read 360 tweets
Brianna Bibel Profile picture
Oct 5, 2018
After a jam-packed 2 days at #SciOut18🌪(thx @ASBMB & @rockedu_!) it was time to reflect! 🤔 Reflection is so important, but it can be hard to find ⏰ to really just think! 💭 Some of my thoughts 👉 instagram.com/p/BokNO_NhihF/
#365DaysOfScience #biochemistry #scicomm #sciout #science person looking thoughtful in front of mirrornotebook pagenotebook pagenotebook page
What’s inside? 🧐 It’s FORMAMIDE! 🤗 Formally removing RNA’s form with formamide! 🤓 FORMAMIDE in loading buffer breaks up & replaces the bonds between bases holding RNA's shape together 👇
instagram.com/p/BomwR32B4MX/
#365DaysOfScience #biochemistry #scicomm #lifeinthelab #science person looking through safety goggles with formamide drawn on themformamide RNA denaturant explanation
Bring on the heat! 🔥 To help denaturants get to the huddling hydrophobic bases, we add heat ♨️ 👉 gives molecules more energy so they can “pull apart” more easily ⏩ easier access 👍 More 👇
instagram.com/p/Boo2yazBDDi/
#365DaysOfScience #biochemistry #scicomm #lifeinthelab #science person putting tube in heating block
Read 308 tweets

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