Thread by @I_mitochondria

Risk of exposure to babies being fed by mothers vaccinated with SARS-CoV-2 experimental vaccine-mRNA

There can be no assurances of safety, with so many unknowns

hartgroup.org/risk-to-babies…

It is being claimed that:

1.Human breast milk does not contain SARS-CoV-2 vaccine-mRNA; and

2.That if there were any vaccine-mRNA in breast milk, it would not survive the nursing infant’s digestive tract.

facebook.com/10001433352474…

Current claims regarding the safety of the vaccine-mRNA itself are not supported by robust evidence.

In a recent preprint, Low et al. (2021) detected SARS-CoV-2-COVID19 (SARS2) vaccine-mRNA in human breast milk.

medrxiv.org/content/10.110…

n=10 lactating healthcare workers, nursing their infants
4 breastmilk samples collected/participant

Sample taken:
1: pre 1st dose of Pfizer/BioNtech experimental vaccine,
2: 1-3 days post,
3: 7-10 days post 1st dose,
4: 3-7 days post second dose of experimental mRNA vaccine.

We do not know if the samples represent the foremilk or hindmilk, which may impact results.

Authors detected vaccine-mRNA presence in 4/40 samples, giving the impression of a low rate of +ve detection, as 90% of samples were vaccine-mRNA free, implying presence was minimal/rare

Looking at the study data:

The 4+ve of the 40 samples, came from 3 participants out of the 10

This means 30% of the mothers tested positive to having vaccine-mRNA in their breast milk.

Put another way:

3 out of 10 babies are being exposed to experimental vaccine-mRNA.

This number may likely be greater, given nursing infants often nurse more than five times a day and the only 4 samples of breast milk collected per participant, those samples were taken over several weeks.

The authors do not indicate if they collected foremilk or hindmilk and we do not know which stage of milk would have more extracellular-vesicle (EVs) containing vaccine-mRNA, nor if there would be EV free vaccine-mRNA (mRNA not inside of a vesicle).

If the vaccine-mRNA is in the more fat-rich hindmilk, while the samples collected were from the foremilk this would have increased the likelihood of missed detection.

A better study would have taken at minimum 2 samples everyday, one sample being foremilk and one being hindmilk, for at least 28 days post 1st and again post 2nd dose of experimental vaccination:

2 pre 1-fore/1-hindmilk
1 foremilk/1 hindmilk/day for 28days post 1st and 2nd dose

This investigation claimed the level of vaccine-mRNA detected in human breastmilk was very low, the highest concentration at 2ng/mL.

A nursing infant may on average drink 100mL of breastmilk per feed, resulting in a baby potentially exposed to 200ng of vaccine-mRNA in one meal

200ng/100mL breast milk/feed.

Now multiply that by five feeds in a day, bringing it up to 1,000ng/feed

1 microgram in a day.

Assuming baby feeds at least 5 times per day (potentially more), this clearly increases the risk of breastmilk derived vaccine-mRNA exposure.

It is claimed:

Any vaccine-mRNA present in breastmilk, consumed by the infant, will be readily digested.

However, this is an unproven claim, as it has not been tested and is something that should be investigated in animal studies before embarking on experimenting on babies.

The vaccine-mRNA is transported in lipid nanoparticles designed to mimic human extracellular vesicles (EVs).

There is extensive research demonstrating that EVs in milk survive the human digestive tract…

ncbi.nlm.nih.gov/pmc/articles/P…

…and that human breast milk EVs are readily taken up into the vascular system and more specifically bioaccumulate in the brain.

Milk-derived exosomes and metabolic regulation
core.ac.uk/download/pdf/3…

Milk exosomes: beyond dietary microRNAs
genesandnutrition.biomedcentral.com/articles/10.11…

MicroRNAs and mRNA have been detected in human breast milk, and shown to survive passage through the stomach, protected from enzymatic digestion and acidic conditions, maintaining their biologically active capabilities.

sciencedirect.com/science/articl…

nature.com/articles/s4159…
sciencedirect.com/science/articl…
sciencedirect.com/science/articl…
karger.com/Article/Abstra…
frontiersin.org/articles/10.33…
mdpi.com/2072-6643/13/4…
ncbi.nlm.nih.gov/pmc/articles/P…
https://t.co/D6j72kfQES
academic.oup.com/advances/artic…

Furthermore, RNA has been found in a highly stable vesicle-free form in the bloodstream, complexed with proteins such as Argonaute2 (Ago2) which protect them from digestion by RNase enzymes.

ncbi.nlm.nih.gov/pmc/articles/P…

This challenges the claim that the vaccine-mRNA is rapidly degraded.

nature.com/articles/s4158…

How do we know that fragmented versions of these non-natural enhanced mRNAs are not biologically active, much in the same way as miRNA and siRNA

sciencedirect.com/science/articl…

It is clear there is still so much unknown, which means there can be no assurances of safety.

Extracellular, EV encapsulated, or fragmented vaccine-mRNA, if not digested, will like be recognised as DAMPS (damage associated molecular patterns) by the innate immune system, and initiate inflammatory signalling cascades.

This clearly would not be good for a nursing baby.

We have no idea if it will contribute to gut issues, it has already been shown that breastmilk EVs and miRNAs bioaccumulate in the brain, and those that do not, alter gene regulation in the gut.

ncbi.nlm.nih.gov/pmc/articles/P…

It is likely that the vaccine-mRNA will be found in EVs, however, we do not know if fragmented and/or EV-free parts could make it into the plasma and breast milk, potentially protected by complexing with proteins such as Ago2.

Obviously, because there are so many unknowns, this is exactly why fully completed wide ranging studies are required to be completed before subjecting humans to risk from unknown harms.

A common mistake many people are making, is equating natural mRNA properties with the SARS-CoV-2 injection mRNA. The vaccine-mRNA has been modified to a form never before seen in nature, and manipulated to ensure stability and delay enzymatic digestion.

One of the methods is the uridine nucleotides are not the type found in natural mRNA, they have been substituted with N-methyl-pseudo-uridines.

This methylation was done to prevent the vaccine-mRNA from being easily broken down, altering digestion...

ncbi.nlm.nih.gov/pmc/articles/P…

...enzymes ability to recognise and access cleavage sites, that is part of the degradation process, stabilising the vaccine-mRNA against digestion, ensuring longer survival.

ijvtpr.com/index.php/IJVT…

To further increase stability of the vaccine-mRNA, a cap and tail modification was added.

7- ethylguanosine “cap” at the 5’ end of the vaccine mRNA and a poly-adenine (poly-A) tail added to the 3’ end, 100 or more adenine nucleotides

ijvtpr.com/index.php/IJVT…

This modification also increases the translation of the mRNA, resulting in the protein product being synthesized, i.e., the spike-protein actually being manufactured.

ncbi.nlm.nih.gov/pmc/articles/P…

sciencedirect.com/science/articl…

This is very concerning with regards to infant nursing exposure, given the evidence that breastmilk EVs and their cargo (containing RNAs) not only survive the stomach...

sciencedirect.com/science/articl…

...but also make it to the brain via the vagus nerve and those not absorbed in the intestines remain there, biologically active.

nature.com/articles/s4159…

The vaccine-mRNA has also been enriched with two of the main DNA base building blocks guanines (G) and cytosines (C), this modification further increases transcription production of the biologically active spike-protein.

ncbi.nlm.nih.gov/pmc/articles/P…

ncbi.nlm.nih.gov/pmc/articles/P…

Viruses tend to have lower GC content than the host genome, making them sensitive to an increase in body temperature, which we get and aids in fighting an infection….

link.springer.com/article/10.100…

The modification of increasing the GC content in the vaccine-mRNA increases its heat stability, rendering our natural infection fighting process, such as running a fever to facilitate breaking down of viral mRNA, ineffective...

If there is presence of commercially produced SARS-CoV-2 S-protein-coding vaccine modified-mRNA in human breast milk, it is highly likely to survive the infant’s digestive tract, as human breast milk is enriched with EVs, and – as discussed above –

…survive the digestive tract and bioaccumulate in the brain. Milk EVs have been measured/detected in the blood plasma post prandial (after feeding), and those that do not get absorbed have been shown to affect gene transcription in the intestinal tract.

....Conclusions
We are supposed to always err on the side of caution. This means implementing the principle of “First, Do No Harm”. Experimental interventions should not be made that do not have robust evidence of safety as well as efficacy.

This is exactly why “whole of life” animal studies, including pregnancy and lactation animal studies must be completed BEFORE implementing human trials of any sorts, as per according to point 3 of the Nuremberg code. This was not done.

ncbi.nlm.nih.gov/pmc/articles/P…

Finally, the human trials have not even reached a full year yet, did not include pregnant or lactating women, nor nursing infants.

As a result, no safety can be ascertained, and therefore should not be claimed.