I am shocked at how expensive these C19 tests are ($100-$300).
And no...The free ones are not free!
They are printing money, diluting yours and paying a heavy price you just can't see.
Diagnostic PCR is usually $30.
Even with 10X higher margins on C19 PCR, there are still droughts after 2 years?
Why?
This is the story of central planning where the market gets run by whimsical gov diktat and pricing signals are destroyed.
When pricing signals are destroyed companies dont know how to plan
Will Biden have an aneurism or an epiphany today and order 500M tests they fumble?
Will schools panic on Omicron and order more school testing of asympto kids who don't get sick?
When you run the market by Fiat, you create artificial demand but you don't create supply.
Supply is forecasted with pricing signals.
If you can't get a C19 test that is marked up 10X from traditional PCR costs.... good luck getting a cancer PCR test that is competing for the same nucleotides and polymerases.
These deaths wont be televised
Central planning always leads to starvation.
Today, we get to see how the central planners starve society of polymerases and nucleotides by destroying price signals.
But this topic is a century old and power hungry people always try to bury it mises.org/library/econom…
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2)pseudouridine and N1-methyl should be better spelled out. Xia et al conflates the two and we will spell this out more.
PseudoU wobbles more the methylpseudoU but both significantly alter Tm and thus are ‘stickier’ bases than disrupt translation.
Very thorough.
Not only applied a live-dead like PCR looking at sgRNA, they also cultured the virus and looked for immune histochemistry confirmation of proteins.
Great chat with @jjcouey
I have some errors to confess to as I spoke to fast.
1)I was enrolled in a PhD program at UW but dropped out to focus on my job when the HGP starting racing with Craig Venter. So No PhD.
3)The vaccines have N1-Methyl Pseudouridine and I shouldn't shorthand this to Pseudouridine as the former has less wobble than the latter. N1-Methyl does alter the Tm and increase base stacking in RNA but its methyl group steals one potential H-bond.
Let’s have a look at the methods section of the paper.
2 rounds of 45 cycles of PCR?
This is what you do if you want to make polymerase slippage errors near GC rich triplet repeats.
People this library illiterate shouldn’t have the keys to $20K NovaSeq runs.
Having engineered very accurate sequencers, to address PCR polymerase error, you must utilize DNA barcodes that get attached to your PCR products in the first cycle of PCR. Any barcode that over amplifies through the PCR process is discounted.