I like mice…
So does some lab that derived Omicron.
Very good read.
You’ll see how Molnupiravir could not have done this alone.
Random C->U mutations are not elevated in omicron but GC-AT are different in early omicron Vs post outbreak.
Mice host!
GC-AT mutations are a signature of ROS damage that makes 8-OxoG.
ROS is elevated in febrile viral infections.
These authors looked at early omicron Vs post outbreak omicron and see a difference here suggesting omicron had a different host at some point.
Mice fit the bill.
Another kind cat sent me this paper.
Another important point is the Ka/Ks analysis they do (dN/dS).
This shows 6.6X higher selective pressure on spike but not on the rest of the SARs genome.
Mice + selection against spike (vax of MAbs) could do this.
2)pseudouridine and N1-methyl should be better spelled out. Xia et al conflates the two and we will spell this out more.
PseudoU wobbles more the methylpseudoU but both significantly alter Tm and thus are ‘stickier’ bases than disrupt translation.
Very thorough.
Not only applied a live-dead like PCR looking at sgRNA, they also cultured the virus and looked for immune histochemistry confirmation of proteins.
Great chat with @jjcouey
I have some errors to confess to as I spoke to fast.
1)I was enrolled in a PhD program at UW but dropped out to focus on my job when the HGP starting racing with Craig Venter. So No PhD.
3)The vaccines have N1-Methyl Pseudouridine and I shouldn't shorthand this to Pseudouridine as the former has less wobble than the latter. N1-Methyl does alter the Tm and increase base stacking in RNA but its methyl group steals one potential H-bond.